Impaired Fear Extinction Due to a Deficit in Ca(2+) Influx Through L-Type Voltage-Gated Ca(2+) Channels in Mice Deficient for Tenascin-C
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Impaired Fear Extinction Due to a Deficit in Ca(2+) Influx Through L-Type Voltage-Gated Ca(2+) Channels in Mice Deficient for Tenascin-C. / Morellini, Fabio; Malyshev, Aleksey; Volgushev, Maxim; Chistiakova, Marina; Papashvili, Giorgi; Fellini, Laetitia; Kleene, Ralf; Schachner, Melitta; Dityatev, Alexander.
in: FRONT INTEGR NEUROSC, Jahrgang 11, 02.08.2017, S. 16.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Impaired Fear Extinction Due to a Deficit in Ca(2+) Influx Through L-Type Voltage-Gated Ca(2+) Channels in Mice Deficient for Tenascin-C
AU - Morellini, Fabio
AU - Malyshev, Aleksey
AU - Volgushev, Maxim
AU - Chistiakova, Marina
AU - Papashvili, Giorgi
AU - Fellini, Laetitia
AU - Kleene, Ralf
AU - Schachner, Melitta
AU - Dityatev, Alexander
PY - 2017/8/2
Y1 - 2017/8/2
N2 - Mice deficient in the extracellular matrix glycoprotein tenascin-C (TNC(-/-)) express a deficit in specific forms of hippocampal synaptic plasticity, which involve the L-type voltage-gated Ca(2+) channels (L-VGCCs). The mechanisms underlying this deficit and its functional implications for learning and memory have not been investigated. In line with previous findings, we report on impairment in theta-burst stimulation (TBS)-induced long-term potentiation (LTP) in TNC(-/-) mice in the CA1 hippocampal region and its rescue by the L-VGCC activator Bay K-8644. We further found that the overall pattern of L-VGCC expression in the hippocampus in TNC(-/-) mice was normal, but Western blot analysis results uncovered upregulated expression of the Cav1.2 and Cav1.3 α-subunits of L-VGCCs. However, these L-VGCCs were not fully functional in TNC(-/-) mice, as demonstrated by Ca(2+) imaging, which revealed a reduction of nifedipine-sensitive Ca(2+) transients in CA1 pyramidal neurons. TNC(-/-) mice showed normal learning and memory in the contextual fear conditioning paradigm but impaired extinction of conditioned fear responses. Systemic injection of the L-VGCC blockers nifedipine and diltiazem into wild-type mice mimicked the impairment of fear extinction observed in TNC(-/-) mice. The deficiency in TNC(-/-) mice substantially occluded the effects of these drugs. Our results suggest that TNC-mediated modulation of L-VGCC activity is essential for fear extinction.
AB - Mice deficient in the extracellular matrix glycoprotein tenascin-C (TNC(-/-)) express a deficit in specific forms of hippocampal synaptic plasticity, which involve the L-type voltage-gated Ca(2+) channels (L-VGCCs). The mechanisms underlying this deficit and its functional implications for learning and memory have not been investigated. In line with previous findings, we report on impairment in theta-burst stimulation (TBS)-induced long-term potentiation (LTP) in TNC(-/-) mice in the CA1 hippocampal region and its rescue by the L-VGCC activator Bay K-8644. We further found that the overall pattern of L-VGCC expression in the hippocampus in TNC(-/-) mice was normal, but Western blot analysis results uncovered upregulated expression of the Cav1.2 and Cav1.3 α-subunits of L-VGCCs. However, these L-VGCCs were not fully functional in TNC(-/-) mice, as demonstrated by Ca(2+) imaging, which revealed a reduction of nifedipine-sensitive Ca(2+) transients in CA1 pyramidal neurons. TNC(-/-) mice showed normal learning and memory in the contextual fear conditioning paradigm but impaired extinction of conditioned fear responses. Systemic injection of the L-VGCC blockers nifedipine and diltiazem into wild-type mice mimicked the impairment of fear extinction observed in TNC(-/-) mice. The deficiency in TNC(-/-) mice substantially occluded the effects of these drugs. Our results suggest that TNC-mediated modulation of L-VGCC activity is essential for fear extinction.
KW - Journal Article
U2 - 10.3389/fnint.2017.00016
DO - 10.3389/fnint.2017.00016
M3 - SCORING: Journal article
C2 - 28824389
VL - 11
SP - 16
JO - FRONT INTEGR NEUROSC
JF - FRONT INTEGR NEUROSC
SN - 1662-5145
ER -