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Hypothermia Promotes Survival of Ischemic Retinal Ganglion Cells

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Hypothermia Promotes Survival of Ischemic Retinal Ganglion Cells. / Reinhard, Katja; Mutter, Marion; Gustafsson, Elisabeth; Gustafsson, Leon; Vaegler, Martin; Schultheiss, Maximilian; Müller, Sebastian; Yoeruek, Efdal; Schrader, Merle; Münch, Thomas A.

in: INVEST OPHTH VIS SCI, Jahrgang 57, Nr. 2, 02.2016, S. 658-63.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Reinhard, K, Mutter, M, Gustafsson, E, Gustafsson, L, Vaegler, M, Schultheiss, M, Müller, S, Yoeruek, E, Schrader, M & Münch, TA 2016, 'Hypothermia Promotes Survival of Ischemic Retinal Ganglion Cells', INVEST OPHTH VIS SCI, Jg. 57, Nr. 2, S. 658-63. https://doi.org/10.1167/iovs.15-17751

APA

Reinhard, K., Mutter, M., Gustafsson, E., Gustafsson, L., Vaegler, M., Schultheiss, M., Müller, S., Yoeruek, E., Schrader, M., & Münch, T. A. (2016). Hypothermia Promotes Survival of Ischemic Retinal Ganglion Cells. INVEST OPHTH VIS SCI, 57(2), 658-63. https://doi.org/10.1167/iovs.15-17751

Vancouver

Reinhard K, Mutter M, Gustafsson E, Gustafsson L, Vaegler M, Schultheiss M et al. Hypothermia Promotes Survival of Ischemic Retinal Ganglion Cells. INVEST OPHTH VIS SCI. 2016 Feb;57(2):658-63. https://doi.org/10.1167/iovs.15-17751

Bibtex

@article{72a488f5bd824129abcea1f938982488,
title = "Hypothermia Promotes Survival of Ischemic Retinal Ganglion Cells",
abstract = "PURPOSE: Ischemic stroke in retinal arteries leads to death of neural tissue and ultimately to blindness. The retina is known to die within 4 hours after onset of ischemia. It is debated whether hypothermia might increase the time window for medical treatment and thereby the chance of recovering sight. In order to characterize the time course of cell death during ischemia and potential beneficial effects of hypothermia in more detail, we investigated the survival of ganglion cells in ischemic pig and human retina as a function of time and temperature.METHODS: Eyes were obtained from minipigs and from human donors post mortem. Enucleated minipig eyes were stored for defined durations at three different temperatures (37 °C, 21 °C, and 4 °C). In order to assess the viability of the tissue, we measured ganglion cell activity (spiking) with multielectrode arrays.RESULTS: Minipig retinal ganglion cell function was severely compromised after 2 hours of ischemia at body temperature. After 4 hours, ganglion cells did not fire action potentials anymore. However, at 21 °C, ganglion cell activity was maintained under ischemic conditions for up to 12 hours, and for at least 50 hours at 4 °C. In postmortem human retina, we recorded ganglion cell activity in retinas received up to 27 hours after death.CONCLUSIONS: Our results demonstrate that hypothermia greatly increases survival of retinal ganglion cells exposed to ischemia. These results might be relevant for the future treatment of retinal ischemia.",
keywords = "Animals, Cadaver, Cell Count, Cell Death, Cell Survival, Disease Models, Animal, Humans, Hypothermia, Induced, Ischemia, Retinal Diseases, Retinal Ganglion Cells, Swine, Swine, Miniature, Journal Article, Research Support, Non-U.S. Gov't",
author = "Katja Reinhard and Marion Mutter and Elisabeth Gustafsson and Leon Gustafsson and Martin Vaegler and Maximilian Schultheiss and Sebastian M{\"u}ller and Efdal Yoeruek and Merle Schrader and M{\"u}nch, {Thomas A}",
year = "2016",
month = feb,
doi = "10.1167/iovs.15-17751",
language = "English",
volume = "57",
pages = "658--63",
journal = "INVEST OPHTH VIS SCI",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Hypothermia Promotes Survival of Ischemic Retinal Ganglion Cells

AU - Reinhard, Katja

AU - Mutter, Marion

AU - Gustafsson, Elisabeth

AU - Gustafsson, Leon

AU - Vaegler, Martin

AU - Schultheiss, Maximilian

AU - Müller, Sebastian

AU - Yoeruek, Efdal

AU - Schrader, Merle

AU - Münch, Thomas A

PY - 2016/2

Y1 - 2016/2

N2 - PURPOSE: Ischemic stroke in retinal arteries leads to death of neural tissue and ultimately to blindness. The retina is known to die within 4 hours after onset of ischemia. It is debated whether hypothermia might increase the time window for medical treatment and thereby the chance of recovering sight. In order to characterize the time course of cell death during ischemia and potential beneficial effects of hypothermia in more detail, we investigated the survival of ganglion cells in ischemic pig and human retina as a function of time and temperature.METHODS: Eyes were obtained from minipigs and from human donors post mortem. Enucleated minipig eyes were stored for defined durations at three different temperatures (37 °C, 21 °C, and 4 °C). In order to assess the viability of the tissue, we measured ganglion cell activity (spiking) with multielectrode arrays.RESULTS: Minipig retinal ganglion cell function was severely compromised after 2 hours of ischemia at body temperature. After 4 hours, ganglion cells did not fire action potentials anymore. However, at 21 °C, ganglion cell activity was maintained under ischemic conditions for up to 12 hours, and for at least 50 hours at 4 °C. In postmortem human retina, we recorded ganglion cell activity in retinas received up to 27 hours after death.CONCLUSIONS: Our results demonstrate that hypothermia greatly increases survival of retinal ganglion cells exposed to ischemia. These results might be relevant for the future treatment of retinal ischemia.

AB - PURPOSE: Ischemic stroke in retinal arteries leads to death of neural tissue and ultimately to blindness. The retina is known to die within 4 hours after onset of ischemia. It is debated whether hypothermia might increase the time window for medical treatment and thereby the chance of recovering sight. In order to characterize the time course of cell death during ischemia and potential beneficial effects of hypothermia in more detail, we investigated the survival of ganglion cells in ischemic pig and human retina as a function of time and temperature.METHODS: Eyes were obtained from minipigs and from human donors post mortem. Enucleated minipig eyes were stored for defined durations at three different temperatures (37 °C, 21 °C, and 4 °C). In order to assess the viability of the tissue, we measured ganglion cell activity (spiking) with multielectrode arrays.RESULTS: Minipig retinal ganglion cell function was severely compromised after 2 hours of ischemia at body temperature. After 4 hours, ganglion cells did not fire action potentials anymore. However, at 21 °C, ganglion cell activity was maintained under ischemic conditions for up to 12 hours, and for at least 50 hours at 4 °C. In postmortem human retina, we recorded ganglion cell activity in retinas received up to 27 hours after death.CONCLUSIONS: Our results demonstrate that hypothermia greatly increases survival of retinal ganglion cells exposed to ischemia. These results might be relevant for the future treatment of retinal ischemia.

KW - Animals

KW - Cadaver

KW - Cell Count

KW - Cell Death

KW - Cell Survival

KW - Disease Models, Animal

KW - Humans

KW - Hypothermia, Induced

KW - Ischemia

KW - Retinal Diseases

KW - Retinal Ganglion Cells

KW - Swine

KW - Swine, Miniature

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1167/iovs.15-17751

DO - 10.1167/iovs.15-17751

M3 - SCORING: Journal article

C2 - 26903226

VL - 57

SP - 658

EP - 663

JO - INVEST OPHTH VIS SCI

JF - INVEST OPHTH VIS SCI

SN - 0146-0404

IS - 2

ER -