In vitro sulfation of N-acetyllactosaminide by soluble recombinant human beta-Gal-3'-sulfotransferase
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In vitro sulfation of N-acetyllactosaminide by soluble recombinant human beta-Gal-3'-sulfotransferase. / Greimel, P; Jabs, Sabrina; Storch, Stephan; Cherif, Slim; Honke, Koichi; Braulke, Thomas; Thiem, Joachim.
in: CARBOHYD RES, Jahrgang 341, Nr. 7, 22.05.2006, S. 918-24.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - In vitro sulfation of N-acetyllactosaminide by soluble recombinant human beta-Gal-3'-sulfotransferase
AU - Greimel, P
AU - Jabs, Sabrina
AU - Storch, Stephan
AU - Cherif, Slim
AU - Honke, Koichi
AU - Braulke, Thomas
AU - Thiem, Joachim
PY - 2006/5/22
Y1 - 2006/5/22
N2 - Membrane-bound beta-Gal-3'-sulfotransferase (GP3ST) was expressed and used for in vitro sulfation of Tamm-Horsfall glycoprotein. Further, the regioselective transfer of sulfate to an N-acetyllactosamine derivative could be realised with soluble chimeric GP3ST, also in combination with Lac transglycosylation by means of beta-galactosidase. Two alternative straightforward chemical syntheses for the target compound could be elaborated.
AB - Membrane-bound beta-Gal-3'-sulfotransferase (GP3ST) was expressed and used for in vitro sulfation of Tamm-Horsfall glycoprotein. Further, the regioselective transfer of sulfate to an N-acetyllactosamine derivative could be realised with soluble chimeric GP3ST, also in combination with Lac transglycosylation by means of beta-galactosidase. Two alternative straightforward chemical syntheses for the target compound could be elaborated.
KW - Animals
KW - Binding Sites
KW - Cell Line
KW - Cricetinae
KW - Glycoside Hydrolases
KW - Glycosylation
KW - Humans
KW - Mucoproteins
KW - Oligosaccharides
KW - Protein Binding
KW - Protein Conformation
KW - Protein Structure, Tertiary
KW - Recombinant Fusion Proteins
KW - Solubility
KW - Staphylococcal Protein A
KW - Sulfates
KW - Sulfotransferases
KW - Uromodulin
U2 - 10.1016/j.carres.2006.02.006
DO - 10.1016/j.carres.2006.02.006
M3 - SCORING: Journal article
C2 - 16516177
VL - 341
SP - 918
EP - 924
JO - CARBOHYD RES
JF - CARBOHYD RES
SN - 0008-6215
IS - 7
ER -