PortalPublikationenImpact of Intracardiac Neurons on Cardiac Electrophysiology ...

Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System

Standard

Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System. / Jungen, Christiane; Scherschel, Katharina; Bork, Nadja I; Kuklik, Pawel; Eickholt, Christian; Kniep, Helge; Klatt, Niklas; Willems, Stephan; Nikolaev, Viacheslav O; Meyer, Christian.

in: JOVE-J VIS EXP, Nr. 135, 22.05.2018.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Jungen, C, Scherschel, K, Bork, NI, Kuklik, P, Eickholt, C, Kniep, H, Klatt, N, Willems, S, Nikolaev, VO & Meyer, C 2018, 'Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System', JOVE-J VIS EXP, Nr. 135. https://doi.org/10.3791/57617

APA

Jungen, C., Scherschel, K., Bork, N. I., Kuklik, P., Eickholt, C., Kniep, H., Klatt, N., Willems, S., Nikolaev, V. O., & Meyer, C. (2018). Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System. JOVE-J VIS EXP, (135). https://doi.org/10.3791/57617

Vancouver

Jungen C, Scherschel K, Bork NI, Kuklik P, Eickholt C, Kniep H et al. Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System. JOVE-J VIS EXP. 2018 Mai 22;(135). https://doi.org/10.3791/57617

Bibtex

@article{2abae2291fd5409da347043d885c95f6,
title = "Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System",
abstract = "Since its invention in the late 19th century, the Langendorff ex vivo heart perfusion system continues to be a relevant tool for studying a broad spectrum of physiological, biochemical, morphological, and pharmacological parameters in centrally denervated hearts. Here, we describe a setup for the modulation of the intracardiac autonomic nervous system and the assessment of its influence on basic electrophysiology, arrhythmogenesis, and cyclic adenosine monophosphate (cAMP) dynamics. The intracardiac autonomic nervous system is modulated by the mechanical dissection of atrial fat pads-in which murine ganglia are located mainly-or by the usage of global as well as targeted pharmacological interventions. An octapolar electrophysiological catheter is introduced into the right atrium and the right ventricle, and epicardial-placed multi-electrode arrays (MEA) for high-resolution mapping are used to determine cardiac electrophysiology and arrhythmogenesis. F{\"o}rster resonance energy transfer (FRET) imaging is performed for the real-time monitoring of cAMP levels in different cardiac regions. Neuromorphology is studied by means of antibody-based staining of whole hearts using neuronal markers to guide the identification and modulation of specific targets of the intracardiac autonomic nervous system in the performed studies. The ex vivo Langendorff setup allows for a high number of reproducible experiments in a short time. Nevertheless, the partly open nature of the setup (e.g., during MEA measurements) makes constant temperature control difficult and should be kept to a minimum. This described method makes it possible to analyze and modulate the intracardiac autonomic nervous system in decentralized hearts.",
keywords = "Journal Article, Video-Audio Media, Research Support, Non-U.S. Gov't",
author = "Christiane Jungen and Katharina Scherschel and Bork, {Nadja I} and Pawel Kuklik and Christian Eickholt and Helge Kniep and Niklas Klatt and Stephan Willems and Nikolaev, {Viacheslav O} and Christian Meyer",
year = "2018",
month = may,
day = "22",
doi = "10.3791/57617",
language = "English",
journal = "JOVE-J VIS EXP",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "135",

}

RIS

TY - JOUR

T1 - Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System

AU - Jungen, Christiane

AU - Scherschel, Katharina

AU - Bork, Nadja I

AU - Kuklik, Pawel

AU - Eickholt, Christian

AU - Kniep, Helge

AU - Klatt, Niklas

AU - Willems, Stephan

AU - Nikolaev, Viacheslav O

AU - Meyer, Christian

PY - 2018/5/22

Y1 - 2018/5/22

N2 - Since its invention in the late 19th century, the Langendorff ex vivo heart perfusion system continues to be a relevant tool for studying a broad spectrum of physiological, biochemical, morphological, and pharmacological parameters in centrally denervated hearts. Here, we describe a setup for the modulation of the intracardiac autonomic nervous system and the assessment of its influence on basic electrophysiology, arrhythmogenesis, and cyclic adenosine monophosphate (cAMP) dynamics. The intracardiac autonomic nervous system is modulated by the mechanical dissection of atrial fat pads-in which murine ganglia are located mainly-or by the usage of global as well as targeted pharmacological interventions. An octapolar electrophysiological catheter is introduced into the right atrium and the right ventricle, and epicardial-placed multi-electrode arrays (MEA) for high-resolution mapping are used to determine cardiac electrophysiology and arrhythmogenesis. Förster resonance energy transfer (FRET) imaging is performed for the real-time monitoring of cAMP levels in different cardiac regions. Neuromorphology is studied by means of antibody-based staining of whole hearts using neuronal markers to guide the identification and modulation of specific targets of the intracardiac autonomic nervous system in the performed studies. The ex vivo Langendorff setup allows for a high number of reproducible experiments in a short time. Nevertheless, the partly open nature of the setup (e.g., during MEA measurements) makes constant temperature control difficult and should be kept to a minimum. This described method makes it possible to analyze and modulate the intracardiac autonomic nervous system in decentralized hearts.

AB - Since its invention in the late 19th century, the Langendorff ex vivo heart perfusion system continues to be a relevant tool for studying a broad spectrum of physiological, biochemical, morphological, and pharmacological parameters in centrally denervated hearts. Here, we describe a setup for the modulation of the intracardiac autonomic nervous system and the assessment of its influence on basic electrophysiology, arrhythmogenesis, and cyclic adenosine monophosphate (cAMP) dynamics. The intracardiac autonomic nervous system is modulated by the mechanical dissection of atrial fat pads-in which murine ganglia are located mainly-or by the usage of global as well as targeted pharmacological interventions. An octapolar electrophysiological catheter is introduced into the right atrium and the right ventricle, and epicardial-placed multi-electrode arrays (MEA) for high-resolution mapping are used to determine cardiac electrophysiology and arrhythmogenesis. Förster resonance energy transfer (FRET) imaging is performed for the real-time monitoring of cAMP levels in different cardiac regions. Neuromorphology is studied by means of antibody-based staining of whole hearts using neuronal markers to guide the identification and modulation of specific targets of the intracardiac autonomic nervous system in the performed studies. The ex vivo Langendorff setup allows for a high number of reproducible experiments in a short time. Nevertheless, the partly open nature of the setup (e.g., during MEA measurements) makes constant temperature control difficult and should be kept to a minimum. This described method makes it possible to analyze and modulate the intracardiac autonomic nervous system in decentralized hearts.

KW - Journal Article

KW - Video-Audio Media

KW - Research Support, Non-U.S. Gov't

U2 - 10.3791/57617

DO - 10.3791/57617

M3 - SCORING: Journal article

C2 - 29889210

JO - JOVE-J VIS EXP

JF - JOVE-J VIS EXP

SN - 1940-087X

IS - 135

ER -