In vitro and in vivo comparison of a tailored magnetic particle imaging blood pool tracer with resovist

TY - JOUR

T1 - In vitro and in vivo comparison of a tailored magnetic particle imaging blood pool tracer with resovist

AU - Kaul, Michael Gerhard

AU - Mummert, Tobias

AU - Jung, Caroline

AU - Salamon, Johannes

AU - Khandhar, Amit

AU - Ferguson, R Matthew

AU - Kemp, Scott

AU - Ittrich, Harald

AU - Krishnan, Kannan

AU - Adam, Gerhard

AU - Knopp, Tobias

N1 - Copyright 2017 Institute of Physics and Engineering in Medicine.

PY - 2017/5/7

Y1 - 2017/5/7

N2 - Optimizing tracers for individual imaging techniques is an active field of research. The purpose of this study was to perform in vitro and in vivo magnetic particle imaging (MPI) measurements using a new monodisperse and size-optimized tracer, LS-008, and to compare it with the performance of Resovist, the standard MPI tracer. In vitro measurements were performed in concerns of concentration and amount of tracer in a phantom. In vivo studies were carried out in healthy FVB mice. The first group (n=3) received 60µL LS-008 (87mM) and the second (n=3) diluted Resovist of the same concentration and volume. Tracer injections were performed with a syringe pump during a dynamic MPI scan. For anatomic referencing MRI was applied before the performance of the MPI measurements. Summing up the results the in vitro experiments showed for LS-008 a better sensitivity and spatial resolution than Resovist. In vivo both tracers can visualize the propagation of the bolus through the inferior vena cava. MPI with LS-008 did show less temporal fluctuation artifacts and the pulsation of blood due to respiratory and cardiac cycle was detectable. With LS-008 the aorta was distinguishable from the caval vein while with Resovist this failed. A liver vessel and a vessel structure leading cranially could only be observed with LS-008 and not with Resovist. Beside these structural advantages both tracers showed very different blood half-life. For LS-008 we found 88 minutes. Resovist did show a fast liver accumulation and a half-life of 13 minutes. With LS-008 the perfusion fraction in liver and kidney was measureable. MPI can be significantly improved by applying more effective tracers. LS-008 shows a clear improvement concerning the delineation while resolving a larger number of vessels in comparison to Resovist. Therefore, in aspects of quality and quantity LS-008 is clearly favorable for angiographic and perfusion studies.

AB - Optimizing tracers for individual imaging techniques is an active field of research. The purpose of this study was to perform in vitro and in vivo magnetic particle imaging (MPI) measurements using a new monodisperse and size-optimized tracer, LS-008, and to compare it with the performance of Resovist, the standard MPI tracer. In vitro measurements were performed in concerns of concentration and amount of tracer in a phantom. In vivo studies were carried out in healthy FVB mice. The first group (n=3) received 60µL LS-008 (87mM) and the second (n=3) diluted Resovist of the same concentration and volume. Tracer injections were performed with a syringe pump during a dynamic MPI scan. For anatomic referencing MRI was applied before the performance of the MPI measurements. Summing up the results the in vitro experiments showed for LS-008 a better sensitivity and spatial resolution than Resovist. In vivo both tracers can visualize the propagation of the bolus through the inferior vena cava. MPI with LS-008 did show less temporal fluctuation artifacts and the pulsation of blood due to respiratory and cardiac cycle was detectable. With LS-008 the aorta was distinguishable from the caval vein while with Resovist this failed. A liver vessel and a vessel structure leading cranially could only be observed with LS-008 and not with Resovist. Beside these structural advantages both tracers showed very different blood half-life. For LS-008 we found 88 minutes. Resovist did show a fast liver accumulation and a half-life of 13 minutes. With LS-008 the perfusion fraction in liver and kidney was measureable. MPI can be significantly improved by applying more effective tracers. LS-008 shows a clear improvement concerning the delineation while resolving a larger number of vessels in comparison to Resovist. Therefore, in aspects of quality and quantity LS-008 is clearly favorable for angiographic and perfusion studies.

U2 - 10.1088/1361-6560/aa5780

DO - 10.1088/1361-6560/aa5780

M3 - SCORING: Journal article

C2 - 28060771

VL - 62

SP - 3454

EP - 3469

JO - PHYS MED BIOL

JF - PHYS MED BIOL

SN - 0031-9155

IS - 9

ER -