If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer.
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If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer. / Bonapace, Laura; Wyckoff, Jeffrey; Oertner, Thomas G.; Jacco, Van Rheenen; Junt, Tobias; Bentires-Alj, Mohamed.
in: J MAMMARY GLAND BIOL, Jahrgang 17, Nr. 2, 2, 01.06.2012, S. 125-129.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer.
AU - Bonapace, Laura
AU - Wyckoff, Jeffrey
AU - Oertner, Thomas G.
AU - Jacco, Van Rheenen
AU - Junt, Tobias
AU - Bentires-Alj, Mohamed
PY - 2012/6/1
Y1 - 2012/6/1
N2 - A fundamental hallmark of cancer is progression to metastasis and the growth of breast cancer metastases in lung, bone, liver and/or brain causes fatal complications. Unfortunately, the cellular and biochemical mechanisms of the metastatic process remain ill-defined. Recent application of intravital multiphoton microscopy (MP-IVM) to image fluorescently labeled cells in mouse models of cancer has allowed dynamic observation of this multi-step process at the cellular and subcellular levels. In this article, we discuss the use of MP-IVM in studies of breast cancer metastasis, as well as surgical techniques for exposing tumors prior to imaging. We also describe a versatile multiphoton microscope for imaging tumor-stroma interactions.
AB - A fundamental hallmark of cancer is progression to metastasis and the growth of breast cancer metastases in lung, bone, liver and/or brain causes fatal complications. Unfortunately, the cellular and biochemical mechanisms of the metastatic process remain ill-defined. Recent application of intravital multiphoton microscopy (MP-IVM) to image fluorescently labeled cells in mouse models of cancer has allowed dynamic observation of this multi-step process at the cellular and subcellular levels. In this article, we discuss the use of MP-IVM in studies of breast cancer metastasis, as well as surgical techniques for exposing tumors prior to imaging. We also describe a versatile multiphoton microscope for imaging tumor-stroma interactions.
KW - Animals
KW - Female
KW - Mice
KW - Cell Line, Tumor
KW - Mice, Transgenic
KW - Immunocompromised Host
KW - Breast Neoplasms/metabolism/pathology
KW - Neoplasm Proteins/genetics/metabolism
KW - Fluorescence Resonance Energy Transfer
KW - Bone Marrow Neoplasms/pathology/secondary
KW - Green Fluorescent Proteins/genetics/metabolism
KW - Lymphatic Metastasis/pathology
KW - Mammary Glands, Animal/metabolism/pathology
KW - Microscopy, Fluorescence, Multiphoton/instrumentation
KW - Neoplasm Transplantation/pathology
KW - Stromal Cells/metabolism/pathology
KW - Animals
KW - Female
KW - Mice
KW - Cell Line, Tumor
KW - Mice, Transgenic
KW - Immunocompromised Host
KW - Breast Neoplasms/metabolism/pathology
KW - Neoplasm Proteins/genetics/metabolism
KW - Fluorescence Resonance Energy Transfer
KW - Bone Marrow Neoplasms/pathology/secondary
KW - Green Fluorescent Proteins/genetics/metabolism
KW - Lymphatic Metastasis/pathology
KW - Mammary Glands, Animal/metabolism/pathology
KW - Microscopy, Fluorescence, Multiphoton/instrumentation
KW - Neoplasm Transplantation/pathology
KW - Stromal Cells/metabolism/pathology
U2 - 10.1007/s10911-012-9250-8
DO - 10.1007/s10911-012-9250-8
M3 - SCORING: Journal article
C2 - 22581273
VL - 17
SP - 125
EP - 129
JO - J MAMMARY GLAND BIOL
JF - J MAMMARY GLAND BIOL
SN - 1083-3021
IS - 2
M1 - 2
ER -
