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Imaging Synaptic Glutamate Release with Two-Photon Microscopy in Organotypic Slice Cultures

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Imaging Synaptic Glutamate Release with Two-Photon Microscopy in Organotypic Slice Cultures. / Dürst, Céline D.; Oertner, Thomas G.

Synaptic Vesicles: Methods and Protocols. Hrsg. / Jana Dahlmanns; Marc Dahlmanns. Band 2417 1. Aufl. New York, NY : HUMANA PRESS INC, 2022. S. 205-219 (Methods in molecular biology (Clifton, N.J.)).

Publikationen: SCORING: Beitrag in Buch/Sammelwerk › SCORING: Beitrag in Sammelwerk › Forschung › Begutachtung

Harvard

Dürst, CD & Oertner, TG 2022, Imaging Synaptic Glutamate Release with Two-Photon Microscopy in Organotypic Slice Cultures. in J Dahlmanns & M Dahlmanns (Hrsg.), Synaptic Vesicles: Methods and Protocols. 1 Aufl., Bd. 2417, Methods in molecular biology (Clifton, N.J.), HUMANA PRESS INC, New York, NY, S. 205-219. https://doi.org/10.1007/978-1-0716-1916-2_16

APA

Dürst, C. D., & Oertner, T. G. (2022). Imaging Synaptic Glutamate Release with Two-Photon Microscopy in Organotypic Slice Cultures. in J. Dahlmanns, & M. Dahlmanns (Hrsg.), Synaptic Vesicles: Methods and Protocols (1 Aufl., Band 2417, S. 205-219). (Methods in molecular biology (Clifton, N.J.)). HUMANA PRESS INC. https://doi.org/10.1007/978-1-0716-1916-2_16

Vancouver

Dürst CD, Oertner TG. Imaging Synaptic Glutamate Release with Two-Photon Microscopy in Organotypic Slice Cultures. in Dahlmanns J, Dahlmanns M, Hrsg., Synaptic Vesicles: Methods and Protocols. 1 Aufl. Band 2417. New York, NY: HUMANA PRESS INC. 2022. S. 205-219. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-1916-2_16

Bibtex

@inbook{e887f1be20dc4c678750c13442eacb3d,
title = "Imaging Synaptic Glutamate Release with Two-Photon Microscopy in Organotypic Slice Cultures",
abstract = "The strength of an excitatory synapse relies on the amount of glutamate it releases and on the amount of postsynaptic receptors responding to the released glutamate. Here we describe a strategy to investigate presynaptic release independently of postsynaptic receptors, using a genetically encoded glutamate indicator (GEGI) such as iGluSnFR to measure synaptic transmission in rodent organotypic slice cultures. We express the iGluSnFR in CA3 pyramidal cells and perform two-photon glutamate imaging on individual Schaffer collateral boutons in CA1. Sparse labeling is achieved via transfection of pyramidal cells in organotypic hippocampal cultures, and imaging of evoked glutamate transients with two-photon laser scanning microscopy. A spiral scan path over an individual presynaptic bouton allows to sample at high temporal resolution the local release site in order to capture the peak of iGluSnFR transients.",
keywords = "Genetically encoded glutamate indicators (GEGIs), Glutamate, iGluSnFR, Organotypic slice cultures, Synaptic transmission, Two-photon imaging",
author = "D{\"u}rst, {C{\'e}line D.} and Oertner, {Thomas G.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1916-2_16",
language = "English",
isbn = "978-1-0716-1915-5",
volume = "2417",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "HUMANA PRESS INC",
pages = "205--219",
editor = "Jana Dahlmanns and Marc Dahlmanns",
booktitle = "Synaptic Vesicles",
edition = "1",

}

RIS

TY - CHAP

T1 - Imaging Synaptic Glutamate Release with Two-Photon Microscopy in Organotypic Slice Cultures

AU - Dürst, Céline D.

AU - Oertner, Thomas G.

N1 - Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - The strength of an excitatory synapse relies on the amount of glutamate it releases and on the amount of postsynaptic receptors responding to the released glutamate. Here we describe a strategy to investigate presynaptic release independently of postsynaptic receptors, using a genetically encoded glutamate indicator (GEGI) such as iGluSnFR to measure synaptic transmission in rodent organotypic slice cultures. We express the iGluSnFR in CA3 pyramidal cells and perform two-photon glutamate imaging on individual Schaffer collateral boutons in CA1. Sparse labeling is achieved via transfection of pyramidal cells in organotypic hippocampal cultures, and imaging of evoked glutamate transients with two-photon laser scanning microscopy. A spiral scan path over an individual presynaptic bouton allows to sample at high temporal resolution the local release site in order to capture the peak of iGluSnFR transients.

AB - The strength of an excitatory synapse relies on the amount of glutamate it releases and on the amount of postsynaptic receptors responding to the released glutamate. Here we describe a strategy to investigate presynaptic release independently of postsynaptic receptors, using a genetically encoded glutamate indicator (GEGI) such as iGluSnFR to measure synaptic transmission in rodent organotypic slice cultures. We express the iGluSnFR in CA3 pyramidal cells and perform two-photon glutamate imaging on individual Schaffer collateral boutons in CA1. Sparse labeling is achieved via transfection of pyramidal cells in organotypic hippocampal cultures, and imaging of evoked glutamate transients with two-photon laser scanning microscopy. A spiral scan path over an individual presynaptic bouton allows to sample at high temporal resolution the local release site in order to capture the peak of iGluSnFR transients.

KW - Genetically encoded glutamate indicators (GEGIs)

KW - Glutamate

KW - iGluSnFR

KW - Organotypic slice cultures

KW - Synaptic transmission

KW - Two-photon imaging

UR - http://www.scopus.com/inward/record.url?scp=85123905342&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-1916-2_16

DO - 10.1007/978-1-0716-1916-2_16

M3 - SCORING: Contribution to collected editions/anthologies

C2 - 35099802

AN - SCOPUS:85123905342

SN - 978-1-0716-1915-5

VL - 2417

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 205

EP - 219

BT - Synaptic Vesicles

A2 - Dahlmanns, Jana

A2 - Dahlmanns, Marc

PB - HUMANA PRESS INC

CY - New York, NY

ER -