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Improved methods for marking active neuron populations

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Improved methods for marking active neuron populations. / Moeyaert, Benjamien; Holt, Graham; Madangopal, Rajtarun; Perez-Alvarez, Alberto; Fearey, Brenna C; Trojanowski, Nicholas F; Ledderose, Julia; Zolnik, Timothy A; Das, Aniruddha; Patel, Davina; Brown, Timothy A; Sachdev, Robert N S; Eickholt, Britta J; Larkum, Matthew E; Turrigiano, Gina G; Dana, Hod; Gee, Christine E; Oertner, Thomas G; Hope, Bruce T; Schreiter, Eric R.

in: NAT COMMUN, Jahrgang 9, Nr. 1, 25.10.2018, S. 4440.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Moeyaert, B, Holt, G, Madangopal, R, Perez-Alvarez, A, Fearey, BC, Trojanowski, NF, Ledderose, J, Zolnik, TA, Das, A, Patel, D, Brown, TA, Sachdev, RNS, Eickholt, BJ, Larkum, ME, Turrigiano, GG, Dana, H, Gee, CE, Oertner, TG, Hope, BT & Schreiter, ER 2018, 'Improved methods for marking active neuron populations', NAT COMMUN, Jg. 9, Nr. 1, S. 4440. https://doi.org/10.1038/s41467-018-06935-2

APA

Moeyaert, B., Holt, G., Madangopal, R., Perez-Alvarez, A., Fearey, B. C., Trojanowski, N. F., Ledderose, J., Zolnik, T. A., Das, A., Patel, D., Brown, T. A., Sachdev, R. N. S., Eickholt, B. J., Larkum, M. E., Turrigiano, G. G., Dana, H., Gee, C. E., Oertner, T. G., Hope, B. T., & Schreiter, E. R. (2018). Improved methods for marking active neuron populations. NAT COMMUN, 9(1), 4440. https://doi.org/10.1038/s41467-018-06935-2

Vancouver

Moeyaert B, Holt G, Madangopal R, Perez-Alvarez A, Fearey BC, Trojanowski NF et al. Improved methods for marking active neuron populations. NAT COMMUN. 2018 Okt 25;9(1):4440. https://doi.org/10.1038/s41467-018-06935-2

Bibtex

@article{f5ae34a1f2a04146a7da8077257e2a29,
title = "Improved methods for marking active neuron populations",
abstract = "Marking functionally distinct neuronal ensembles with high spatiotemporal resolution is a key challenge in systems neuroscience. We recently introduced CaMPARI, an engineered fluorescent protein whose green-to-red photoconversion depends on simultaneous light exposure and elevated calcium, which enabled marking active neuronal populations with single-cell and subsecond resolution. However, CaMPARI (CaMPARI1) has several drawbacks, including background photoconversion in low calcium, slow kinetics and reduced fluorescence after chemical fixation. In this work, we develop CaMPARI2, an improved sensor with brighter green and red fluorescence, faster calcium unbinding kinetics and decreased photoconversion in low calcium conditions. We demonstrate the improved performance of CaMPARI2 in mammalian neurons and in vivo in larval zebrafish brain and mouse visual cortex. Additionally, we herein develop an immunohistochemical detection method for specific labeling of the photoconverted red form of CaMPARI. The anti-CaMPARI-red antibody provides strong labeling that is selective for photoconverted CaMPARI in activated neurons in rodent brain tissue.",
keywords = "Journal Article, Research Support, Non-U.S. Gov't",
author = "Benjamien Moeyaert and Graham Holt and Rajtarun Madangopal and Alberto Perez-Alvarez and Fearey, {Brenna C} and Trojanowski, {Nicholas F} and Julia Ledderose and Zolnik, {Timothy A} and Aniruddha Das and Davina Patel and Brown, {Timothy A} and Sachdev, {Robert N S} and Eickholt, {Britta J} and Larkum, {Matthew E} and Turrigiano, {Gina G} and Hod Dana and Gee, {Christine E} and Oertner, {Thomas G} and Hope, {Bruce T} and Schreiter, {Eric R}",
year = "2018",
month = oct,
day = "25",
doi = "10.1038/s41467-018-06935-2",
language = "English",
volume = "9",
pages = "4440",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Improved methods for marking active neuron populations

AU - Moeyaert, Benjamien

AU - Holt, Graham

AU - Madangopal, Rajtarun

AU - Perez-Alvarez, Alberto

AU - Fearey, Brenna C

AU - Trojanowski, Nicholas F

AU - Ledderose, Julia

AU - Zolnik, Timothy A

AU - Das, Aniruddha

AU - Patel, Davina

AU - Brown, Timothy A

AU - Sachdev, Robert N S

AU - Eickholt, Britta J

AU - Larkum, Matthew E

AU - Turrigiano, Gina G

AU - Dana, Hod

AU - Gee, Christine E

AU - Oertner, Thomas G

AU - Hope, Bruce T

AU - Schreiter, Eric R

PY - 2018/10/25

Y1 - 2018/10/25

N2 - Marking functionally distinct neuronal ensembles with high spatiotemporal resolution is a key challenge in systems neuroscience. We recently introduced CaMPARI, an engineered fluorescent protein whose green-to-red photoconversion depends on simultaneous light exposure and elevated calcium, which enabled marking active neuronal populations with single-cell and subsecond resolution. However, CaMPARI (CaMPARI1) has several drawbacks, including background photoconversion in low calcium, slow kinetics and reduced fluorescence after chemical fixation. In this work, we develop CaMPARI2, an improved sensor with brighter green and red fluorescence, faster calcium unbinding kinetics and decreased photoconversion in low calcium conditions. We demonstrate the improved performance of CaMPARI2 in mammalian neurons and in vivo in larval zebrafish brain and mouse visual cortex. Additionally, we herein develop an immunohistochemical detection method for specific labeling of the photoconverted red form of CaMPARI. The anti-CaMPARI-red antibody provides strong labeling that is selective for photoconverted CaMPARI in activated neurons in rodent brain tissue.

AB - Marking functionally distinct neuronal ensembles with high spatiotemporal resolution is a key challenge in systems neuroscience. We recently introduced CaMPARI, an engineered fluorescent protein whose green-to-red photoconversion depends on simultaneous light exposure and elevated calcium, which enabled marking active neuronal populations with single-cell and subsecond resolution. However, CaMPARI (CaMPARI1) has several drawbacks, including background photoconversion in low calcium, slow kinetics and reduced fluorescence after chemical fixation. In this work, we develop CaMPARI2, an improved sensor with brighter green and red fluorescence, faster calcium unbinding kinetics and decreased photoconversion in low calcium conditions. We demonstrate the improved performance of CaMPARI2 in mammalian neurons and in vivo in larval zebrafish brain and mouse visual cortex. Additionally, we herein develop an immunohistochemical detection method for specific labeling of the photoconverted red form of CaMPARI. The anti-CaMPARI-red antibody provides strong labeling that is selective for photoconverted CaMPARI in activated neurons in rodent brain tissue.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/s41467-018-06935-2

DO - 10.1038/s41467-018-06935-2

M3 - SCORING: Journal article

C2 - 30361563

VL - 9

SP - 4440

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

ER -