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Inducible costimulator protein controls the protective T cell response against Listeria monocytogenes

Standard

Inducible costimulator protein controls the protective T cell response against Listeria monocytogenes. / Mittrücker, Hans-Willi; Kursar, Mischo; Köhler, Anne; Yanagihara, Donna; Yoshinaga, Steven K; Kaufmann, Stefan H E.

in: J IMMUNOL, Jahrgang 169, Nr. 10, 15.11.2002, S. 5813-7.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Mittrücker, H-W, Kursar, M, Köhler, A, Yanagihara, D, Yoshinaga, SK & Kaufmann, SHE 2002, 'Inducible costimulator protein controls the protective T cell response against Listeria monocytogenes', J IMMUNOL, Jg. 169, Nr. 10, S. 5813-7.

APA

Mittrücker, H-W., Kursar, M., Köhler, A., Yanagihara, D., Yoshinaga, S. K., & Kaufmann, S. H. E. (2002). Inducible costimulator protein controls the protective T cell response against Listeria monocytogenes. J IMMUNOL, 169(10), 5813-7.

Vancouver

Mittrücker H-W, Kursar M, Köhler A, Yanagihara D, Yoshinaga SK, Kaufmann SHE. Inducible costimulator protein controls the protective T cell response against Listeria monocytogenes. J IMMUNOL. 2002 Nov 15;169(10):5813-7.

Bibtex

@article{39ead1e100e74567810f51b944eef1fb,
title = "Inducible costimulator protein controls the protective T cell response against Listeria monocytogenes",
abstract = "The inducible costimulator protein (ICOS) was recently identified as a costimulatory molecule for T cells. Here we analyze the role of ICOS for the acquired immune response of mice against the intracellular bacterium Listeria monocytogenes. During oral L. monocytogenes infection, low levels of ICOS expression were detected by extracellular and intracellular Ab staining of Listeria-specific CD4(+) and CD8(+) T cells. Blocking of ICOS signaling with a soluble ICOS-Ig fusion protein markedly impaired the Listeria-specific T cell responses. Compared with control mice, the ICOS-Ig treated mice generated significantly reduced numbers of Listeria-specific CD8(+) T cells in spleen and liver, as determined by tetramer and intracellular cytokine staining. In contrast, the specific CD8(+) T cell response in the intestinal mucosa did not appear to be impaired by the ICOS-Ig treatment. Analysis of the CD4(+) T cell response revealed that ICOS-Ig treatment also affected the specific CD4(+) T cell response. When restimulated with listerial Ag in vitro, reduced numbers of CD4(+) T cells from infected and ICOS-Ig-treated mice responded with IFN-gamma production. The impaired acquired immune response in ICOS-Ig treated mice was accompanied by their increased susceptibility to L. monocytogenes infection. ICOS-Ig treatment drastically enhanced bacterial titers, and a large fraction of mice succumbed to the otherwise sublethal dose of infection. Thus, ICOS costimulation is crucial for protective immunity against the intracellular bacterium L. monocytogenes.",
keywords = "Administration, Oral, Animals, Antibodies, Blocking, Antibodies, Monoclonal, Antigens, Differentiation, T-Lymphocyte, CD4-CD8 Ratio, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Disease Susceptibility, Epitopes, T-Lymphocyte, Humans, Inducible T-Cell Co-Stimulator Protein, Injections, Intravenous, Injections, Subcutaneous, Interferon-gamma, Listeria monocytogenes, Listeriosis, Lymphocyte Activation, Lymphocyte Depletion, Mice, Mice, Inbred BALB C, Rats, Rats, Inbred Strains, Recombinant Fusion Proteins, Signal Transduction, T-Lymphocyte Subsets",
author = "Hans-Willi Mittr{\"u}cker and Mischo Kursar and Anne K{\"o}hler and Donna Yanagihara and Yoshinaga, {Steven K} and Kaufmann, {Stefan H E}",
year = "2002",
month = nov,
day = "15",
language = "English",
volume = "169",
pages = "5813--7",
journal = "J IMMUNOL",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "10",

}

RIS

TY - JOUR

T1 - Inducible costimulator protein controls the protective T cell response against Listeria monocytogenes

AU - Mittrücker, Hans-Willi

AU - Kursar, Mischo

AU - Köhler, Anne

AU - Yanagihara, Donna

AU - Yoshinaga, Steven K

AU - Kaufmann, Stefan H E

PY - 2002/11/15

Y1 - 2002/11/15

N2 - The inducible costimulator protein (ICOS) was recently identified as a costimulatory molecule for T cells. Here we analyze the role of ICOS for the acquired immune response of mice against the intracellular bacterium Listeria monocytogenes. During oral L. monocytogenes infection, low levels of ICOS expression were detected by extracellular and intracellular Ab staining of Listeria-specific CD4(+) and CD8(+) T cells. Blocking of ICOS signaling with a soluble ICOS-Ig fusion protein markedly impaired the Listeria-specific T cell responses. Compared with control mice, the ICOS-Ig treated mice generated significantly reduced numbers of Listeria-specific CD8(+) T cells in spleen and liver, as determined by tetramer and intracellular cytokine staining. In contrast, the specific CD8(+) T cell response in the intestinal mucosa did not appear to be impaired by the ICOS-Ig treatment. Analysis of the CD4(+) T cell response revealed that ICOS-Ig treatment also affected the specific CD4(+) T cell response. When restimulated with listerial Ag in vitro, reduced numbers of CD4(+) T cells from infected and ICOS-Ig-treated mice responded with IFN-gamma production. The impaired acquired immune response in ICOS-Ig treated mice was accompanied by their increased susceptibility to L. monocytogenes infection. ICOS-Ig treatment drastically enhanced bacterial titers, and a large fraction of mice succumbed to the otherwise sublethal dose of infection. Thus, ICOS costimulation is crucial for protective immunity against the intracellular bacterium L. monocytogenes.

AB - The inducible costimulator protein (ICOS) was recently identified as a costimulatory molecule for T cells. Here we analyze the role of ICOS for the acquired immune response of mice against the intracellular bacterium Listeria monocytogenes. During oral L. monocytogenes infection, low levels of ICOS expression were detected by extracellular and intracellular Ab staining of Listeria-specific CD4(+) and CD8(+) T cells. Blocking of ICOS signaling with a soluble ICOS-Ig fusion protein markedly impaired the Listeria-specific T cell responses. Compared with control mice, the ICOS-Ig treated mice generated significantly reduced numbers of Listeria-specific CD8(+) T cells in spleen and liver, as determined by tetramer and intracellular cytokine staining. In contrast, the specific CD8(+) T cell response in the intestinal mucosa did not appear to be impaired by the ICOS-Ig treatment. Analysis of the CD4(+) T cell response revealed that ICOS-Ig treatment also affected the specific CD4(+) T cell response. When restimulated with listerial Ag in vitro, reduced numbers of CD4(+) T cells from infected and ICOS-Ig-treated mice responded with IFN-gamma production. The impaired acquired immune response in ICOS-Ig treated mice was accompanied by their increased susceptibility to L. monocytogenes infection. ICOS-Ig treatment drastically enhanced bacterial titers, and a large fraction of mice succumbed to the otherwise sublethal dose of infection. Thus, ICOS costimulation is crucial for protective immunity against the intracellular bacterium L. monocytogenes.

KW - Administration, Oral

KW - Animals

KW - Antibodies, Blocking

KW - Antibodies, Monoclonal

KW - Antigens, Differentiation, T-Lymphocyte

KW - CD4-CD8 Ratio

KW - CD4-Positive T-Lymphocytes

KW - CD8-Positive T-Lymphocytes

KW - Disease Susceptibility

KW - Epitopes, T-Lymphocyte

KW - Humans

KW - Inducible T-Cell Co-Stimulator Protein

KW - Injections, Intravenous

KW - Injections, Subcutaneous

KW - Interferon-gamma

KW - Listeria monocytogenes

KW - Listeriosis

KW - Lymphocyte Activation

KW - Lymphocyte Depletion

KW - Mice

KW - Mice, Inbred BALB C

KW - Rats

KW - Rats, Inbred Strains

KW - Recombinant Fusion Proteins

KW - Signal Transduction

KW - T-Lymphocyte Subsets

M3 - SCORING: Journal article

C2 - 12421962

VL - 169

SP - 5813

EP - 5817

JO - J IMMUNOL

JF - J IMMUNOL

SN - 0022-1767

IS - 10

ER -