Influence of Birch Bark Triterpenes on Keratinocytes and Fibroblasts from Diabetic and Nondiabetic Donors
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Influence of Birch Bark Triterpenes on Keratinocytes and Fibroblasts from Diabetic and Nondiabetic Donors. / Wardecki, Tina; Werner, Philipp; Thomas, Maria; Templin, Markus F; Schmidt, Gudula; Brandner, Johanna M; Merfort, Irmgard.
in: J NAT PROD, Jahrgang 79, Nr. 4, 22.04.2016, S. 1112-23.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Influence of Birch Bark Triterpenes on Keratinocytes and Fibroblasts from Diabetic and Nondiabetic Donors
AU - Wardecki, Tina
AU - Werner, Philipp
AU - Thomas, Maria
AU - Templin, Markus F
AU - Schmidt, Gudula
AU - Brandner, Johanna M
AU - Merfort, Irmgard
PY - 2016/4/22
Y1 - 2016/4/22
N2 - Impaired wound healing is one of the main risk factors associated with diabetes mellitus. Few options are available to treat diabetic wounds, and therefore efficient remedies are urgently needed. An interesting option might be an extract of birch bark (TE) that has been clinically proven to accelerate acute wound healing. We investigated the effects of TE and its main components betulin and lupeol in cultured normal keratinocytes and dermal fibroblasts from diabetic and nondiabetic donors. These in vitro models can provide insights into possible beneficial effects in wound healing. TE and betulin treatment led to increased mRNA levels of chemokines, pro-inflammatory cytokines, and mediators important in wound healing, e.g., IL-6, TNFα, IL-8, and RANTES. We observed a pronounced upregulation of MIF, IL-8, and RANTES on the protein level. Furthermore, a shape change of the actin cytoskeleton was seen in keratinocytes and fibroblasts, and the Rho-GTPases and p38-MAPK were found to be activated in keratinocytes. On the basis of our results, TE is worthy of further study as a potential option to influence wound-healing processes under diabetic conditions. These first insights need to be confirmed by clinical studies with diabetic patients.
AB - Impaired wound healing is one of the main risk factors associated with diabetes mellitus. Few options are available to treat diabetic wounds, and therefore efficient remedies are urgently needed. An interesting option might be an extract of birch bark (TE) that has been clinically proven to accelerate acute wound healing. We investigated the effects of TE and its main components betulin and lupeol in cultured normal keratinocytes and dermal fibroblasts from diabetic and nondiabetic donors. These in vitro models can provide insights into possible beneficial effects in wound healing. TE and betulin treatment led to increased mRNA levels of chemokines, pro-inflammatory cytokines, and mediators important in wound healing, e.g., IL-6, TNFα, IL-8, and RANTES. We observed a pronounced upregulation of MIF, IL-8, and RANTES on the protein level. Furthermore, a shape change of the actin cytoskeleton was seen in keratinocytes and fibroblasts, and the Rho-GTPases and p38-MAPK were found to be activated in keratinocytes. On the basis of our results, TE is worthy of further study as a potential option to influence wound-healing processes under diabetic conditions. These first insights need to be confirmed by clinical studies with diabetic patients.
KW - Betula
KW - Cytokines
KW - Diabetes Mellitus
KW - Female
KW - Fibroblasts
KW - Humans
KW - Interleukin-6
KW - Interleukin-8
KW - Keratinocytes
KW - Pentacyclic Triterpenes
KW - Plant Bark
KW - Triterpenes
KW - Wound Healing
KW - p38 Mitogen-Activated Protein Kinases
KW - rho GTP-Binding Proteins
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1021/acs.jnatprod.6b00027
DO - 10.1021/acs.jnatprod.6b00027
M3 - SCORING: Journal article
C2 - 27002382
VL - 79
SP - 1112
EP - 1123
JO - J NAT PROD
JF - J NAT PROD
SN - 0163-3864
IS - 4
ER -
