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Induction of intercellular adhesion molecule-1 but not of lymphocyte function-associated antigen-3 in thyroid follicular cells

Standard

Induction of intercellular adhesion molecule-1 but not of lymphocyte function-associated antigen-3 in thyroid follicular cells. / Tolosa, E; Roura, C; Martí, M; Belfiore, A; Pujol-Borrell, R.

in: J AUTOIMMUN, Jahrgang 5, Nr. 1, 01.02.1992, S. 119-35.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Tolosa, E, Roura, C, Martí, M, Belfiore, A & Pujol-Borrell, R 1992, 'Induction of intercellular adhesion molecule-1 but not of lymphocyte function-associated antigen-3 in thyroid follicular cells', J AUTOIMMUN, Jg. 5, Nr. 1, S. 119-35.

APA

Tolosa, E., Roura, C., Martí, M., Belfiore, A., & Pujol-Borrell, R. (1992). Induction of intercellular adhesion molecule-1 but not of lymphocyte function-associated antigen-3 in thyroid follicular cells. J AUTOIMMUN, 5(1), 119-35.

Vancouver

Tolosa E, Roura C, Martí M, Belfiore A, Pujol-Borrell R. Induction of intercellular adhesion molecule-1 but not of lymphocyte function-associated antigen-3 in thyroid follicular cells. J AUTOIMMUN. 1992 Feb 1;5(1):119-35.

Bibtex

@article{d679f2b7e59a4da79c50c8f6d685319d,
title = "Induction of intercellular adhesion molecule-1 but not of lymphocyte function-associated antigen-3 in thyroid follicular cells",
abstract = "We investigated the expression of intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-3 (LFA-3) by thyrocytes and their regulation by cytokines. Immunofluorescence studies on cryostat sections and on freshly dispersed cell preparations showed that ICAM-1 and LFA-3 are barely detectable in non-autoimmune thyrocytes. However, thyrocytes acquired ICAM-1 expression in culture. IFN-gamma, IL-1 beta and TNF-alpha produced a clear enhancement of ICAM-1 expression. When tested in combination, IL-1 beta and TNF-alpha were additive to the IFN-gamma effect. LFA-3 expression was not modulated by these cytokines. In the HT93 thyroid cell line generated by transfection with SV40, ICAM-1 and LFA-3 were both constitutively expressed at high levels. Cytokines modulated ICAM-1 expression similarly, but to a greater extent than in normal thyrocytes. LFA-3 remained unmodified. These results support the notion that normal thyrocytes are immunologically silent cells. The capability of cytokines to induce ICAM-1 together with HLA class I and class II-expression on thyrocytes suggests that under their influence, these cells may express all the surface molecules required for antigen presentation and/or for being recognized as target cells in the context of thyroid autoimmune disease.",
keywords = "Antibodies, Monoclonal, Antigens, CD58, Antigens, Surface, Blotting, Northern, Cell Adhesion Molecules, Cell Line, Cytokines, Female, Flow Cytometry, Fluorescent Antibody Technique, Goiter, Nodular, HLA-DR Antigens, Histocompatibility Antigens Class I, Humans, Intercellular Adhesion Molecule-1, Membrane Glycoproteins, Thyroid Gland",
author = "E Tolosa and C Roura and M Mart{\'i} and A Belfiore and R Pujol-Borrell",
year = "1992",
month = feb,
day = "1",
language = "English",
volume = "5",
pages = "119--35",
journal = "J AUTOIMMUN",
issn = "0896-8411",
publisher = "Academic Press Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Induction of intercellular adhesion molecule-1 but not of lymphocyte function-associated antigen-3 in thyroid follicular cells

AU - Tolosa, E

AU - Roura, C

AU - Martí, M

AU - Belfiore, A

AU - Pujol-Borrell, R

PY - 1992/2/1

Y1 - 1992/2/1

N2 - We investigated the expression of intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-3 (LFA-3) by thyrocytes and their regulation by cytokines. Immunofluorescence studies on cryostat sections and on freshly dispersed cell preparations showed that ICAM-1 and LFA-3 are barely detectable in non-autoimmune thyrocytes. However, thyrocytes acquired ICAM-1 expression in culture. IFN-gamma, IL-1 beta and TNF-alpha produced a clear enhancement of ICAM-1 expression. When tested in combination, IL-1 beta and TNF-alpha were additive to the IFN-gamma effect. LFA-3 expression was not modulated by these cytokines. In the HT93 thyroid cell line generated by transfection with SV40, ICAM-1 and LFA-3 were both constitutively expressed at high levels. Cytokines modulated ICAM-1 expression similarly, but to a greater extent than in normal thyrocytes. LFA-3 remained unmodified. These results support the notion that normal thyrocytes are immunologically silent cells. The capability of cytokines to induce ICAM-1 together with HLA class I and class II-expression on thyrocytes suggests that under their influence, these cells may express all the surface molecules required for antigen presentation and/or for being recognized as target cells in the context of thyroid autoimmune disease.

AB - We investigated the expression of intercellular adhesion molecule-1 (ICAM-1) and lymphocyte function-associated antigen-3 (LFA-3) by thyrocytes and their regulation by cytokines. Immunofluorescence studies on cryostat sections and on freshly dispersed cell preparations showed that ICAM-1 and LFA-3 are barely detectable in non-autoimmune thyrocytes. However, thyrocytes acquired ICAM-1 expression in culture. IFN-gamma, IL-1 beta and TNF-alpha produced a clear enhancement of ICAM-1 expression. When tested in combination, IL-1 beta and TNF-alpha were additive to the IFN-gamma effect. LFA-3 expression was not modulated by these cytokines. In the HT93 thyroid cell line generated by transfection with SV40, ICAM-1 and LFA-3 were both constitutively expressed at high levels. Cytokines modulated ICAM-1 expression similarly, but to a greater extent than in normal thyrocytes. LFA-3 remained unmodified. These results support the notion that normal thyrocytes are immunologically silent cells. The capability of cytokines to induce ICAM-1 together with HLA class I and class II-expression on thyrocytes suggests that under their influence, these cells may express all the surface molecules required for antigen presentation and/or for being recognized as target cells in the context of thyroid autoimmune disease.

KW - Antibodies, Monoclonal

KW - Antigens, CD58

KW - Antigens, Surface

KW - Blotting, Northern

KW - Cell Adhesion Molecules

KW - Cell Line

KW - Cytokines

KW - Female

KW - Flow Cytometry

KW - Fluorescent Antibody Technique

KW - Goiter, Nodular

KW - HLA-DR Antigens

KW - Histocompatibility Antigens Class I

KW - Humans

KW - Intercellular Adhesion Molecule-1

KW - Membrane Glycoproteins

KW - Thyroid Gland

M3 - SCORING: Journal article

C2 - 1373059

VL - 5

SP - 119

EP - 135

JO - J AUTOIMMUN

JF - J AUTOIMMUN

SN - 0896-8411

IS - 1

ER -