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Identification and characterization of adenosine 5'-tetraphosphate in human myocardial tissue

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Identification and characterization of adenosine 5'-tetraphosphate in human myocardial tissue. / Westhoff, Timm; Jankowski, Joachim; Schmidt, Sven; Luo, Jiankai; Giebing, Günter; Schlüter, Hartmut; Tepel, Martin; Zidek, Walter; van der Giet, Markus.

in: J BIOL CHEM, Jahrgang 278, Nr. 20, 16.05.2003, S. 17735-40.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Westhoff, T, Jankowski, J, Schmidt, S, Luo, J, Giebing, G, Schlüter, H, Tepel, M, Zidek, W & van der Giet, M 2003, 'Identification and characterization of adenosine 5'-tetraphosphate in human myocardial tissue', J BIOL CHEM, Jg. 278, Nr. 20, S. 17735-40. https://doi.org/10.1074/jbc.M300288200

APA

Westhoff, T., Jankowski, J., Schmidt, S., Luo, J., Giebing, G., Schlüter, H., Tepel, M., Zidek, W., & van der Giet, M. (2003). Identification and characterization of adenosine 5'-tetraphosphate in human myocardial tissue. J BIOL CHEM, 278(20), 17735-40. https://doi.org/10.1074/jbc.M300288200

Vancouver

Westhoff T, Jankowski J, Schmidt S, Luo J, Giebing G, Schlüter H et al. Identification and characterization of adenosine 5'-tetraphosphate in human myocardial tissue. J BIOL CHEM. 2003 Mai 16;278(20):17735-40. https://doi.org/10.1074/jbc.M300288200

Bibtex

@article{6c4252133f194acd9e9557cdca331524,
title = "Identification and characterization of adenosine 5'-tetraphosphate in human myocardial tissue",
abstract = "Endocrine functions of the human heart have been studied extensively. Only recently, nucleotidergic mechanisms have been studied in detail. Therefore, an isolation strategy was developed to isolate novel nucleotide compounds from human myocardium. The human myocardial tissue was fractionated by several chromatographic studies. A substance purified to homogeneity was identified as adenosine 5'-tetraphosphate (Ap(4)) by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS), post-source decay MALDI MS, and enzymatic cleavage analysis. Furthermore, Ap(4) was also identified in ventricular specific granules. In the isolated perfused rat heart, Ap(4) elicited dose-dependent vasodilations. Vasodilator responses were abolished in the presence of the P(2Y1) receptor antagonist MRS 2179 (1 microm) or the NO synthase inhibitor N(G)-nitro-l-arginine methyl ester (50 microm). After removal of the endothelium by Triton X-100, Ap(4) induced dose-dependent vasoconstrictions. Inhibition of P(2X) receptors by pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid (30 microm) or desensitization of P(2X) receptors by alpha,beta-methylene ATP (alpha,beta-meATP, 1 microm) diminished these vasoconstrictor responses completely. In the present study Ap(4) has been isolated from human tissue. Ap(4) was shown to exist in human myocardial tissue and was identified in ventricular specific granules. In coronary vasculature the nucleotide exerted vasodilation via endothelial P(2Y1) receptors and vasoconstriction via P(2X) receptors on vascular smooth muscle cells. Ap(4) acts as an endogenous extracellular mediator and might contribute to the regulation of coronary perfusion.",
keywords = "Adenine Nucleotides, Animals, Detergents, Endothelium, Vascular, Humans, Male, Myocardium, Octoxynol, Perfusion, Rats, Rats, Inbred WKY, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Swine, Time Factors, Ultraviolet Rays, Vasoconstrictor Agents, Journal Article",
author = "Timm Westhoff and Joachim Jankowski and Sven Schmidt and Jiankai Luo and G{\"u}nter Giebing and Hartmut Schl{\"u}ter and Martin Tepel and Walter Zidek and {van der Giet}, Markus",
year = "2003",
month = may,
day = "16",
doi = "10.1074/jbc.M300288200",
language = "English",
volume = "278",
pages = "17735--40",
journal = "J BIOL CHEM",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "20",

}

RIS

TY - JOUR

T1 - Identification and characterization of adenosine 5'-tetraphosphate in human myocardial tissue

AU - Westhoff, Timm

AU - Jankowski, Joachim

AU - Schmidt, Sven

AU - Luo, Jiankai

AU - Giebing, Günter

AU - Schlüter, Hartmut

AU - Tepel, Martin

AU - Zidek, Walter

AU - van der Giet, Markus

PY - 2003/5/16

Y1 - 2003/5/16

N2 - Endocrine functions of the human heart have been studied extensively. Only recently, nucleotidergic mechanisms have been studied in detail. Therefore, an isolation strategy was developed to isolate novel nucleotide compounds from human myocardium. The human myocardial tissue was fractionated by several chromatographic studies. A substance purified to homogeneity was identified as adenosine 5'-tetraphosphate (Ap(4)) by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS), post-source decay MALDI MS, and enzymatic cleavage analysis. Furthermore, Ap(4) was also identified in ventricular specific granules. In the isolated perfused rat heart, Ap(4) elicited dose-dependent vasodilations. Vasodilator responses were abolished in the presence of the P(2Y1) receptor antagonist MRS 2179 (1 microm) or the NO synthase inhibitor N(G)-nitro-l-arginine methyl ester (50 microm). After removal of the endothelium by Triton X-100, Ap(4) induced dose-dependent vasoconstrictions. Inhibition of P(2X) receptors by pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid (30 microm) or desensitization of P(2X) receptors by alpha,beta-methylene ATP (alpha,beta-meATP, 1 microm) diminished these vasoconstrictor responses completely. In the present study Ap(4) has been isolated from human tissue. Ap(4) was shown to exist in human myocardial tissue and was identified in ventricular specific granules. In coronary vasculature the nucleotide exerted vasodilation via endothelial P(2Y1) receptors and vasoconstriction via P(2X) receptors on vascular smooth muscle cells. Ap(4) acts as an endogenous extracellular mediator and might contribute to the regulation of coronary perfusion.

AB - Endocrine functions of the human heart have been studied extensively. Only recently, nucleotidergic mechanisms have been studied in detail. Therefore, an isolation strategy was developed to isolate novel nucleotide compounds from human myocardium. The human myocardial tissue was fractionated by several chromatographic studies. A substance purified to homogeneity was identified as adenosine 5'-tetraphosphate (Ap(4)) by matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS), post-source decay MALDI MS, and enzymatic cleavage analysis. Furthermore, Ap(4) was also identified in ventricular specific granules. In the isolated perfused rat heart, Ap(4) elicited dose-dependent vasodilations. Vasodilator responses were abolished in the presence of the P(2Y1) receptor antagonist MRS 2179 (1 microm) or the NO synthase inhibitor N(G)-nitro-l-arginine methyl ester (50 microm). After removal of the endothelium by Triton X-100, Ap(4) induced dose-dependent vasoconstrictions. Inhibition of P(2X) receptors by pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid (30 microm) or desensitization of P(2X) receptors by alpha,beta-methylene ATP (alpha,beta-meATP, 1 microm) diminished these vasoconstrictor responses completely. In the present study Ap(4) has been isolated from human tissue. Ap(4) was shown to exist in human myocardial tissue and was identified in ventricular specific granules. In coronary vasculature the nucleotide exerted vasodilation via endothelial P(2Y1) receptors and vasoconstriction via P(2X) receptors on vascular smooth muscle cells. Ap(4) acts as an endogenous extracellular mediator and might contribute to the regulation of coronary perfusion.

KW - Adenine Nucleotides

KW - Animals

KW - Detergents

KW - Endothelium, Vascular

KW - Humans

KW - Male

KW - Myocardium

KW - Octoxynol

KW - Perfusion

KW - Rats

KW - Rats, Inbred WKY

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Swine

KW - Time Factors

KW - Ultraviolet Rays

KW - Vasoconstrictor Agents

KW - Journal Article

U2 - 10.1074/jbc.M300288200

DO - 10.1074/jbc.M300288200

M3 - SCORING: Journal article

C2 - 12609994

VL - 278

SP - 17735

EP - 17740

JO - J BIOL CHEM

JF - J BIOL CHEM

SN - 0021-9258

IS - 20

ER -