Human Th1 and Th2 lymphocytes are distinguished by calcium flux regulation during the first 10 min of lymphocyte activation.
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Human Th1 and Th2 lymphocytes are distinguished by calcium flux regulation during the first 10 min of lymphocyte activation. / Toldi, Gergely; Kaposi, Ambrus; Zsembery, Ákos; Treszl, András; Tulassay, Tivadar; Vásárhelyi, Barna.
in: IMMUNOBIOLOGY, Jahrgang 217, Nr. 1, 1, 2012, S. 37-43.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Human Th1 and Th2 lymphocytes are distinguished by calcium flux regulation during the first 10 min of lymphocyte activation.
AU - Toldi, Gergely
AU - Kaposi, Ambrus
AU - Zsembery, Ákos
AU - Treszl, András
AU - Tulassay, Tivadar
AU - Vásárhelyi, Barna
PY - 2012
Y1 - 2012
N2 - Preliminary data suggest different intracellular calcium handling of Th1 and Th2 lymphocytes that may contribute to distinct cytokine production patterns. In this study we explored the contribution of the main mechanisms in charge of the elevation and decrease of cytoplasmic free calcium levels, i.e., the endoplasmic calcium release, the calcium release activated calcium (CRAC) channel, the mitochondrial calcium uniporter (MCU), the sarco/endoplasmic reticulum calcium ATPase (SERCA), and the plasma membrane calcium ATPase (PMCA) during the first 10 min of activation in human Th1 and Th2 lymphocytes applying a kinetic flow cytometry approach. We isolated peripheral blood mononuclear cells from 10 healthy individuals. Cells were stained with CD4, CXCR3 and CCR4 cell surface markers to identify Th1 and Th2 cells, respectively and loaded with Fluo-3/AM calcium sensitive dye. Cells were activated with phytohemagglutinine and alterations of cytoplasmic free calcium levels were monitored for 10 min after specific inhibition of the above mechanisms. Our results revealed delicate differences in calcium flux kinetics of Th1 and Th2 lymphocytes. The lower activity of MCU, and therefore of CRAC channels, along with the higher activity of the SERCA pump account for the notion that Th2 cells go through a lower level of lymphocyte activation compared with Th1 cells upon identical activating stimuli. The observed differences in calcium flux of Th1 and Th2 cells may contribute to different calcium handling kinetics and, hence, to distinct cytokine production patterns by these subsets.
AB - Preliminary data suggest different intracellular calcium handling of Th1 and Th2 lymphocytes that may contribute to distinct cytokine production patterns. In this study we explored the contribution of the main mechanisms in charge of the elevation and decrease of cytoplasmic free calcium levels, i.e., the endoplasmic calcium release, the calcium release activated calcium (CRAC) channel, the mitochondrial calcium uniporter (MCU), the sarco/endoplasmic reticulum calcium ATPase (SERCA), and the plasma membrane calcium ATPase (PMCA) during the first 10 min of activation in human Th1 and Th2 lymphocytes applying a kinetic flow cytometry approach. We isolated peripheral blood mononuclear cells from 10 healthy individuals. Cells were stained with CD4, CXCR3 and CCR4 cell surface markers to identify Th1 and Th2 cells, respectively and loaded with Fluo-3/AM calcium sensitive dye. Cells were activated with phytohemagglutinine and alterations of cytoplasmic free calcium levels were monitored for 10 min after specific inhibition of the above mechanisms. Our results revealed delicate differences in calcium flux kinetics of Th1 and Th2 lymphocytes. The lower activity of MCU, and therefore of CRAC channels, along with the higher activity of the SERCA pump account for the notion that Th2 cells go through a lower level of lymphocyte activation compared with Th1 cells upon identical activating stimuli. The observed differences in calcium flux of Th1 and Th2 cells may contribute to different calcium handling kinetics and, hence, to distinct cytokine production patterns by these subsets.
KW - Adult
KW - Humans
KW - Male
KW - Female
KW - Kinetics
KW - Flow Cytometry
KW - Biological Markers/metabolism
KW - Calcium/metabolism
KW - Aniline Compounds/analysis
KW - Calcium Channels/immunology/metabolism
KW - Calcium Signaling/immunology
KW - Cytokines/biosynthesis/immunology
KW - Cytosol/immunology/metabolism
KW - Immunity, Cellular
KW - Lymphocyte Activation/drug effects/immunology
KW - Phytohemagglutinins/pharmacology
KW - Plasma Membrane Calcium-Transporting ATPases/immunology/metabolism
KW - Sarcoplasmic Reticulum Calcium-Transporting ATPases/immunology/metabolism
KW - Th1 Cells/cytology/immunology/metabolism
KW - Th2 Cells/cytology/immunology/metabolism
KW - Xanthenes/analysis
KW - Adult
KW - Humans
KW - Male
KW - Female
KW - Kinetics
KW - Flow Cytometry
KW - Biological Markers/metabolism
KW - Calcium/metabolism
KW - Aniline Compounds/analysis
KW - Calcium Channels/immunology/metabolism
KW - Calcium Signaling/immunology
KW - Cytokines/biosynthesis/immunology
KW - Cytosol/immunology/metabolism
KW - Immunity, Cellular
KW - Lymphocyte Activation/drug effects/immunology
KW - Phytohemagglutinins/pharmacology
KW - Plasma Membrane Calcium-Transporting ATPases/immunology/metabolism
KW - Sarcoplasmic Reticulum Calcium-Transporting ATPases/immunology/metabolism
KW - Th1 Cells/cytology/immunology/metabolism
KW - Th2 Cells/cytology/immunology/metabolism
KW - Xanthenes/analysis
M3 - SCORING: Journal article
VL - 217
SP - 37
EP - 43
JO - IMMUNOBIOLOGY
JF - IMMUNOBIOLOGY
SN - 0171-2985
IS - 1
M1 - 1
ER -
