In vivo toxicity study of rhodamine 6G in the rat retina
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In vivo toxicity study of rhodamine 6G in the rat retina. / Thaler, Sebastian; Haritoglou, Christos; Choragiewicz, Tomasz J; Messias, Andre; Baryluk, Aneta; May, C Albrecht; Rejdak, Robert; Fiedorowicz, Michal; Zrenner, Eberhart; Schuettauf, Frank.
in: INVEST OPHTH VIS SCI, Jahrgang 49, Nr. 5, 05.2008, S. 2120-6.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - In vivo toxicity study of rhodamine 6G in the rat retina
AU - Thaler, Sebastian
AU - Haritoglou, Christos
AU - Choragiewicz, Tomasz J
AU - Messias, Andre
AU - Baryluk, Aneta
AU - May, C Albrecht
AU - Rejdak, Robert
AU - Fiedorowicz, Michal
AU - Zrenner, Eberhart
AU - Schuettauf, Frank
PY - 2008/5
Y1 - 2008/5
N2 - PURPOSE: To investigate the intraocular effect of rhodamine 6G (R6G) on retinal structures and function in an in vivo rat model and to develop an in vivo method for accurate evaluation of new dyes for intraocular surgery.METHODS: R6G in physiologic saline solution (PSS) was injected into the vitreous of adult Brown Norway rats at concentrations of 0.0002%, 0.002%, 0.02%, 0.2%, and 0.5%. Control animals received only PSS. Retinal toxicity was assessed by retinal ganglion cell (RGC) counts, light microscopy 7 days later, photopic electroretinography (ERG), and measurement of scotopic sensitivity and recovery of dark adaptation 48 hours and 7 days after intravitreous injection.RESULTS: R6G at concentrations of 0.2% and 0.5% led to a dose-dependent loss of RGC. The most significant loss occurred at 0.5%. Lower concentrations (0.0002%, 0.002%, and 0.02%) produced no statistically significant retinal ganglion cell loss. Analysis of the eyes by light microscopy showed no structural changes in the central retina, although injections of 0.5% R6G were followed by impressive degenerative changes adjacent to the injection sites. ERGs showed no effects of the highest R6G concentration on rods, kinetics of rhodopsin recovery after bleaching, or cone-driven responses.CONCLUSIONS: R6G can be safely injected in doses of up to 0.02% in rats, but has a toxic effect on retinal ganglion cells at higher concentrations. Accumulation of R6G may be a problem at higher concentrations, particularly at the injection site.
AB - PURPOSE: To investigate the intraocular effect of rhodamine 6G (R6G) on retinal structures and function in an in vivo rat model and to develop an in vivo method for accurate evaluation of new dyes for intraocular surgery.METHODS: R6G in physiologic saline solution (PSS) was injected into the vitreous of adult Brown Norway rats at concentrations of 0.0002%, 0.002%, 0.02%, 0.2%, and 0.5%. Control animals received only PSS. Retinal toxicity was assessed by retinal ganglion cell (RGC) counts, light microscopy 7 days later, photopic electroretinography (ERG), and measurement of scotopic sensitivity and recovery of dark adaptation 48 hours and 7 days after intravitreous injection.RESULTS: R6G at concentrations of 0.2% and 0.5% led to a dose-dependent loss of RGC. The most significant loss occurred at 0.5%. Lower concentrations (0.0002%, 0.002%, and 0.02%) produced no statistically significant retinal ganglion cell loss. Analysis of the eyes by light microscopy showed no structural changes in the central retina, although injections of 0.5% R6G were followed by impressive degenerative changes adjacent to the injection sites. ERGs showed no effects of the highest R6G concentration on rods, kinetics of rhodopsin recovery after bleaching, or cone-driven responses.CONCLUSIONS: R6G can be safely injected in doses of up to 0.02% in rats, but has a toxic effect on retinal ganglion cells at higher concentrations. Accumulation of R6G may be a problem at higher concentrations, particularly at the injection site.
KW - Animals
KW - Cell Count
KW - Dark Adaptation
KW - Dose-Response Relationship, Drug
KW - Electroretinography/drug effects
KW - Fluorescent Dyes/administration & dosage
KW - Male
KW - Oscillometry
KW - Rats
KW - Rats, Inbred BN
KW - Retina/drug effects
KW - Retinal Degeneration/chemically induced
KW - Retinal Ganglion Cells/drug effects
KW - Rhodamines/administration & dosage
U2 - 10.1167/iovs.07-1476
DO - 10.1167/iovs.07-1476
M3 - SCORING: Journal article
C2 - 18436844
VL - 49
SP - 2120
EP - 2126
JO - INVEST OPHTH VIS SCI
JF - INVEST OPHTH VIS SCI
SN - 0146-0404
IS - 5
ER -