Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12
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Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12. / Jung, Ole; Smeets, Ralf; Hartjen, Philip; Schnettler, Reinhard; Feyerabend, Frank; Klein, Martin; Wegner, Nils; Walther, Frank; Stangier, Dominic; Henningsen, Anders; Rendenbach, Carsten; Heiland, Max; Barbeck, Mike; Kopp, Alexander.
in: INT J MOL SCI, Jahrgang 20, Nr. 2, 10.01.2019.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Improved In Vitro Test Procedure for Full Assessment of the Cytocompatibility of Degradable Magnesium Based on ISO 10993-5/-12
AU - Jung, Ole
AU - Smeets, Ralf
AU - Hartjen, Philip
AU - Schnettler, Reinhard
AU - Feyerabend, Frank
AU - Klein, Martin
AU - Wegner, Nils
AU - Walther, Frank
AU - Stangier, Dominic
AU - Henningsen, Anders
AU - Rendenbach, Carsten
AU - Heiland, Max
AU - Barbeck, Mike
AU - Kopp, Alexander
PY - 2019/1/10
Y1 - 2019/1/10
N2 - Magnesium (Mg)-based biomaterials are promising candidates for bone and tissue regeneration. Alloying and surface modifications provide effective strategies for optimizing and tailoring their degradation kinetics. Nevertheless, biocompatibility analyses of Mg-based materials are challenging due to its special degradation mechanism with continuous hydrogen release. In this context, the hydrogen release and the related (micro-) milieu conditions pretend to strictly follow in vitro standards based on ISO 10993-5/-12. Thus, special adaptions for the testing of Mg materials are necessary, which have been described in a previous study from our group. Based on these adaptions, further developments of a test procedure allowing rapid and effective in vitro cytocompatibility analyses of Mg-based materials based on ISO 10993-5/-12 are necessary. The following study introduces a new two-step test scheme for rapid and effective testing of Mg. Specimens with different surface characteristics were produced by means of plasma electrolytic oxidation (PEO) using silicate-based and phosphate-based electrolytes. The test samples were evaluated for corrosion behavior, cytocompatibility and their mechanical and osteogenic properties. Thereby, two PEO ceramics could be identified for further in vivo evaluations.
AB - Magnesium (Mg)-based biomaterials are promising candidates for bone and tissue regeneration. Alloying and surface modifications provide effective strategies for optimizing and tailoring their degradation kinetics. Nevertheless, biocompatibility analyses of Mg-based materials are challenging due to its special degradation mechanism with continuous hydrogen release. In this context, the hydrogen release and the related (micro-) milieu conditions pretend to strictly follow in vitro standards based on ISO 10993-5/-12. Thus, special adaptions for the testing of Mg materials are necessary, which have been described in a previous study from our group. Based on these adaptions, further developments of a test procedure allowing rapid and effective in vitro cytocompatibility analyses of Mg-based materials based on ISO 10993-5/-12 are necessary. The following study introduces a new two-step test scheme for rapid and effective testing of Mg. Specimens with different surface characteristics were produced by means of plasma electrolytic oxidation (PEO) using silicate-based and phosphate-based electrolytes. The test samples were evaluated for corrosion behavior, cytocompatibility and their mechanical and osteogenic properties. Thereby, two PEO ceramics could be identified for further in vivo evaluations.
KW - Journal Article
U2 - 10.3390/ijms20020255
DO - 10.3390/ijms20020255
M3 - SCORING: Journal article
C2 - 30634646
VL - 20
JO - INT J MOL SCI
JF - INT J MOL SCI
SN - 1661-6596
IS - 2
ER -
