In vitro activity of polyoxin D and nikkomycin Z against Encephalitozoon cuniculi.
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In vitro activity of polyoxin D and nikkomycin Z against Encephalitozoon cuniculi. / Sobottka, Ingo; Bartscht, Katrin; Schäfer, Peter; Weitzel, Thomas; Schottelius, Justus; Kock, Nico; Laufs, Rainer.
in: PARASITOL RES, Jahrgang 88, Nr. 5, 5, 2002, S. 451-453.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - In vitro activity of polyoxin D and nikkomycin Z against Encephalitozoon cuniculi.
AU - Sobottka, Ingo
AU - Bartscht, Katrin
AU - Schäfer, Peter
AU - Weitzel, Thomas
AU - Schottelius, Justus
AU - Kock, Nico
AU - Laufs, Rainer
PY - 2002
Y1 - 2002
N2 - Microsporidia of the genus Encephalitozoon are emerging protozoal agents that mainly infect immunocompromised patients with AIDS. At present, disseminated infections with members of the genus Encephalitozoon can only be successfully treated with albendazole. As chitin is a basic component of the microsporidian spore. we evaluated, in vitro, the susceptibility of a human-derived strain of Encephalitozoon cuniculi to polyoxin D and nikkomycin Z, which are known competitive inhibitors of chitin synthetase enzymes. Using an in vitro assay, polyoxin D at 1, 10 and 100 microg/ml significantly reduced the number of parasitic foci on days 6, 9, and 15 post-infection. However, nikkomycin Z revealed a marked but lower reduction in the number of parasitic foci than polyoxin D. A significant reduction of parasitic foci was achieved for nikkomycin Z at 10 and 100 microg/ml up to day 9 post-infection. Polyoxin D was approximately tenfold more effective in our in vitro assay than nikkomycin Z.
AB - Microsporidia of the genus Encephalitozoon are emerging protozoal agents that mainly infect immunocompromised patients with AIDS. At present, disseminated infections with members of the genus Encephalitozoon can only be successfully treated with albendazole. As chitin is a basic component of the microsporidian spore. we evaluated, in vitro, the susceptibility of a human-derived strain of Encephalitozoon cuniculi to polyoxin D and nikkomycin Z, which are known competitive inhibitors of chitin synthetase enzymes. Using an in vitro assay, polyoxin D at 1, 10 and 100 microg/ml significantly reduced the number of parasitic foci on days 6, 9, and 15 post-infection. However, nikkomycin Z revealed a marked but lower reduction in the number of parasitic foci than polyoxin D. A significant reduction of parasitic foci was achieved for nikkomycin Z at 10 and 100 microg/ml up to day 9 post-infection. Polyoxin D was approximately tenfold more effective in our in vitro assay than nikkomycin Z.
M3 - SCORING: Zeitschriftenaufsatz
VL - 88
SP - 451
EP - 453
JO - PARASITOL RES
JF - PARASITOL RES
SN - 0932-0113
IS - 5
M1 - 5
ER -
