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Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells

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Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells. / Fijak, Monika; Damm, Lara-Jil; Wenzel, Jan-Per; Aslani, Ferial; Walecki, Magdalena; Wahle, Eva; Eisel, Florian; Bhushan, Sudhanshu; Hackstein, Holger; Baal, Nelli; Schuler, Gerhard; Konrad, Lutz; Rafiq, Amir; O'Hara, Laura; Smith, Lee B; Meinhardt, Andreas.

in: AM J REPROD IMMUNOL, Jahrgang 74, Nr. 1, 07.2015, S. 12-25.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Fijak, M, Damm, L-J, Wenzel, J-P, Aslani, F, Walecki, M, Wahle, E, Eisel, F, Bhushan, S, Hackstein, H, Baal, N, Schuler, G, Konrad, L, Rafiq, A, O'Hara, L, Smith, LB & Meinhardt, A 2015, 'Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells', AM J REPROD IMMUNOL, Jg. 74, Nr. 1, S. 12-25. https://doi.org/10.1111/aji.12363

APA

Fijak, M., Damm, L-J., Wenzel, J-P., Aslani, F., Walecki, M., Wahle, E., Eisel, F., Bhushan, S., Hackstein, H., Baal, N., Schuler, G., Konrad, L., Rafiq, A., O'Hara, L., Smith, L. B., & Meinhardt, A. (2015). Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells. AM J REPROD IMMUNOL, 74(1), 12-25. https://doi.org/10.1111/aji.12363

Vancouver

Fijak M, Damm L-J, Wenzel J-P, Aslani F, Walecki M, Wahle E et al. Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells. AM J REPROD IMMUNOL. 2015 Jul;74(1):12-25. https://doi.org/10.1111/aji.12363

Bibtex

@article{abdee5441e0d4bd9a1d953a1c3097a82,
title = "Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells",
abstract = "PROBLEM: Previous studies demonstrated a strong association between low androgen levels and reduced capacity to mount an inflammatory response. However, the mechanisms underlying these observations are largely not understood.METHODS OF STUDY: Generation of CD4+CD25+Foxp3+ regulatory T cells in Leydig cell-conditioned media was determined by flow cytometry and ELISA. Influence of testosterone on cytokine response was measured in LPS-stimulated testicular macrophages, Sertoli and peritubular cells.RESULTS: Leydig cell-conditioned media dose-dependently stimulated expression of transcription factor Foxp3 and secretion of IL-10 in splenic CD4+ T cells, an effect abolished by addition of the anti-androgen flutamide. In isolated Sertoli and peritubular cells, testosterone pre-treatment suppressed the LPS-induced inflammatory response on TNF-α mRNA expression, while no effect was evident in testicular macrophages (TM).CONCLUSIONS: Androgens can influence the immune system under normal conditions by the generation and functional differentiation of regulatory T cells and in testicular inflammation by direct effect on Sertoli and peritubular cells.",
keywords = "Androgen Antagonists/pharmacology, Animals, Cell Differentiation/drug effects, Cells, Cultured, Chemokine CCL2/biosynthesis, Culture Media, Conditioned/pharmacology, Flutamide/pharmacology, Forkhead Transcription Factors/biosynthesis, Inflammation/immunology, Interleukin-10/biosynthesis, Leydig Cells/immunology, Macrophages/immunology, Male, RNA, Messenger/biosynthesis, Rats, Rats, Wistar, Sertoli Cells/immunology, T-Lymphocytes, Regulatory/cytology, Testosterone/antagonists & inhibitors, Transforming Growth Factor beta/biosynthesis, Tumor Necrosis Factor-alpha/genetics",
author = "Monika Fijak and Lara-Jil Damm and Jan-Per Wenzel and Ferial Aslani and Magdalena Walecki and Eva Wahle and Florian Eisel and Sudhanshu Bhushan and Holger Hackstein and Nelli Baal and Gerhard Schuler and Lutz Konrad and Amir Rafiq and Laura O'Hara and Smith, {Lee B} and Andreas Meinhardt",
note = "{\textcopyright} 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.",
year = "2015",
month = jul,
doi = "10.1111/aji.12363",
language = "English",
volume = "74",
pages = "12--25",
journal = "AM J REPROD IMMUNOL",
issn = "1046-7408",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells

AU - Fijak, Monika

AU - Damm, Lara-Jil

AU - Wenzel, Jan-Per

AU - Aslani, Ferial

AU - Walecki, Magdalena

AU - Wahle, Eva

AU - Eisel, Florian

AU - Bhushan, Sudhanshu

AU - Hackstein, Holger

AU - Baal, Nelli

AU - Schuler, Gerhard

AU - Konrad, Lutz

AU - Rafiq, Amir

AU - O'Hara, Laura

AU - Smith, Lee B

AU - Meinhardt, Andreas

N1 - © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

PY - 2015/7

Y1 - 2015/7

N2 - PROBLEM: Previous studies demonstrated a strong association between low androgen levels and reduced capacity to mount an inflammatory response. However, the mechanisms underlying these observations are largely not understood.METHODS OF STUDY: Generation of CD4+CD25+Foxp3+ regulatory T cells in Leydig cell-conditioned media was determined by flow cytometry and ELISA. Influence of testosterone on cytokine response was measured in LPS-stimulated testicular macrophages, Sertoli and peritubular cells.RESULTS: Leydig cell-conditioned media dose-dependently stimulated expression of transcription factor Foxp3 and secretion of IL-10 in splenic CD4+ T cells, an effect abolished by addition of the anti-androgen flutamide. In isolated Sertoli and peritubular cells, testosterone pre-treatment suppressed the LPS-induced inflammatory response on TNF-α mRNA expression, while no effect was evident in testicular macrophages (TM).CONCLUSIONS: Androgens can influence the immune system under normal conditions by the generation and functional differentiation of regulatory T cells and in testicular inflammation by direct effect on Sertoli and peritubular cells.

AB - PROBLEM: Previous studies demonstrated a strong association between low androgen levels and reduced capacity to mount an inflammatory response. However, the mechanisms underlying these observations are largely not understood.METHODS OF STUDY: Generation of CD4+CD25+Foxp3+ regulatory T cells in Leydig cell-conditioned media was determined by flow cytometry and ELISA. Influence of testosterone on cytokine response was measured in LPS-stimulated testicular macrophages, Sertoli and peritubular cells.RESULTS: Leydig cell-conditioned media dose-dependently stimulated expression of transcription factor Foxp3 and secretion of IL-10 in splenic CD4+ T cells, an effect abolished by addition of the anti-androgen flutamide. In isolated Sertoli and peritubular cells, testosterone pre-treatment suppressed the LPS-induced inflammatory response on TNF-α mRNA expression, while no effect was evident in testicular macrophages (TM).CONCLUSIONS: Androgens can influence the immune system under normal conditions by the generation and functional differentiation of regulatory T cells and in testicular inflammation by direct effect on Sertoli and peritubular cells.

KW - Androgen Antagonists/pharmacology

KW - Animals

KW - Cell Differentiation/drug effects

KW - Cells, Cultured

KW - Chemokine CCL2/biosynthesis

KW - Culture Media, Conditioned/pharmacology

KW - Flutamide/pharmacology

KW - Forkhead Transcription Factors/biosynthesis

KW - Inflammation/immunology

KW - Interleukin-10/biosynthesis

KW - Leydig Cells/immunology

KW - Macrophages/immunology

KW - Male

KW - RNA, Messenger/biosynthesis

KW - Rats

KW - Rats, Wistar

KW - Sertoli Cells/immunology

KW - T-Lymphocytes, Regulatory/cytology

KW - Testosterone/antagonists & inhibitors

KW - Transforming Growth Factor beta/biosynthesis

KW - Tumor Necrosis Factor-alpha/genetics

U2 - 10.1111/aji.12363

DO - 10.1111/aji.12363

M3 - SCORING: Journal article

C2 - 25598450

VL - 74

SP - 12

EP - 25

JO - AM J REPROD IMMUNOL

JF - AM J REPROD IMMUNOL

SN - 1046-7408

IS - 1

ER -