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Identification of dominant optimal HLA-B60- and HLA-B61-restricted cytotoxic T-lymphocyte (CTL) epitopes

Standard

Identification of dominant optimal HLA-B60- and HLA-B61-restricted cytotoxic T-lymphocyte (CTL) epitopes : rapid characterization of CTL responses by enzyme-linked immunospot assay. / Altfeld, M A; Trocha, A; Eldridge, R L; Rosenberg, E S; Phillips, M N; Addo, M M; Sekaly, R P; Kalams, S A; Burchett, S A; McIntosh, K; Walker, B D; Goulder, P J.

in: J VIROL, Jahrgang 74, Nr. 18, 09.2000, S. 8541-9.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Altfeld, MA, Trocha, A, Eldridge, RL, Rosenberg, ES, Phillips, MN, Addo, MM, Sekaly, RP, Kalams, SA, Burchett, SA, McIntosh, K, Walker, BD & Goulder, PJ 2000, 'Identification of dominant optimal HLA-B60- and HLA-B61-restricted cytotoxic T-lymphocyte (CTL) epitopes: rapid characterization of CTL responses by enzyme-linked immunospot assay', J VIROL, Jg. 74, Nr. 18, S. 8541-9. https://doi.org/10.1128/jvi.74.18.8541-8549.2000

APA

Altfeld, M. A., Trocha, A., Eldridge, R. L., Rosenberg, E. S., Phillips, M. N., Addo, M. M., Sekaly, R. P., Kalams, S. A., Burchett, S. A., McIntosh, K., Walker, B. D., & Goulder, P. J. (2000). Identification of dominant optimal HLA-B60- and HLA-B61-restricted cytotoxic T-lymphocyte (CTL) epitopes: rapid characterization of CTL responses by enzyme-linked immunospot assay. J VIROL, 74(18), 8541-9. https://doi.org/10.1128/jvi.74.18.8541-8549.2000

Vancouver

Altfeld MA, Trocha A, Eldridge RL, Rosenberg ES, Phillips MN, Addo MM et al. Identification of dominant optimal HLA-B60- and HLA-B61-restricted cytotoxic T-lymphocyte (CTL) epitopes: rapid characterization of CTL responses by enzyme-linked immunospot assay. J VIROL. 2000 Sep;74(18):8541-9. https://doi.org/10.1128/jvi.74.18.8541-8549.2000

Bibtex

@article{b27aefa81b134ab09d9c6f2722b979bb,
title = "Identification of dominant optimal HLA-B60- and HLA-B61-restricted cytotoxic T-lymphocyte (CTL) epitopes: rapid characterization of CTL responses by enzyme-linked immunospot assay",
abstract = "Human immunodeficiency virus type 1 (HIV-1)-specific cytotoxic T-lymphocyte (CTL) responses play a major role in the antiviral immune response, but the relative contribution of CTL responses restricted by different HLA class I molecules is less well defined. HLA-B60 or the related allele B61 is expressed in 10 to 20% of Caucasoid populations and is even more highly prevalent in Asian populations, but yet no CTL epitopes restricted by these alleles have been defined. Here we report the definition of five novel HLA-B60-restricted HIV-1-specific CTL epitopes, using peripheral blood mononuclear cells in enzyme-linked immunospot (Elispot) assays and using CTL clones and lines in cytolytic assays. The dominant HLA-B60-restricted epitope, Nef peptide KEKGGLEGL, was targeted by all eight subjects with B60 and also by both subjects with B61 studied. This study additionally establishes the utility of the Elispot assay as a more rapid and efficient method of defining novel CTL epitopes. This approach will help to define new CTL epitopes that may play an important role in the immune control of HIV-1.",
keywords = "Adult, Amino Acid Sequence, Child, Epitopes, Gene Products, gag/immunology, Gene Products, nef/immunology, HIV Antigens/immunology, HIV Core Protein p24/immunology, HIV Envelope Protein gp41/immunology, HIV-1/immunology, HLA-B Antigens/immunology, Histocompatibility Testing, Humans, Immunoenzyme Techniques/methods, Molecular Sequence Data, T-Lymphocytes, Cytotoxic/immunology, Viral Proteins, gag Gene Products, Human Immunodeficiency Virus, nef Gene Products, Human Immunodeficiency Virus",
author = "Altfeld, {M A} and A Trocha and Eldridge, {R L} and Rosenberg, {E S} and Phillips, {M N} and Addo, {M M} and Sekaly, {R P} and Kalams, {S A} and Burchett, {S A} and K McIntosh and Walker, {B D} and Goulder, {P J}",
year = "2000",
month = sep,
doi = "10.1128/jvi.74.18.8541-8549.2000",
language = "English",
volume = "74",
pages = "8541--9",
journal = "J VIROL",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "18",

}

RIS

TY - JOUR

T1 - Identification of dominant optimal HLA-B60- and HLA-B61-restricted cytotoxic T-lymphocyte (CTL) epitopes

T2 - rapid characterization of CTL responses by enzyme-linked immunospot assay

AU - Altfeld, M A

AU - Trocha, A

AU - Eldridge, R L

AU - Rosenberg, E S

AU - Phillips, M N

AU - Addo, M M

AU - Sekaly, R P

AU - Kalams, S A

AU - Burchett, S A

AU - McIntosh, K

AU - Walker, B D

AU - Goulder, P J

PY - 2000/9

Y1 - 2000/9

N2 - Human immunodeficiency virus type 1 (HIV-1)-specific cytotoxic T-lymphocyte (CTL) responses play a major role in the antiviral immune response, but the relative contribution of CTL responses restricted by different HLA class I molecules is less well defined. HLA-B60 or the related allele B61 is expressed in 10 to 20% of Caucasoid populations and is even more highly prevalent in Asian populations, but yet no CTL epitopes restricted by these alleles have been defined. Here we report the definition of five novel HLA-B60-restricted HIV-1-specific CTL epitopes, using peripheral blood mononuclear cells in enzyme-linked immunospot (Elispot) assays and using CTL clones and lines in cytolytic assays. The dominant HLA-B60-restricted epitope, Nef peptide KEKGGLEGL, was targeted by all eight subjects with B60 and also by both subjects with B61 studied. This study additionally establishes the utility of the Elispot assay as a more rapid and efficient method of defining novel CTL epitopes. This approach will help to define new CTL epitopes that may play an important role in the immune control of HIV-1.

AB - Human immunodeficiency virus type 1 (HIV-1)-specific cytotoxic T-lymphocyte (CTL) responses play a major role in the antiviral immune response, but the relative contribution of CTL responses restricted by different HLA class I molecules is less well defined. HLA-B60 or the related allele B61 is expressed in 10 to 20% of Caucasoid populations and is even more highly prevalent in Asian populations, but yet no CTL epitopes restricted by these alleles have been defined. Here we report the definition of five novel HLA-B60-restricted HIV-1-specific CTL epitopes, using peripheral blood mononuclear cells in enzyme-linked immunospot (Elispot) assays and using CTL clones and lines in cytolytic assays. The dominant HLA-B60-restricted epitope, Nef peptide KEKGGLEGL, was targeted by all eight subjects with B60 and also by both subjects with B61 studied. This study additionally establishes the utility of the Elispot assay as a more rapid and efficient method of defining novel CTL epitopes. This approach will help to define new CTL epitopes that may play an important role in the immune control of HIV-1.

KW - Adult

KW - Amino Acid Sequence

KW - Child

KW - Epitopes

KW - Gene Products, gag/immunology

KW - Gene Products, nef/immunology

KW - HIV Antigens/immunology

KW - HIV Core Protein p24/immunology

KW - HIV Envelope Protein gp41/immunology

KW - HIV-1/immunology

KW - HLA-B Antigens/immunology

KW - Histocompatibility Testing

KW - Humans

KW - Immunoenzyme Techniques/methods

KW - Molecular Sequence Data

KW - T-Lymphocytes, Cytotoxic/immunology

KW - Viral Proteins

KW - gag Gene Products, Human Immunodeficiency Virus

KW - nef Gene Products, Human Immunodeficiency Virus

U2 - 10.1128/jvi.74.18.8541-8549.2000

DO - 10.1128/jvi.74.18.8541-8549.2000

M3 - SCORING: Journal article

C2 - 10954555

VL - 74

SP - 8541

EP - 8549

JO - J VIROL

JF - J VIROL

SN - 0022-538X

IS - 18

ER -