In vitro sulfation of N-acetyllactosaminide by soluble recombinant human beta-Gal-3'-sulfotransferase

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In vitro sulfation of N-acetyllactosaminide by soluble recombinant human beta-Gal-3'-sulfotransferase. / Greimel, P; Jabs, Sabrina; Storch, Stephan; Cherif, Slim; Honke, Koichi; Braulke, Thomas; Thiem, Joachim.

in: CARBOHYD RES, Jahrgang 341, Nr. 7, 22.05.2006, S. 918-24.

Publikationen: SCORING: Beitrag in Fachzeitschrift/ZeitungSCORING: ZeitschriftenaufsatzForschungBegutachtung

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@article{6e814d9b75ab48cea13c8bbfc1c3cf1b,
title = "In vitro sulfation of N-acetyllactosaminide by soluble recombinant human beta-Gal-3'-sulfotransferase",
abstract = "Membrane-bound beta-Gal-3'-sulfotransferase (GP3ST) was expressed and used for in vitro sulfation of Tamm-Horsfall glycoprotein. Further, the regioselective transfer of sulfate to an N-acetyllactosamine derivative could be realised with soluble chimeric GP3ST, also in combination with Lac transglycosylation by means of beta-galactosidase. Two alternative straightforward chemical syntheses for the target compound could be elaborated.",
keywords = "Animals, Binding Sites, Cell Line, Cricetinae, Glycoside Hydrolases, Glycosylation, Humans, Mucoproteins, Oligosaccharides, Protein Binding, Protein Conformation, Protein Structure, Tertiary, Recombinant Fusion Proteins, Solubility, Staphylococcal Protein A, Sulfates, Sulfotransferases, Uromodulin",
author = "P Greimel and Sabrina Jabs and Stephan Storch and Slim Cherif and Koichi Honke and Thomas Braulke and Joachim Thiem",
year = "2006",
month = may,
day = "22",
doi = "10.1016/j.carres.2006.02.006",
language = "English",
volume = "341",
pages = "918--24",
journal = "CARBOHYD RES",
issn = "0008-6215",
publisher = "Elsevier BV",
number = "7",

}

RIS

TY - JOUR

T1 - In vitro sulfation of N-acetyllactosaminide by soluble recombinant human beta-Gal-3'-sulfotransferase

AU - Greimel, P

AU - Jabs, Sabrina

AU - Storch, Stephan

AU - Cherif, Slim

AU - Honke, Koichi

AU - Braulke, Thomas

AU - Thiem, Joachim

PY - 2006/5/22

Y1 - 2006/5/22

N2 - Membrane-bound beta-Gal-3'-sulfotransferase (GP3ST) was expressed and used for in vitro sulfation of Tamm-Horsfall glycoprotein. Further, the regioselective transfer of sulfate to an N-acetyllactosamine derivative could be realised with soluble chimeric GP3ST, also in combination with Lac transglycosylation by means of beta-galactosidase. Two alternative straightforward chemical syntheses for the target compound could be elaborated.

AB - Membrane-bound beta-Gal-3'-sulfotransferase (GP3ST) was expressed and used for in vitro sulfation of Tamm-Horsfall glycoprotein. Further, the regioselective transfer of sulfate to an N-acetyllactosamine derivative could be realised with soluble chimeric GP3ST, also in combination with Lac transglycosylation by means of beta-galactosidase. Two alternative straightforward chemical syntheses for the target compound could be elaborated.

KW - Animals

KW - Binding Sites

KW - Cell Line

KW - Cricetinae

KW - Glycoside Hydrolases

KW - Glycosylation

KW - Humans

KW - Mucoproteins

KW - Oligosaccharides

KW - Protein Binding

KW - Protein Conformation

KW - Protein Structure, Tertiary

KW - Recombinant Fusion Proteins

KW - Solubility

KW - Staphylococcal Protein A

KW - Sulfates

KW - Sulfotransferases

KW - Uromodulin

U2 - 10.1016/j.carres.2006.02.006

DO - 10.1016/j.carres.2006.02.006

M3 - SCORING: Journal article

C2 - 16516177

VL - 341

SP - 918

EP - 924

JO - CARBOHYD RES

JF - CARBOHYD RES

SN - 0008-6215

IS - 7

ER -