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Imaging of molecular surface dynamics in brain slices using single-particle tracking

Standard

Imaging of molecular surface dynamics in brain slices using single-particle tracking. / Biermann, B; Sokoll, S; Klueva, J; Missler, M; Wiegert, Simon; Sibarita, J-B; Heine, M.

in: NAT COMMUN, Jahrgang 5, 01.01.2014, S. 3024.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Biermann, B, Sokoll, S, Klueva, J, Missler, M, Wiegert, S, Sibarita, J-B & Heine, M 2014, 'Imaging of molecular surface dynamics in brain slices using single-particle tracking', NAT COMMUN, Jg. 5, S. 3024. https://doi.org/10.1038/ncomms4024

APA

Biermann, B., Sokoll, S., Klueva, J., Missler, M., Wiegert, S., Sibarita, J-B., & Heine, M. (2014). Imaging of molecular surface dynamics in brain slices using single-particle tracking. NAT COMMUN, 5, 3024. https://doi.org/10.1038/ncomms4024

Vancouver

Biermann B, Sokoll S, Klueva J, Missler M, Wiegert S, Sibarita J-B et al. Imaging of molecular surface dynamics in brain slices using single-particle tracking. NAT COMMUN. 2014 Jan 1;5:3024. https://doi.org/10.1038/ncomms4024

Bibtex

@article{88a99516565c4f14b17d937b8b18b51a,
title = "Imaging of molecular surface dynamics in brain slices using single-particle tracking",
abstract = "Organization of signalling molecules in biological membranes is crucial for cellular communication. Many receptors, ion channels and cell adhesion molecules are associated with proteins important for their trafficking, surface localization or function. These complexes are embedded in a lipid environment of varying composition. Binding affinities and stoichiometry of such complexes were so far experimentally accessible only in isolated systems or monolayers of cell culture. Visualization of molecular dynamics within signalling complexes and their correlation to specialized membrane compartments demand high temporal and spatial resolution and has been difficult to demonstrate in complex tissue like brain slices. Here we demonstrate the feasibility of single-particle tracking (SPT) in organotypic brain slices to measure molecular dynamics of lipids and transmembrane proteins in correlation to synaptic membrane compartments. This method will provide important information about the dynamics and organization of surface molecules in the complex environment of neuronal networks within brain slices.",
author = "B Biermann and S Sokoll and J Klueva and M Missler and Simon Wiegert and J-B Sibarita and M Heine",
year = "2014",
month = jan,
day = "1",
doi = "10.1038/ncomms4024",
language = "English",
volume = "5",
pages = "3024",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",

}

RIS

TY - JOUR

T1 - Imaging of molecular surface dynamics in brain slices using single-particle tracking

AU - Biermann, B

AU - Sokoll, S

AU - Klueva, J

AU - Missler, M

AU - Wiegert, Simon

AU - Sibarita, J-B

AU - Heine, M

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Organization of signalling molecules in biological membranes is crucial for cellular communication. Many receptors, ion channels and cell adhesion molecules are associated with proteins important for their trafficking, surface localization or function. These complexes are embedded in a lipid environment of varying composition. Binding affinities and stoichiometry of such complexes were so far experimentally accessible only in isolated systems or monolayers of cell culture. Visualization of molecular dynamics within signalling complexes and their correlation to specialized membrane compartments demand high temporal and spatial resolution and has been difficult to demonstrate in complex tissue like brain slices. Here we demonstrate the feasibility of single-particle tracking (SPT) in organotypic brain slices to measure molecular dynamics of lipids and transmembrane proteins in correlation to synaptic membrane compartments. This method will provide important information about the dynamics and organization of surface molecules in the complex environment of neuronal networks within brain slices.

AB - Organization of signalling molecules in biological membranes is crucial for cellular communication. Many receptors, ion channels and cell adhesion molecules are associated with proteins important for their trafficking, surface localization or function. These complexes are embedded in a lipid environment of varying composition. Binding affinities and stoichiometry of such complexes were so far experimentally accessible only in isolated systems or monolayers of cell culture. Visualization of molecular dynamics within signalling complexes and their correlation to specialized membrane compartments demand high temporal and spatial resolution and has been difficult to demonstrate in complex tissue like brain slices. Here we demonstrate the feasibility of single-particle tracking (SPT) in organotypic brain slices to measure molecular dynamics of lipids and transmembrane proteins in correlation to synaptic membrane compartments. This method will provide important information about the dynamics and organization of surface molecules in the complex environment of neuronal networks within brain slices.

U2 - 10.1038/ncomms4024

DO - 10.1038/ncomms4024

M3 - SCORING: Journal article

C2 - 24429796

VL - 5

SP - 3024

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

ER -