Immunhistochemischer Nachweis von Hormonen in Hypophysenadenomen der Ratte nach fraktionierter Bestrahlung
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Immunhistochemischer Nachweis von Hormonen in Hypophysenadenomen der Ratte nach fraktionierter Bestrahlung. / Bartel-Friedrich, S; Saeger, W; Friedrich, R E; Laas, R.
in: LARYNGO RHINO OTOL, Jahrgang 80, Nr. 5, 01.05.2001, S. 257-62.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Immunhistochemischer Nachweis von Hormonen in Hypophysenadenomen der Ratte nach fraktionierter Bestrahlung
AU - Bartel-Friedrich, S
AU - Saeger, W
AU - Friedrich, R E
AU - Laas, R
PY - 2001/5/1
Y1 - 2001/5/1
N2 - BACKGROUND: The aim of this study was to determine the hormones produced in pituitary gland tumours (TM) following fractionated external irradiation in rats. The TM arose in the course of studies on other questions of radiation effects.METHODS: The left neck and skull base of sixty female Wistar rats, 3 to 4 months of age at the beginning of the external irradiation, were subjected to roentgen rays exposure, fractionated to 2 Grays daily, either up to 20, 40 or 60 Gy. The midline of the neck and skull was always inside the radiation field. The animals were sacrificed either 6 months or 12 months after completion of the randomly assigned irradiation protocol. Five non-irradiated rats served as controls.RESULTS: No TM developed in the non-irradiated animals. Out of 60 irradiated rats 9 developed a pituitary TM (15%). In remnants of the normal adenohypophysis we revealed immunohistochemical reactivity for growth hormone (GH), adrenocorticotropic hormone (ACTH), prolactin (PRL), thyroid stimulating hormone (TSH), and follicle stimulating hormone (FSH). In the radiation-induced adenomas, 4 TM were immuno-reactive for TSH, 4 for GH, 2 for PRL, and 2 for FSH. The TM did not react with anti-ACTH and anti-LH (luteinizing hormone) anti-sera.CONCLUSIONS: Obviously, irradiation accelerates the development of pituitary gland adenomas. In this series the TM were predominantly incidental findings at necropsies after pretermed follow-up intervals with a variety of hormone-producing cells. On the other hand, four of nine TM were null adenomas. Necropsies of the brain should be carried out routinely in irradiation studies of the skull.
AB - BACKGROUND: The aim of this study was to determine the hormones produced in pituitary gland tumours (TM) following fractionated external irradiation in rats. The TM arose in the course of studies on other questions of radiation effects.METHODS: The left neck and skull base of sixty female Wistar rats, 3 to 4 months of age at the beginning of the external irradiation, were subjected to roentgen rays exposure, fractionated to 2 Grays daily, either up to 20, 40 or 60 Gy. The midline of the neck and skull was always inside the radiation field. The animals were sacrificed either 6 months or 12 months after completion of the randomly assigned irradiation protocol. Five non-irradiated rats served as controls.RESULTS: No TM developed in the non-irradiated animals. Out of 60 irradiated rats 9 developed a pituitary TM (15%). In remnants of the normal adenohypophysis we revealed immunohistochemical reactivity for growth hormone (GH), adrenocorticotropic hormone (ACTH), prolactin (PRL), thyroid stimulating hormone (TSH), and follicle stimulating hormone (FSH). In the radiation-induced adenomas, 4 TM were immuno-reactive for TSH, 4 for GH, 2 for PRL, and 2 for FSH. The TM did not react with anti-ACTH and anti-LH (luteinizing hormone) anti-sera.CONCLUSIONS: Obviously, irradiation accelerates the development of pituitary gland adenomas. In this series the TM were predominantly incidental findings at necropsies after pretermed follow-up intervals with a variety of hormone-producing cells. On the other hand, four of nine TM were null adenomas. Necropsies of the brain should be carried out routinely in irradiation studies of the skull.
KW - Adenoma
KW - Animals
KW - Dose Fractionation
KW - Female
KW - Neoplasms, Radiation-Induced
KW - Paraneoplastic Endocrine Syndromes
KW - Pituitary Gland
KW - Pituitary Hormones
KW - Pituitary Neoplasms
KW - Rats
KW - Rats, Wistar
U2 - 10.1055/s-2001-13887
DO - 10.1055/s-2001-13887
M3 - SCORING: Zeitschriftenaufsatz
C2 - 11417248
VL - 80
SP - 257
EP - 262
JO - LARYNGO RHINO OTOL
JF - LARYNGO RHINO OTOL
SN - 0935-8943
IS - 5
ER -