Identification of reference genes and validation for gene expression studies in diverse axolotl (Ambystoma mexicanum) tissues
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Identification of reference genes and validation for gene expression studies in diverse axolotl (Ambystoma mexicanum) tissues. / Gülke, Eileen; Bucan, Vesna; Liebsch, Christina; Lazaridis, Andrea; Vogt, Peter Maria; Reimers, Kerstin.
in: GENE, Jahrgang 560, Nr. 1, 10.04.2015, S. 114-123.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Identification of reference genes and validation for gene expression studies in diverse axolotl (Ambystoma mexicanum) tissues
AU - Gülke, Eileen
AU - Bucan, Vesna
AU - Liebsch, Christina
AU - Lazaridis, Andrea
AU - Vogt, Peter Maria
AU - Reimers, Kerstin
N1 - Copyright © 2015 Elsevier B.V. All rights reserved.
PY - 2015/4/10
Y1 - 2015/4/10
N2 - For the precise quantitative RT-PCR normalization a set of valid reference genes is obligatory. Moreover have to be taken into concern the experimental conditions as they bias the regulation of reference genes. Up till now, no reference targets have been described for the axolotl (Ambystoma mexicanum). In a search in the public database SalSite for genetic information of the axolotl we identified fourteen presumptive reference genes, eleven of which were further tested for their gene expression stability. This study characterizes the expressional patterns of 11 putative endogenous control genes during axolotl limb regeneration and in an axolotl tissue panel. All 11 reference genes showed variable expression. Strikingly, ACTB was to be found most stable expressed in all comparative tissue groups, so we reason it to be suitable for all different kinds of axolotl tissue-type investigations. Moreover do we suggest GAPDH and RPLP0 as suitable for certain axolotl tissue analysis. When it comes to axolotl limb regeneration, a validated pair of reference genes is ODC and RPLP0. With these findings, new insights into axolotl gene expression profiling might be gained.
AB - For the precise quantitative RT-PCR normalization a set of valid reference genes is obligatory. Moreover have to be taken into concern the experimental conditions as they bias the regulation of reference genes. Up till now, no reference targets have been described for the axolotl (Ambystoma mexicanum). In a search in the public database SalSite for genetic information of the axolotl we identified fourteen presumptive reference genes, eleven of which were further tested for their gene expression stability. This study characterizes the expressional patterns of 11 putative endogenous control genes during axolotl limb regeneration and in an axolotl tissue panel. All 11 reference genes showed variable expression. Strikingly, ACTB was to be found most stable expressed in all comparative tissue groups, so we reason it to be suitable for all different kinds of axolotl tissue-type investigations. Moreover do we suggest GAPDH and RPLP0 as suitable for certain axolotl tissue analysis. When it comes to axolotl limb regeneration, a validated pair of reference genes is ODC and RPLP0. With these findings, new insights into axolotl gene expression profiling might be gained.
KW - Ambystoma mexicanum
KW - Animals
KW - Extremities
KW - Gene Expression Profiling
KW - Genes, Essential
KW - Glyceraldehyde-3-Phosphate Dehydrogenases
KW - Ornithine Decarboxylase
KW - RNA Stability
KW - Reference Standards
KW - Regeneration
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Ribosomal Proteins
KW - Validation Studies as Topic
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.gene.2015.01.030
DO - 10.1016/j.gene.2015.01.030
M3 - SCORING: Journal article
C2 - 25637570
VL - 560
SP - 114
EP - 123
JO - GENE
JF - GENE
SN - 0378-1119
IS - 1
ER -
