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Immunohistologic analysis of zygapophyseal joints in patients with ankylosing spondylitis

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Immunohistologic analysis of zygapophyseal joints in patients with ankylosing spondylitis. / Appel, Heiner; Kuhne, Maren; Spiekermann, Simone; Ebhardt, Harald; Grozdanovic, Zarko; Köhler, Dorothee; Dreimann, Marc; Hempfing, Axel; Rudwaleit, Martin; Stein, Harald; Metz-Stavenhagen, Peter; Sieper, Joachim; Loddenkemper, Christoph.

in: ARTHRITIS RHEUM-US, Jahrgang 54, Nr. 9, 01.09.2006, S. 2845-51.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Appel, H, Kuhne, M, Spiekermann, S, Ebhardt, H, Grozdanovic, Z, Köhler, D, Dreimann, M, Hempfing, A, Rudwaleit, M, Stein, H, Metz-Stavenhagen, P, Sieper, J & Loddenkemper, C 2006, 'Immunohistologic analysis of zygapophyseal joints in patients with ankylosing spondylitis', ARTHRITIS RHEUM-US, Jg. 54, Nr. 9, S. 2845-51. https://doi.org/10.1002/art.22060

APA

Appel, H., Kuhne, M., Spiekermann, S., Ebhardt, H., Grozdanovic, Z., Köhler, D., Dreimann, M., Hempfing, A., Rudwaleit, M., Stein, H., Metz-Stavenhagen, P., Sieper, J., & Loddenkemper, C. (2006). Immunohistologic analysis of zygapophyseal joints in patients with ankylosing spondylitis. ARTHRITIS RHEUM-US, 54(9), 2845-51. https://doi.org/10.1002/art.22060

Vancouver

Appel H, Kuhne M, Spiekermann S, Ebhardt H, Grozdanovic Z, Köhler D et al. Immunohistologic analysis of zygapophyseal joints in patients with ankylosing spondylitis. ARTHRITIS RHEUM-US. 2006 Sep 1;54(9):2845-51. https://doi.org/10.1002/art.22060

Bibtex

@article{1fd2ca1f0440407eb6dad47aba79b4dd,
title = "Immunohistologic analysis of zygapophyseal joints in patients with ankylosing spondylitis",
abstract = "OBJECTIVE: Zygapophyseal joints of the spine are often affected in ankylosing spondylitis (AS). In this study, we undertook a systematic immunohistologic evaluation of the immunopathology of the zygapophyseal joints in patients with advanced AS.METHODS: We obtained zygapophyseal joints from 16 AS patients undergoing polysegmental correction of kyphosis and from 10 non-AS controls (at autopsy). Immunohistologic analysis of the bone marrow was performed by analyzing the number of infiltrating T cells (CD3, CD4, CD8), B cells (CD20), osteoclasts (CD68), bone marrow macrophages (CD68), and microvessel density (CD34) per high-power field.RESULTS: Zygapophyseal joints from 6 of 16 AS patients, but from none of the controls, exhibited 2 or more CD3+ T cell aggregates, signifying persistent inflammation. Interstitial CD4+ and CD8+ T cells were significantly more frequent in AS patients compared with non-AS controls (P = 0.002 and P = 0.049, respectively). While there was no clear difference between the number of CD20+ B cells in AS patients overall compared with controls, there was a significant difference when persistently inflamed joints from patients with AS were compared with joints without active inflammation from patients with AS or joints from controls (both P = 0.03). Microvessel density in bone marrow from AS patients with active inflammation was significantly higher than that in bone marrow from controls.CONCLUSION: This immunohistologic study of bone marrow from zygapophyseal joints demonstrates persistent inflammation in the spine of patients with AS, including those with longstanding disease. The findings of increased numbers of T cells and B cells and neoangiogenesis suggest that these features play a role in the pathogenesis of AS.",
keywords = "Antigens, CD, Antigens, CD3, Arthrography, Autopsy, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Humans, Immunohistochemistry, Reference Values, Spondylitis, Ankylosing, T-Lymphocytes, Zygapophyseal Joint",
author = "Heiner Appel and Maren Kuhne and Simone Spiekermann and Harald Ebhardt and Zarko Grozdanovic and Dorothee K{\"o}hler and Marc Dreimann and Axel Hempfing and Martin Rudwaleit and Harald Stein and Peter Metz-Stavenhagen and Joachim Sieper and Christoph Loddenkemper",
year = "2006",
month = sep,
day = "1",
doi = "10.1002/art.22060",
language = "English",
volume = "54",
pages = "2845--51",
journal = "ARTHRITIS RHEUMATOL",
issn = "2326-5191",
publisher = "John Wiley and Sons Ltd",
number = "9",

}

RIS

TY - JOUR

T1 - Immunohistologic analysis of zygapophyseal joints in patients with ankylosing spondylitis

AU - Appel, Heiner

AU - Kuhne, Maren

AU - Spiekermann, Simone

AU - Ebhardt, Harald

AU - Grozdanovic, Zarko

AU - Köhler, Dorothee

AU - Dreimann, Marc

AU - Hempfing, Axel

AU - Rudwaleit, Martin

AU - Stein, Harald

AU - Metz-Stavenhagen, Peter

AU - Sieper, Joachim

AU - Loddenkemper, Christoph

PY - 2006/9/1

Y1 - 2006/9/1

N2 - OBJECTIVE: Zygapophyseal joints of the spine are often affected in ankylosing spondylitis (AS). In this study, we undertook a systematic immunohistologic evaluation of the immunopathology of the zygapophyseal joints in patients with advanced AS.METHODS: We obtained zygapophyseal joints from 16 AS patients undergoing polysegmental correction of kyphosis and from 10 non-AS controls (at autopsy). Immunohistologic analysis of the bone marrow was performed by analyzing the number of infiltrating T cells (CD3, CD4, CD8), B cells (CD20), osteoclasts (CD68), bone marrow macrophages (CD68), and microvessel density (CD34) per high-power field.RESULTS: Zygapophyseal joints from 6 of 16 AS patients, but from none of the controls, exhibited 2 or more CD3+ T cell aggregates, signifying persistent inflammation. Interstitial CD4+ and CD8+ T cells were significantly more frequent in AS patients compared with non-AS controls (P = 0.002 and P = 0.049, respectively). While there was no clear difference between the number of CD20+ B cells in AS patients overall compared with controls, there was a significant difference when persistently inflamed joints from patients with AS were compared with joints without active inflammation from patients with AS or joints from controls (both P = 0.03). Microvessel density in bone marrow from AS patients with active inflammation was significantly higher than that in bone marrow from controls.CONCLUSION: This immunohistologic study of bone marrow from zygapophyseal joints demonstrates persistent inflammation in the spine of patients with AS, including those with longstanding disease. The findings of increased numbers of T cells and B cells and neoangiogenesis suggest that these features play a role in the pathogenesis of AS.

AB - OBJECTIVE: Zygapophyseal joints of the spine are often affected in ankylosing spondylitis (AS). In this study, we undertook a systematic immunohistologic evaluation of the immunopathology of the zygapophyseal joints in patients with advanced AS.METHODS: We obtained zygapophyseal joints from 16 AS patients undergoing polysegmental correction of kyphosis and from 10 non-AS controls (at autopsy). Immunohistologic analysis of the bone marrow was performed by analyzing the number of infiltrating T cells (CD3, CD4, CD8), B cells (CD20), osteoclasts (CD68), bone marrow macrophages (CD68), and microvessel density (CD34) per high-power field.RESULTS: Zygapophyseal joints from 6 of 16 AS patients, but from none of the controls, exhibited 2 or more CD3+ T cell aggregates, signifying persistent inflammation. Interstitial CD4+ and CD8+ T cells were significantly more frequent in AS patients compared with non-AS controls (P = 0.002 and P = 0.049, respectively). While there was no clear difference between the number of CD20+ B cells in AS patients overall compared with controls, there was a significant difference when persistently inflamed joints from patients with AS were compared with joints without active inflammation from patients with AS or joints from controls (both P = 0.03). Microvessel density in bone marrow from AS patients with active inflammation was significantly higher than that in bone marrow from controls.CONCLUSION: This immunohistologic study of bone marrow from zygapophyseal joints demonstrates persistent inflammation in the spine of patients with AS, including those with longstanding disease. The findings of increased numbers of T cells and B cells and neoangiogenesis suggest that these features play a role in the pathogenesis of AS.

KW - Antigens, CD

KW - Antigens, CD3

KW - Arthrography

KW - Autopsy

KW - CD4-Positive T-Lymphocytes

KW - CD8-Positive T-Lymphocytes

KW - Humans

KW - Immunohistochemistry

KW - Reference Values

KW - Spondylitis, Ankylosing

KW - T-Lymphocytes

KW - Zygapophyseal Joint

U2 - 10.1002/art.22060

DO - 10.1002/art.22060

M3 - SCORING: Journal article

C2 - 16947385

VL - 54

SP - 2845

EP - 2851

JO - ARTHRITIS RHEUMATOL

JF - ARTHRITIS RHEUMATOL

SN - 2326-5191

IS - 9

ER -