Impaired stria vascularis integrity upon loss of E-cadherin in basal cells.
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Impaired stria vascularis integrity upon loss of E-cadherin in basal cells. / Trowe, Mark-Oliver; Maier, Hannes; Petry, Marianne; Schweizer, Michaela; Schuster-Gossler, Karin; Kispert, Andreas.
in: DEV BIOL, Jahrgang 359, Nr. 1, 1, 2011, S. 95-107.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Impaired stria vascularis integrity upon loss of E-cadherin in basal cells.
AU - Trowe, Mark-Oliver
AU - Maier, Hannes
AU - Petry, Marianne
AU - Schweizer, Michaela
AU - Schuster-Gossler, Karin
AU - Kispert, Andreas
PY - 2011
Y1 - 2011
N2 - In the cochlea, sensory transduction depends on the endocochlear potential (EP) and the unique composition of the endolymph, both of which are maintained by a highly specialized epithelium at the cochlear lateral wall, the stria vascularis. The generation of the EP by the stria vascularis, in turn, relies on the insulation of an intrastrial extracellular compartment by epithelial basal cells. Despite the physiological importance of basal cells, their cellular origin and the molecular pathways that lead to their differentiation are unclear. Here, we show by genetic lineage tracing in the mouse that basal cells exclusively derive from the otic mesenchyme. Conditional deletion of E-cadherin in the otic mesenchyme and its descendants does not abrogate the transition from mesenchymal precursors to epithelial basal cells. Rather, dedifferentiation of intermediate cells, altered morphology of basal and marginal cells and hearing impairment due to decreased EP in E-cadherin mutant mice demonstrate an essential role of E-cadherin in terminal basal cell differentiation and their interaction with other strial cell types to establish and maintain the functional architecture of the stria vascularis.
AB - In the cochlea, sensory transduction depends on the endocochlear potential (EP) and the unique composition of the endolymph, both of which are maintained by a highly specialized epithelium at the cochlear lateral wall, the stria vascularis. The generation of the EP by the stria vascularis, in turn, relies on the insulation of an intrastrial extracellular compartment by epithelial basal cells. Despite the physiological importance of basal cells, their cellular origin and the molecular pathways that lead to their differentiation are unclear. Here, we show by genetic lineage tracing in the mouse that basal cells exclusively derive from the otic mesenchyme. Conditional deletion of E-cadherin in the otic mesenchyme and its descendants does not abrogate the transition from mesenchymal precursors to epithelial basal cells. Rather, dedifferentiation of intermediate cells, altered morphology of basal and marginal cells and hearing impairment due to decreased EP in E-cadherin mutant mice demonstrate an essential role of E-cadherin in terminal basal cell differentiation and their interaction with other strial cell types to establish and maintain the functional architecture of the stria vascularis.
KW - Animals
KW - Male
KW - Female
KW - Immunohistochemistry
KW - Mice
KW - Base Sequence
KW - Mice, Transgenic
KW - Polymerase Chain Reaction
KW - DNA Primers
KW - Cadherins/genetics
KW - Stria Vascularis/cytology/physiology
KW - Animals
KW - Male
KW - Female
KW - Immunohistochemistry
KW - Mice
KW - Base Sequence
KW - Mice, Transgenic
KW - Polymerase Chain Reaction
KW - DNA Primers
KW - Cadherins/genetics
KW - Stria Vascularis/cytology/physiology
M3 - SCORING: Journal article
VL - 359
SP - 95
EP - 107
JO - DEV BIOL
JF - DEV BIOL
SN - 0012-1606
IS - 1
M1 - 1
ER -
