Imaging of the murine biliopancreatic tract at 7 tesla: Technique and results in a model of primary sclerosing cholangitis

TY - JOUR

T1 - Imaging of the murine biliopancreatic tract at 7 tesla: Technique and results in a model of primary sclerosing cholangitis

AU - Ernst, Thomas M

AU - Schwinge, Dorothee

AU - Raabe, Nina

AU - Daubmann, Anne

AU - Kaul, Michael G

AU - Adam, Gerhard

AU - Schramm, Christoph

AU - Ittrich, Harald

N1 - Copyright © 2013 Wiley Periodicals, Inc.

PY - 2014/12

Y1 - 2014/12

N2 - PURPOSE: To assess the feasibility of a 7 Tesla (T) MR cholangiopancreatography (MRCP) protocol to image the morphology and detect and intraindividually monitor pathological changes of the biliopancreatic tract in a mouse model of primary sclerosing cholangitis (PSC).MATERIALS AND METHODS: Six female Mdr2(Abcb)(-/-) mice, a well-established model of PSC, were imaged five times during weeks 10-19. Three wild-type controls were imaged at age 15 weeks. MRCP acquisition with three-dimensional fast recovery fast spin echo sequences (3D-FRFSE) was performed using three sequences with different resolutions, repetition times (TR), and with/without respiration-gating in a 7T preclinical MRI system. Image quality and visualization of five biliopancreatic structures were evaluated by three independent readers.RESULTS: Image quality was rated diagnostically sufficient in 86% of the datasets acquired without gating and in 100% for the respiration-gated sequences. Intrahepatic ducts were well visualized (≥97%) in Mdr2(-/-) mice. Stenoses and dilatations of the biliary ducts were intraindividually monitored. Progression and regression of bile duct pathologies were sufficiently assessed during the observation time.CONCLUSION: High-quality respiration-gated MRCP of the Mdr2(-/-) PSC model at 7T allows for in vivo imaging of murine biliopancreatic tract and monitoring of bile duct pathologies, permitting longitudinal intraindividual studies in murine models of inflammatory bile duct diseases. J. Magn. Reson. Imaging 2013;. © 2013 Wiley Periodicals, Inc.

AB - PURPOSE: To assess the feasibility of a 7 Tesla (T) MR cholangiopancreatography (MRCP) protocol to image the morphology and detect and intraindividually monitor pathological changes of the biliopancreatic tract in a mouse model of primary sclerosing cholangitis (PSC).MATERIALS AND METHODS: Six female Mdr2(Abcb)(-/-) mice, a well-established model of PSC, were imaged five times during weeks 10-19. Three wild-type controls were imaged at age 15 weeks. MRCP acquisition with three-dimensional fast recovery fast spin echo sequences (3D-FRFSE) was performed using three sequences with different resolutions, repetition times (TR), and with/without respiration-gating in a 7T preclinical MRI system. Image quality and visualization of five biliopancreatic structures were evaluated by three independent readers.RESULTS: Image quality was rated diagnostically sufficient in 86% of the datasets acquired without gating and in 100% for the respiration-gated sequences. Intrahepatic ducts were well visualized (≥97%) in Mdr2(-/-) mice. Stenoses and dilatations of the biliary ducts were intraindividually monitored. Progression and regression of bile duct pathologies were sufficiently assessed during the observation time.CONCLUSION: High-quality respiration-gated MRCP of the Mdr2(-/-) PSC model at 7T allows for in vivo imaging of murine biliopancreatic tract and monitoring of bile duct pathologies, permitting longitudinal intraindividual studies in murine models of inflammatory bile duct diseases. J. Magn. Reson. Imaging 2013;. © 2013 Wiley Periodicals, Inc.

U2 - 10.1002/jmri.24475

DO - 10.1002/jmri.24475

M3 - SCORING: Journal article

C2 - 24227696

VL - 40

SP - 1355

EP - 1364

JO - J MAGN RESON IMAGING

JF - J MAGN RESON IMAGING

SN - 1053-1807

IS - 6

ER -