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Infection and transmission dynamics of rKSHV.219 in primary endothelial cells

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Infection and transmission dynamics of rKSHV.219 in primary endothelial cells. / Jeffery, Hannah C; Wheat, Rachel L; Blackbourn, David J; Nash, Gerard B; Butler, Lynn M.

in: J VIROL METHODS, Jahrgang 193, Nr. 1, 10.2013, S. 251-9.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Jeffery, HC, Wheat, RL, Blackbourn, DJ, Nash, GB & Butler, LM 2013, 'Infection and transmission dynamics of rKSHV.219 in primary endothelial cells', J VIROL METHODS, Jg. 193, Nr. 1, S. 251-9. https://doi.org/10.1016/j.jviromet.2013.06.001

APA

Jeffery, H. C., Wheat, R. L., Blackbourn, D. J., Nash, G. B., & Butler, L. M. (2013). Infection and transmission dynamics of rKSHV.219 in primary endothelial cells. J VIROL METHODS, 193(1), 251-9. https://doi.org/10.1016/j.jviromet.2013.06.001

Vancouver

Jeffery HC, Wheat RL, Blackbourn DJ, Nash GB, Butler LM. Infection and transmission dynamics of rKSHV.219 in primary endothelial cells. J VIROL METHODS. 2013 Okt;193(1):251-9. https://doi.org/10.1016/j.jviromet.2013.06.001

Bibtex

@article{2ebf730a191e4604b5be1dbf75fe4c55,
title = "Infection and transmission dynamics of rKSHV.219 in primary endothelial cells",
abstract = "Kaposi's sarcoma-associated herpesvirus (KSHV) is the aetiologic agent of Kaposi's sarcoma (KS), a tumour of endothelial cell origin. The study of KS development was aided by the generation of a recombinant GFP (latent)/RFP (lytic)-expressing KSHV (rKSHV.219) by Vieira and O'Hearn (2004). In this study the first data characterising primary endothelial cell infection and transmission with this virus is presented. Infection was predominantly latent and the percentage of GFP-positive cells increased over time. Neither horizontal transmission of infection, nor cellular proliferation, explained this increase. Analysis of latency-associated nuclear antigen (LANA-1) expression revealed that a threshold level of infection was required for GFP expression early post infection. At later time points GFP correlated more closely with LANA-1 expression, likely due to the accumulation of GFP over time. This study provides methodological guidance for the use of rKSHV.21. In addition, it highlights potential problems associated with the use of fluorescent proteins as markers of viral infection.",
keywords = "Cells, Cultured, Endothelial Cells, Fluorescence, Green Fluorescent Proteins, Herpesvirus 8, Human, Humans, Staining and Labeling, Time Factors, Virology, Virus Latency, Virus Replication",
author = "Jeffery, {Hannah C} and Wheat, {Rachel L} and Blackbourn, {David J} and Nash, {Gerard B} and Butler, {Lynn M}",
note = "Copyright {\textcopyright} 2013 Elsevier B.V. All rights reserved.",
year = "2013",
month = oct,
doi = "10.1016/j.jviromet.2013.06.001",
language = "English",
volume = "193",
pages = "251--9",
journal = "J VIROL METHODS",
issn = "0166-0934",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Infection and transmission dynamics of rKSHV.219 in primary endothelial cells

AU - Jeffery, Hannah C

AU - Wheat, Rachel L

AU - Blackbourn, David J

AU - Nash, Gerard B

AU - Butler, Lynn M

N1 - Copyright © 2013 Elsevier B.V. All rights reserved.

PY - 2013/10

Y1 - 2013/10

N2 - Kaposi's sarcoma-associated herpesvirus (KSHV) is the aetiologic agent of Kaposi's sarcoma (KS), a tumour of endothelial cell origin. The study of KS development was aided by the generation of a recombinant GFP (latent)/RFP (lytic)-expressing KSHV (rKSHV.219) by Vieira and O'Hearn (2004). In this study the first data characterising primary endothelial cell infection and transmission with this virus is presented. Infection was predominantly latent and the percentage of GFP-positive cells increased over time. Neither horizontal transmission of infection, nor cellular proliferation, explained this increase. Analysis of latency-associated nuclear antigen (LANA-1) expression revealed that a threshold level of infection was required for GFP expression early post infection. At later time points GFP correlated more closely with LANA-1 expression, likely due to the accumulation of GFP over time. This study provides methodological guidance for the use of rKSHV.21. In addition, it highlights potential problems associated with the use of fluorescent proteins as markers of viral infection.

AB - Kaposi's sarcoma-associated herpesvirus (KSHV) is the aetiologic agent of Kaposi's sarcoma (KS), a tumour of endothelial cell origin. The study of KS development was aided by the generation of a recombinant GFP (latent)/RFP (lytic)-expressing KSHV (rKSHV.219) by Vieira and O'Hearn (2004). In this study the first data characterising primary endothelial cell infection and transmission with this virus is presented. Infection was predominantly latent and the percentage of GFP-positive cells increased over time. Neither horizontal transmission of infection, nor cellular proliferation, explained this increase. Analysis of latency-associated nuclear antigen (LANA-1) expression revealed that a threshold level of infection was required for GFP expression early post infection. At later time points GFP correlated more closely with LANA-1 expression, likely due to the accumulation of GFP over time. This study provides methodological guidance for the use of rKSHV.21. In addition, it highlights potential problems associated with the use of fluorescent proteins as markers of viral infection.

KW - Cells, Cultured

KW - Endothelial Cells

KW - Fluorescence

KW - Green Fluorescent Proteins

KW - Herpesvirus 8, Human

KW - Humans

KW - Staining and Labeling

KW - Time Factors

KW - Virology

KW - Virus Latency

KW - Virus Replication

U2 - 10.1016/j.jviromet.2013.06.001

DO - 10.1016/j.jviromet.2013.06.001

M3 - SCORING: Journal article

C2 - 23764419

VL - 193

SP - 251

EP - 259

JO - J VIROL METHODS

JF - J VIROL METHODS

SN - 0166-0934

IS - 1

ER -