Influence of slide aging on results of translational research studies using immunohistochemistry.
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Influence of slide aging on results of translational research studies using immunohistochemistry. / Mirlacher, Martina; Kasper, Marlis; Storz, Martina; Knecht, Yvonne; Dürmüller, Ursula; Simon, Ronald; Mihatsch, Michael J; Sauter, Guido.
in: MODERN PATHOL, Jahrgang 17, Nr. 11, 11, 2004, S. 1414-1420.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Influence of slide aging on results of translational research studies using immunohistochemistry.
AU - Mirlacher, Martina
AU - Kasper, Marlis
AU - Storz, Martina
AU - Knecht, Yvonne
AU - Dürmüller, Ursula
AU - Simon, Ronald
AU - Mihatsch, Michael J
AU - Sauter, Guido
PY - 2004
Y1 - 2004
N2 - Several reports have shown that a long delay between cutting sections and immunohistochemical (IHC) staining can decrease the IHC reaction intensity. However, systematic large-scale studies to investigate to what extent this problem may influence the outcome of translational research studies are lacking. In this study, we used a tissue microarray (TMA) approach to investigate the influence of slide age on comparisons between the results of IHC analyses for estrogen receptor (ER), progesterone receptor (PR), cyclin D1, HER2 (HercepTest), and E-cadherin and clinical outcome in a series of 522 breast cancer patients. Old TMA sections stored for 6 months at 4 degrees C and freshly cut sections were analyzed under exactly identical experimental conditions. As compared to results obtained on freshly cut sections, the frequency of positivity on old sections decreased from 65 to 46% for ER (P
AB - Several reports have shown that a long delay between cutting sections and immunohistochemical (IHC) staining can decrease the IHC reaction intensity. However, systematic large-scale studies to investigate to what extent this problem may influence the outcome of translational research studies are lacking. In this study, we used a tissue microarray (TMA) approach to investigate the influence of slide age on comparisons between the results of IHC analyses for estrogen receptor (ER), progesterone receptor (PR), cyclin D1, HER2 (HercepTest), and E-cadherin and clinical outcome in a series of 522 breast cancer patients. Old TMA sections stored for 6 months at 4 degrees C and freshly cut sections were analyzed under exactly identical experimental conditions. As compared to results obtained on freshly cut sections, the frequency of positivity on old sections decreased from 65 to 46% for ER (P
M3 - SCORING: Zeitschriftenaufsatz
VL - 17
SP - 1414
EP - 1420
JO - MODERN PATHOL
JF - MODERN PATHOL
SN - 0893-3952
IS - 11
M1 - 11
ER -
