In vivo imaging of embryonic stem cells reveals patterns of survival and immune rejection following transplantation
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In vivo imaging of embryonic stem cells reveals patterns of survival and immune rejection following transplantation. / Swijnenburg, Rutger-Jan; Schrepfer, Sonja; Cao, Feng; Pearl, Jeremy I; Xie, Xiaoyan; Connolly, Andrew J; Robbins, Robert C; Wu, Joseph C.
in: STEM CELLS DEV, Jahrgang 17, Nr. 6, 12.2008, S. 1023-1029.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - In vivo imaging of embryonic stem cells reveals patterns of survival and immune rejection following transplantation
AU - Swijnenburg, Rutger-Jan
AU - Schrepfer, Sonja
AU - Cao, Feng
AU - Pearl, Jeremy I
AU - Xie, Xiaoyan
AU - Connolly, Andrew J
AU - Robbins, Robert C
AU - Wu, Joseph C
PY - 2008/12
Y1 - 2008/12
N2 - Embryonic stem cell (ESC)-based transplantation is considered a promising novel therapy for a variety of diseases. This is bolstered by the suggested immune-privileged properties of ESCs. In this study, we used in vivo bioluminescent imaging (BLI) to non-invasively track the fate of transplanted murine ESCs (mESCs), which are stably transduced with a double fusion reporter gene consisting of firefly luciferase (FLuc) and enhanced green fluorescent protein (eGFP). Following syngeneic intramuscular transplantation of 1 x 10(6) mESCs, the cells survived and differentiated into teratomas. In contrast, allogeneic mESC transplants were infiltrated by a variety of inflammatory cells, leading to rejection within 28 days. Acceleration of rejection was observed when mESCs were allotransplanted following prior sensitization of the host. Finally, we demonstrate that the mESC derivatives were more rapidly rejected compared to undifferentiated mESCs. These data show that mESCs do not retain immune-privileged properties in vivo and are subject to immunological rejection as assessed by novel molecular imaging approaches.
AB - Embryonic stem cell (ESC)-based transplantation is considered a promising novel therapy for a variety of diseases. This is bolstered by the suggested immune-privileged properties of ESCs. In this study, we used in vivo bioluminescent imaging (BLI) to non-invasively track the fate of transplanted murine ESCs (mESCs), which are stably transduced with a double fusion reporter gene consisting of firefly luciferase (FLuc) and enhanced green fluorescent protein (eGFP). Following syngeneic intramuscular transplantation of 1 x 10(6) mESCs, the cells survived and differentiated into teratomas. In contrast, allogeneic mESC transplants were infiltrated by a variety of inflammatory cells, leading to rejection within 28 days. Acceleration of rejection was observed when mESCs were allotransplanted following prior sensitization of the host. Finally, we demonstrate that the mESC derivatives were more rapidly rejected compared to undifferentiated mESCs. These data show that mESCs do not retain immune-privileged properties in vivo and are subject to immunological rejection as assessed by novel molecular imaging approaches.
KW - Animals
KW - Cell Survival/immunology
KW - Diagnostic Imaging
KW - Embryonic Stem Cells/immunology
KW - Genes, Reporter/immunology
KW - Graft Rejection/genetics
KW - Green Fluorescent Proteins/biosynthesis
KW - Luciferases, Firefly/biosynthesis
KW - Luminescent Measurements
KW - Mice
KW - Mice, Inbred BALB C
KW - Recombinant Fusion Proteins/biosynthesis
KW - Stem Cell Transplantation
KW - Teratoma/genetics
KW - Transplantation, Homologous
KW - Transplantation, Isogeneic
U2 - 10.1089/scd.2008.0091
DO - 10.1089/scd.2008.0091
M3 - SCORING: Journal article
C2 - 18491958
VL - 17
SP - 1023
EP - 1029
JO - STEM CELLS DEV
JF - STEM CELLS DEV
SN - 1547-3287
IS - 6
ER -
