In-depth characterization of monocyte subsets during the course of healthy pregnancy
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In-depth characterization of monocyte subsets during the course of healthy pregnancy. / Pflitsch, Caroline; Feldmann, Cai Niklaas; Richert, Laura; Hagen, Sven; Diemert, Anke; Goletzke, Janina; Hecher, Kurt; Jazbutyte, Virginija; Renné, Thomas; Arck, Petra Clara; Altfeld, Marcus; Ziegler, Susanne.
in: J REPROD IMMUNOL, Jahrgang 141, 09.2020, S. 103151.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - In-depth characterization of monocyte subsets during the course of healthy pregnancy
AU - Pflitsch, Caroline
AU - Feldmann, Cai Niklaas
AU - Richert, Laura
AU - Hagen, Sven
AU - Diemert, Anke
AU - Goletzke, Janina
AU - Hecher, Kurt
AU - Jazbutyte, Virginija
AU - Renné, Thomas
AU - Arck, Petra Clara
AU - Altfeld, Marcus
AU - Ziegler, Susanne
N1 - Copyright © 2020 Elsevier B.V. All rights reserved.
PY - 2020/9
Y1 - 2020/9
N2 - Pregnancy represents an immunological challenge for the maternal immune system. Pregnancy augments innate immune responses, and particularly monocytes contribute to maintaining the balance between pro- and anti-inflammatory immune responses required for the successful sequence of distinct immunological phases throughout pregnancy. Nonetheless, studies that focus on the heterogeneity of monocytes and analyze the alteration of monocyte subsets in a longitudinal approach throughout healthy pregnancies have remained scarce. In this study, we characterized the gradual phenotypic changes of monocyte subsets and the secretory potential of bulk monocytes in peripheral blood mononuclear cells of healthy pregnant women from a population-based prospective birth cohort study. Blood samples at predefined time points were analyzed using flow cytometry for in-depth characterization of monocyte subsets, which confirmed a shift from classical towards intermediate monocytes throughout pregnancy. Principal component analysis revealed characteristic phenotypic changes on monocyte subsets, especially on the intermediate monocyte subset, throughout pregnancy. Pregnancy-related hormones were measured in serum and β-human chorionic gonadotropin levels were significantly associated with expression of CD11b, CD116 and CCR2 on monocyte subsets. TLR4 and TLR7/8 stimulation of monocytes furthermore showed reduced polycytokine production towards the end of pregnancy. These data provide a comprehensive overview of phenotypic changes and secretory potential of monocytes in healthy pregnant women and establish a selective contribution of different monocyte subsets to healthy pregnancy. The results from this study therefore build a basis for future comparisons and evaluation of women with adverse pregnancy outcomes.
AB - Pregnancy represents an immunological challenge for the maternal immune system. Pregnancy augments innate immune responses, and particularly monocytes contribute to maintaining the balance between pro- and anti-inflammatory immune responses required for the successful sequence of distinct immunological phases throughout pregnancy. Nonetheless, studies that focus on the heterogeneity of monocytes and analyze the alteration of monocyte subsets in a longitudinal approach throughout healthy pregnancies have remained scarce. In this study, we characterized the gradual phenotypic changes of monocyte subsets and the secretory potential of bulk monocytes in peripheral blood mononuclear cells of healthy pregnant women from a population-based prospective birth cohort study. Blood samples at predefined time points were analyzed using flow cytometry for in-depth characterization of monocyte subsets, which confirmed a shift from classical towards intermediate monocytes throughout pregnancy. Principal component analysis revealed characteristic phenotypic changes on monocyte subsets, especially on the intermediate monocyte subset, throughout pregnancy. Pregnancy-related hormones were measured in serum and β-human chorionic gonadotropin levels were significantly associated with expression of CD11b, CD116 and CCR2 on monocyte subsets. TLR4 and TLR7/8 stimulation of monocytes furthermore showed reduced polycytokine production towards the end of pregnancy. These data provide a comprehensive overview of phenotypic changes and secretory potential of monocytes in healthy pregnant women and establish a selective contribution of different monocyte subsets to healthy pregnancy. The results from this study therefore build a basis for future comparisons and evaluation of women with adverse pregnancy outcomes.
U2 - 10.1016/j.jri.2020.103151
DO - 10.1016/j.jri.2020.103151
M3 - SCORING: Journal article
C2 - 32531656
VL - 141
SP - 103151
JO - J REPROD IMMUNOL
JF - J REPROD IMMUNOL
SN - 0165-0378
ER -