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Identification of germ cells at risk for neoplastic transformation in gonadoblastoma

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Identification of germ cells at risk for neoplastic transformation in gonadoblastoma : an immunohistochemical study for OCT3/4 and TSPY. / Kersemaekers, Anne-Marie F; Honecker, Friedemann; Stoop, Hans; Cools, Martine; Molier, Michel; Wolffenbuttel, Katja; Bokemeyer, Carsten; Li, Yunmin; Lau, Yun-Fai Chris; Oosterhuis, J Wolter; Looijenga, Leendert H J.

in: HUM PATHOL, Jahrgang 36, Nr. 5, 05.2005, S. 512-21.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Kersemaekers, A-MF, Honecker, F, Stoop, H, Cools, M, Molier, M, Wolffenbuttel, K, Bokemeyer, C, Li, Y, Lau, Y-FC, Oosterhuis, JW & Looijenga, LHJ 2005, 'Identification of germ cells at risk for neoplastic transformation in gonadoblastoma: an immunohistochemical study for OCT3/4 and TSPY', HUM PATHOL, Jg. 36, Nr. 5, S. 512-21. https://doi.org/10.1016/j.humpath.2005.02.016

APA

Kersemaekers, A-M. F., Honecker, F., Stoop, H., Cools, M., Molier, M., Wolffenbuttel, K., Bokemeyer, C., Li, Y., Lau, Y-F. C., Oosterhuis, J. W., & Looijenga, L. H. J. (2005). Identification of germ cells at risk for neoplastic transformation in gonadoblastoma: an immunohistochemical study for OCT3/4 and TSPY. HUM PATHOL, 36(5), 512-21. https://doi.org/10.1016/j.humpath.2005.02.016

Vancouver

Kersemaekers A-MF, Honecker F, Stoop H, Cools M, Molier M, Wolffenbuttel K et al. Identification of germ cells at risk for neoplastic transformation in gonadoblastoma: an immunohistochemical study for OCT3/4 and TSPY. HUM PATHOL. 2005 Mai;36(5):512-21. https://doi.org/10.1016/j.humpath.2005.02.016

Bibtex

@article{b8daa7c89ca940faaedc9e63428c25d0,
title = "Identification of germ cells at risk for neoplastic transformation in gonadoblastoma: an immunohistochemical study for OCT3/4 and TSPY",
abstract = "Carcinoma in situ (CIS) is the precursor of malignant testicular germ cell tumors (GCTs) of adolescents and young adults, being the neoplastic counterpart of primordial germ cells/gonocytes. Carcinoma in situ cells will develop into invasive seminoma/nonseminoma. Gonadoblastoma (GB) is the precursor of invasive GCTs in dysgenetic gonads, predominantly dysgerminoma (DG). In this process, part of the Y chromosome (GBY region) is involved, for which TSPY is a candidate gene. A detailed immunohistochemical survey was performed for the known diagnostic markers, germ cell/placental alkaline phosphatase (PLAP), c-KIT, and OCT3/4, as well as testis-specific protein on the Y chromosome (TSPY) on a series of GBs, and adjacent invasive DGs. All 5 patients were XY individuals (4 females and 1 male). In contrast to c-KIT, PLAP was positive in all cases. The immature germ cells of GBs were positive for OCT3/4, whereas the mature germ cells were negative for this marker, but positive for TSPY. In every GB, a minor population of germ cells positive for both markers could be identified, similar to most CIS cells and early invasive DG. On progression to an invasive tumor, TSPY can be lost, a process that is also detectable in invasive testicular GCTs compared to CIS. These results indicate that GB is a heterogeneous mix of germ cells, in which the OCT3/4-positive cells have the potential to undergo progression to an invasive tumor. These early invasive stages are initially also positive for TSPY (like CIS), supporting a positive selection mechanism. Therefore, OCT3/4 in combination with TSPY is valuable to identify malignant germ cells in dysgenetic gonads. This could allow better prediction of the risk of progression to a GCT. In addition, the data support the model that GB represents the earliest accessible developmental stage of malignant GCTs.",
keywords = "Adolescent, Adult, Alkaline Phosphatase, Cell Cycle Proteins, Cell Transformation, Neoplastic, DNA-Binding Proteins, Dysgerminoma, Female, GPI-Linked Proteins, Germ Cells, Gonadoblastoma, Humans, Immunohistochemistry, In Situ Hybridization, Fluorescence, Isoenzymes, Male, Nuclear Proteins, Ovarian Neoplasms, Proto-Oncogene Proteins c-kit, Seminoma, Sex-Determining Region Y Protein, Testicular Neoplasms, Transcription Factors, Tumor Markers, Biological",
author = "Kersemaekers, {Anne-Marie F} and Friedemann Honecker and Hans Stoop and Martine Cools and Michel Molier and Katja Wolffenbuttel and Carsten Bokemeyer and Yunmin Li and Lau, {Yun-Fai Chris} and Oosterhuis, {J Wolter} and Looijenga, {Leendert H J}",
year = "2005",
month = may,
doi = "10.1016/j.humpath.2005.02.016",
language = "English",
volume = "36",
pages = "512--21",
journal = "HUM PATHOL",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "5",

}

RIS

TY - JOUR

T1 - Identification of germ cells at risk for neoplastic transformation in gonadoblastoma

T2 - an immunohistochemical study for OCT3/4 and TSPY

AU - Kersemaekers, Anne-Marie F

AU - Honecker, Friedemann

AU - Stoop, Hans

AU - Cools, Martine

AU - Molier, Michel

AU - Wolffenbuttel, Katja

AU - Bokemeyer, Carsten

AU - Li, Yunmin

AU - Lau, Yun-Fai Chris

AU - Oosterhuis, J Wolter

AU - Looijenga, Leendert H J

PY - 2005/5

Y1 - 2005/5

N2 - Carcinoma in situ (CIS) is the precursor of malignant testicular germ cell tumors (GCTs) of adolescents and young adults, being the neoplastic counterpart of primordial germ cells/gonocytes. Carcinoma in situ cells will develop into invasive seminoma/nonseminoma. Gonadoblastoma (GB) is the precursor of invasive GCTs in dysgenetic gonads, predominantly dysgerminoma (DG). In this process, part of the Y chromosome (GBY region) is involved, for which TSPY is a candidate gene. A detailed immunohistochemical survey was performed for the known diagnostic markers, germ cell/placental alkaline phosphatase (PLAP), c-KIT, and OCT3/4, as well as testis-specific protein on the Y chromosome (TSPY) on a series of GBs, and adjacent invasive DGs. All 5 patients were XY individuals (4 females and 1 male). In contrast to c-KIT, PLAP was positive in all cases. The immature germ cells of GBs were positive for OCT3/4, whereas the mature germ cells were negative for this marker, but positive for TSPY. In every GB, a minor population of germ cells positive for both markers could be identified, similar to most CIS cells and early invasive DG. On progression to an invasive tumor, TSPY can be lost, a process that is also detectable in invasive testicular GCTs compared to CIS. These results indicate that GB is a heterogeneous mix of germ cells, in which the OCT3/4-positive cells have the potential to undergo progression to an invasive tumor. These early invasive stages are initially also positive for TSPY (like CIS), supporting a positive selection mechanism. Therefore, OCT3/4 in combination with TSPY is valuable to identify malignant germ cells in dysgenetic gonads. This could allow better prediction of the risk of progression to a GCT. In addition, the data support the model that GB represents the earliest accessible developmental stage of malignant GCTs.

AB - Carcinoma in situ (CIS) is the precursor of malignant testicular germ cell tumors (GCTs) of adolescents and young adults, being the neoplastic counterpart of primordial germ cells/gonocytes. Carcinoma in situ cells will develop into invasive seminoma/nonseminoma. Gonadoblastoma (GB) is the precursor of invasive GCTs in dysgenetic gonads, predominantly dysgerminoma (DG). In this process, part of the Y chromosome (GBY region) is involved, for which TSPY is a candidate gene. A detailed immunohistochemical survey was performed for the known diagnostic markers, germ cell/placental alkaline phosphatase (PLAP), c-KIT, and OCT3/4, as well as testis-specific protein on the Y chromosome (TSPY) on a series of GBs, and adjacent invasive DGs. All 5 patients were XY individuals (4 females and 1 male). In contrast to c-KIT, PLAP was positive in all cases. The immature germ cells of GBs were positive for OCT3/4, whereas the mature germ cells were negative for this marker, but positive for TSPY. In every GB, a minor population of germ cells positive for both markers could be identified, similar to most CIS cells and early invasive DG. On progression to an invasive tumor, TSPY can be lost, a process that is also detectable in invasive testicular GCTs compared to CIS. These results indicate that GB is a heterogeneous mix of germ cells, in which the OCT3/4-positive cells have the potential to undergo progression to an invasive tumor. These early invasive stages are initially also positive for TSPY (like CIS), supporting a positive selection mechanism. Therefore, OCT3/4 in combination with TSPY is valuable to identify malignant germ cells in dysgenetic gonads. This could allow better prediction of the risk of progression to a GCT. In addition, the data support the model that GB represents the earliest accessible developmental stage of malignant GCTs.

KW - Adolescent

KW - Adult

KW - Alkaline Phosphatase

KW - Cell Cycle Proteins

KW - Cell Transformation, Neoplastic

KW - DNA-Binding Proteins

KW - Dysgerminoma

KW - Female

KW - GPI-Linked Proteins

KW - Germ Cells

KW - Gonadoblastoma

KW - Humans

KW - Immunohistochemistry

KW - In Situ Hybridization, Fluorescence

KW - Isoenzymes

KW - Male

KW - Nuclear Proteins

KW - Ovarian Neoplasms

KW - Proto-Oncogene Proteins c-kit

KW - Seminoma

KW - Sex-Determining Region Y Protein

KW - Testicular Neoplasms

KW - Transcription Factors

KW - Tumor Markers, Biological

U2 - 10.1016/j.humpath.2005.02.016

DO - 10.1016/j.humpath.2005.02.016

M3 - SCORING: Journal article

C2 - 15948118

VL - 36

SP - 512

EP - 521

JO - HUM PATHOL

JF - HUM PATHOL

SN - 0046-8177

IS - 5

ER -