Identification of the Rage-dependent gene regulatory network in a mouse model of skin inflammation
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Identification of the Rage-dependent gene regulatory network in a mouse model of skin inflammation. / Riehl, Astrid; Bauer, Tobias; Brors, Benedikt; Busch, Hauke; Mark, Regina; Németh, Julia; Gebhardt, Christoffer; Bierhaus, Angelika; Nawroth, Peter; Eils, Roland; König, Rainer; Angel, Peter; Hess, Jochen.
in: BMC GENOMICS, Jahrgang 11, 05.10.2010, S. 537.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Identification of the Rage-dependent gene regulatory network in a mouse model of skin inflammation
AU - Riehl, Astrid
AU - Bauer, Tobias
AU - Brors, Benedikt
AU - Busch, Hauke
AU - Mark, Regina
AU - Németh, Julia
AU - Gebhardt, Christoffer
AU - Bierhaus, Angelika
AU - Nawroth, Peter
AU - Eils, Roland
AU - König, Rainer
AU - Angel, Peter
AU - Hess, Jochen
PY - 2010/10/5
Y1 - 2010/10/5
N2 - BACKGROUND: In the past, molecular mechanisms that drive the initiation of an inflammatory response have been studied intensively. However, corresponding mechanisms that sustain the expression of inflammatory response genes and hence contribute to the establishment of chronic disorders remain poorly understood. Recently, we provided genetic evidence that signaling via the receptor for advanced glycation end products (Rage) drives the strength and maintenance of an inflammatory reaction. In order to decipher the mode of Rage function on gene transcription levels during inflammation, we applied global gene expression profiling on time-resolved samples of mouse back skin, which had been treated with the phorbol ester TPA, a potent inducer of skin inflammation.RESULTS: Ranking of TPA-regulated genes according to their time average mean and peak expression and superimposition of data sets from wild-type (wt) and Rage-deficient mice revealed that Rage signaling is not essential for initial changes in TPA-induced transcription, but absolutely required for sustained alterations in transcript levels. Next, we used a data set of differentially expressed genes between TPA-treated wt and Rage-deficient skin and performed computational analysis of their proximal promoter regions. We found a highly significant enrichment for several transcription factor binding sites (TFBS) leading to the prediction that corresponding transcription factors, such as Sp1, Tcfap2, E2f, Myc and Egr, are regulated by Rage signaling. Accordingly, we could confirm aberrant expression and regulation of members of the E2f protein family in epidermal keratinocytes of Rage-deficient mice.CONCLUSIONS: In summary, our data support the model that engagement of Rage converts a transient cellular stimulation into sustained cellular dysfunction and highlight a novel role of the Rb-E2f pathway in Rage-dependent inflammation during pathological conditions.
AB - BACKGROUND: In the past, molecular mechanisms that drive the initiation of an inflammatory response have been studied intensively. However, corresponding mechanisms that sustain the expression of inflammatory response genes and hence contribute to the establishment of chronic disorders remain poorly understood. Recently, we provided genetic evidence that signaling via the receptor for advanced glycation end products (Rage) drives the strength and maintenance of an inflammatory reaction. In order to decipher the mode of Rage function on gene transcription levels during inflammation, we applied global gene expression profiling on time-resolved samples of mouse back skin, which had been treated with the phorbol ester TPA, a potent inducer of skin inflammation.RESULTS: Ranking of TPA-regulated genes according to their time average mean and peak expression and superimposition of data sets from wild-type (wt) and Rage-deficient mice revealed that Rage signaling is not essential for initial changes in TPA-induced transcription, but absolutely required for sustained alterations in transcript levels. Next, we used a data set of differentially expressed genes between TPA-treated wt and Rage-deficient skin and performed computational analysis of their proximal promoter regions. We found a highly significant enrichment for several transcription factor binding sites (TFBS) leading to the prediction that corresponding transcription factors, such as Sp1, Tcfap2, E2f, Myc and Egr, are regulated by Rage signaling. Accordingly, we could confirm aberrant expression and regulation of members of the E2f protein family in epidermal keratinocytes of Rage-deficient mice.CONCLUSIONS: In summary, our data support the model that engagement of Rage converts a transient cellular stimulation into sustained cellular dysfunction and highlight a novel role of the Rb-E2f pathway in Rage-dependent inflammation during pathological conditions.
KW - Animals
KW - Cluster Analysis
KW - Computational Biology
KW - Disease Models, Animal
KW - E2F Transcription Factors
KW - Gene Expression Profiling
KW - Gene Expression Regulation
KW - Gene Regulatory Networks
KW - Inflammation
KW - Mice
KW - Models, Biological
KW - Promoter Regions, Genetic
KW - Receptor for Advanced Glycation End Products
KW - Receptors, Immunologic
KW - Retinoblastoma Protein
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Signal Transduction
KW - Skin
KW - Tetradecanoylphorbol Acetate
KW - Time Factors
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1186/1471-2164-11-537
DO - 10.1186/1471-2164-11-537
M3 - SCORING: Journal article
C2 - 20923549
VL - 11
SP - 537
JO - BMC GENOMICS
JF - BMC GENOMICS
SN - 1471-2164
ER -
