Immune cell infiltration in malignant middle cerebral artery infarction:comparison with transient cerebral ischemia
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Immune cell infiltration in malignant middle cerebral artery infarction:comparison with transient cerebral ischemia. / Chu, Hannah X; Kim, Hyun Ah; Lee, Seyoung; Moore, Jeffrey P; Chan, Christopher T; Vinh, Antony; Gelderblom, Mathias; Arumugam, Thiruma V; Broughton, Brad R S; Drummond, Grant R; Sobey, Christopher G.
in: J CEREBR BLOOD F MET, Jahrgang 34, Nr. 3, 2014, S. 450-459.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Immune cell infiltration in malignant middle cerebral artery infarction:comparison with transient cerebral ischemia
AU - Chu, Hannah X
AU - Kim, Hyun Ah
AU - Lee, Seyoung
AU - Moore, Jeffrey P
AU - Chan, Christopher T
AU - Vinh, Antony
AU - Gelderblom, Mathias
AU - Arumugam, Thiruma V
AU - Broughton, Brad R S
AU - Drummond, Grant R
AU - Sobey, Christopher G
PY - 2014
Y1 - 2014
N2 - We tested whether significant leukocyte infiltration occurs in a mouse model of permanent cerebral ischemia. C57BL6/J male mice underwent either permanent (3 or 24 hours) or transient (1 or 2 hours+22- to 23-hour reperfusion) middle cerebral artery occlusion (MCAO). Using flow cytometry, we observed ∼15,000 leukocytes (CD45(+high) cells) in the ischemic hemisphere as early as 3 hours after permanent MCAO (pMCAO), comprising ∼40% lymphoid cells and ∼60% myeloid cells. Neutrophils were the predominant cell type entering the brain, and were increased to ∼5,000 as early as 3 hours after pMCAO. Several cell types (monocytes, macrophages, B lymphocytes, CD8(+) T lymphocytes, and natural killer cells) were also increased at 3 hours to levels sustained for 24 hours, whereas others (CD4(+) T cells, natural killer T cells, and dendritic cells) were unchanged at 3 hours, but were increased by 24 hours after pMCAO. Immunohistochemical analysis revealed that leukocytes typically had entered and widely dispersed throughout the parenchyma of the infarct within 3 hours. Moreover, compared with pMCAO, there were ∼50% fewer infiltrating leukocytes at 24 hours after transient MCAO (tMCAO), independent of infarct size. Microglial cell numbers were bilaterally increased in both models. These findings indicate that a profound infiltration of inflammatory cells occurs in the brain early after focal ischemia, especially without reperfusion.
AB - We tested whether significant leukocyte infiltration occurs in a mouse model of permanent cerebral ischemia. C57BL6/J male mice underwent either permanent (3 or 24 hours) or transient (1 or 2 hours+22- to 23-hour reperfusion) middle cerebral artery occlusion (MCAO). Using flow cytometry, we observed ∼15,000 leukocytes (CD45(+high) cells) in the ischemic hemisphere as early as 3 hours after permanent MCAO (pMCAO), comprising ∼40% lymphoid cells and ∼60% myeloid cells. Neutrophils were the predominant cell type entering the brain, and were increased to ∼5,000 as early as 3 hours after pMCAO. Several cell types (monocytes, macrophages, B lymphocytes, CD8(+) T lymphocytes, and natural killer cells) were also increased at 3 hours to levels sustained for 24 hours, whereas others (CD4(+) T cells, natural killer T cells, and dendritic cells) were unchanged at 3 hours, but were increased by 24 hours after pMCAO. Immunohistochemical analysis revealed that leukocytes typically had entered and widely dispersed throughout the parenchyma of the infarct within 3 hours. Moreover, compared with pMCAO, there were ∼50% fewer infiltrating leukocytes at 24 hours after transient MCAO (tMCAO), independent of infarct size. Microglial cell numbers were bilaterally increased in both models. These findings indicate that a profound infiltration of inflammatory cells occurs in the brain early after focal ischemia, especially without reperfusion.
KW - Animals
KW - Chemotaxis, Leukocyte
KW - Disease Models, Animal
KW - Flow Cytometry
KW - Immunohistochemistry
KW - Infarction, Middle Cerebral Artery
KW - Ischemic Attack, Transient
KW - Leukocyte Count
KW - Lymphocyte Count
KW - Lymphocytes
KW - Male
KW - Mice
KW - Mice, Inbred C57BL
KW - Neutrophil Infiltration
KW - Time Factors
U2 - 10.1038/jcbfm.2013.217
DO - 10.1038/jcbfm.2013.217
M3 - SCORING: Journal article
C2 - 24326388
VL - 34
SP - 450
EP - 459
JO - J CEREBR BLOOD F MET
JF - J CEREBR BLOOD F MET
SN - 0271-678X
IS - 3
ER -