Immunohistochemical detection of extracellular matrix proteins in the irradiated rat mandibular gland
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Immunohistochemical detection of extracellular matrix proteins in the irradiated rat mandibular gland. / Bartel-Friedrich, S; Friedrich, R E; Moll, R; Lautenschlager, C.
in: ANTICANCER RES, Jahrgang 19, Nr. 4A, 1999, S. 2779-84.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Immunohistochemical detection of extracellular matrix proteins in the irradiated rat mandibular gland
AU - Bartel-Friedrich, S
AU - Friedrich, R E
AU - Moll, R
AU - Lautenschlager, C
PY - 1999
Y1 - 1999
N2 - UNLABELLED: BACKGROUND/MATERIAL AND METHODS: In 59 rat mandibular glands we investigated the distribution of extracellular matrix proteins (EMP) in rat salivary gland tumors. The animals differed in age and pretreatment status (irradiation versus no irradiation). For immunohistochemical analysis we used polyclonal EMP antibodies [anti-laminin (AL), anti-fibronectin (AF), anti-collagen-III (AC-III), anti-collagen-IV (AC-IV)].RESULTS: The antibodies identified the different structures of the extracellular matrix: components of basement membranes [(BM); laminin, collagen IV] and components of BM-associated structures (fibronectin, collagen III). Immunoreactivity was found in glandular tissue, in vascular walls, in nerve tissue and the interstitial connective tissue. Various EMP showed different patterns. Significant differences were seen between irradiated and non-irradiated glands, often with generally stronger and more extended staining in the irradiated group. Thus, both nerve tissue and excretory ducts (ECD) were in part strongly stained for AL, while ECD were intensely positive for AF.CONCLUSIONS: Preceding radiation has to be considered when interpreting salivary gland tissue, especially in tumor differentiation studies following irradiation.
AB - UNLABELLED: BACKGROUND/MATERIAL AND METHODS: In 59 rat mandibular glands we investigated the distribution of extracellular matrix proteins (EMP) in rat salivary gland tumors. The animals differed in age and pretreatment status (irradiation versus no irradiation). For immunohistochemical analysis we used polyclonal EMP antibodies [anti-laminin (AL), anti-fibronectin (AF), anti-collagen-III (AC-III), anti-collagen-IV (AC-IV)].RESULTS: The antibodies identified the different structures of the extracellular matrix: components of basement membranes [(BM); laminin, collagen IV] and components of BM-associated structures (fibronectin, collagen III). Immunoreactivity was found in glandular tissue, in vascular walls, in nerve tissue and the interstitial connective tissue. Various EMP showed different patterns. Significant differences were seen between irradiated and non-irradiated glands, often with generally stronger and more extended staining in the irradiated group. Thus, both nerve tissue and excretory ducts (ECD) were in part strongly stained for AL, while ECD were intensely positive for AF.CONCLUSIONS: Preceding radiation has to be considered when interpreting salivary gland tissue, especially in tumor differentiation studies following irradiation.
KW - Animals
KW - Antibodies
KW - Collagen
KW - Extracellular Matrix Proteins
KW - Female
KW - Fibronectins
KW - Laminin
KW - Neoplasms, Radiation-Induced
KW - Rats
KW - Rats, Wistar
KW - Submandibular Gland
KW - Submandibular Gland Neoplasms
KW - Time Factors
KW - Tumor Markers, Biological
M3 - SCORING: Journal article
C2 - 10470241
VL - 19
SP - 2779
EP - 2784
JO - ANTICANCER RES
JF - ANTICANCER RES
SN - 0250-7005
IS - 4A
ER -