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Human serum substitution by artificial sera of scalable allergen reactivity based on polyclonal antibodies and chimeras of human FcγRI and IgE domains

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Human serum substitution by artificial sera of scalable allergen reactivity based on polyclonal antibodies and chimeras of human FcγRI and IgE domains. / Offermann, N; Plum, M; Hübner, U; Rathloff, K; Braren, I; Fooke, M; Spillner, E.

in: ALLERGY, Jahrgang 71, Nr. 12, 12.2016, S. 1794-1799.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Offermann, N, Plum, M, Hübner, U, Rathloff, K, Braren, I, Fooke, M & Spillner, E 2016, 'Human serum substitution by artificial sera of scalable allergen reactivity based on polyclonal antibodies and chimeras of human FcγRI and IgE domains', ALLERGY, Jg. 71, Nr. 12, S. 1794-1799. https://doi.org/10.1111/all.13038

APA

Offermann, N., Plum, M., Hübner, U., Rathloff, K., Braren, I., Fooke, M., & Spillner, E. (2016). Human serum substitution by artificial sera of scalable allergen reactivity based on polyclonal antibodies and chimeras of human FcγRI and IgE domains. ALLERGY, 71(12), 1794-1799. https://doi.org/10.1111/all.13038

Vancouver

Offermann N, Plum M, Hübner U, Rathloff K, Braren I, Fooke M et al. Human serum substitution by artificial sera of scalable allergen reactivity based on polyclonal antibodies and chimeras of human FcγRI and IgE domains. ALLERGY. 2016 Dez;71(12):1794-1799. https://doi.org/10.1111/all.13038

Bibtex

@article{9cd67d5f1e684d1ea86ae62c74f8856d,
title = "Human serum substitution by artificial sera of scalable allergen reactivity based on polyclonal antibodies and chimeras of human FcγRI and IgE domains",
abstract = "Human sera are the first choice as controls for diagnostic applications such as immunoassays, but are limited regarding availability, varying quality, and high costs. In this study, we aimed to circumvent these limitations by the use of a chimeric adaptor molecule comprising the extracellular domains of the human FcγRI (CD64) fused with human IgE Fc domains (CD64-IgE Fc). Allergen-specific antibodies were produced in rabbits using eight different allergens, extracts, and allergen mixtures including mites, pollen, drugs, and food. Preincubation of polyclonal IgG with CD64-IgE Fc established allergen-specific artificial sera that showed comparable results for more than 20 allergens and allergen extracts in three diagnostic systems for the determination of specific IgE. The agreement for these artificial sera is within ±1 radioallergosorbent test (RAST) class. Hence, rabbit IgG complexed with the IgG-specific CD64-IgE Fc adaptor molecule could provide a substitute for human reference sera with specificity for virtually any protein of interest.",
author = "N Offermann and M Plum and U H{\"u}bner and K Rathloff and I Braren and M Fooke and E Spillner",
note = "{\textcopyright} 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.",
year = "2016",
month = dec,
doi = "10.1111/all.13038",
language = "English",
volume = "71",
pages = "1794--1799",
journal = "ALLERGY",
issn = "0105-4538",
publisher = "Wiley-Blackwell",
number = "12",

}

RIS

TY - JOUR

T1 - Human serum substitution by artificial sera of scalable allergen reactivity based on polyclonal antibodies and chimeras of human FcγRI and IgE domains

AU - Offermann, N

AU - Plum, M

AU - Hübner, U

AU - Rathloff, K

AU - Braren, I

AU - Fooke, M

AU - Spillner, E

N1 - © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

PY - 2016/12

Y1 - 2016/12

N2 - Human sera are the first choice as controls for diagnostic applications such as immunoassays, but are limited regarding availability, varying quality, and high costs. In this study, we aimed to circumvent these limitations by the use of a chimeric adaptor molecule comprising the extracellular domains of the human FcγRI (CD64) fused with human IgE Fc domains (CD64-IgE Fc). Allergen-specific antibodies were produced in rabbits using eight different allergens, extracts, and allergen mixtures including mites, pollen, drugs, and food. Preincubation of polyclonal IgG with CD64-IgE Fc established allergen-specific artificial sera that showed comparable results for more than 20 allergens and allergen extracts in three diagnostic systems for the determination of specific IgE. The agreement for these artificial sera is within ±1 radioallergosorbent test (RAST) class. Hence, rabbit IgG complexed with the IgG-specific CD64-IgE Fc adaptor molecule could provide a substitute for human reference sera with specificity for virtually any protein of interest.

AB - Human sera are the first choice as controls for diagnostic applications such as immunoassays, but are limited regarding availability, varying quality, and high costs. In this study, we aimed to circumvent these limitations by the use of a chimeric adaptor molecule comprising the extracellular domains of the human FcγRI (CD64) fused with human IgE Fc domains (CD64-IgE Fc). Allergen-specific antibodies were produced in rabbits using eight different allergens, extracts, and allergen mixtures including mites, pollen, drugs, and food. Preincubation of polyclonal IgG with CD64-IgE Fc established allergen-specific artificial sera that showed comparable results for more than 20 allergens and allergen extracts in three diagnostic systems for the determination of specific IgE. The agreement for these artificial sera is within ±1 radioallergosorbent test (RAST) class. Hence, rabbit IgG complexed with the IgG-specific CD64-IgE Fc adaptor molecule could provide a substitute for human reference sera with specificity for virtually any protein of interest.

U2 - 10.1111/all.13038

DO - 10.1111/all.13038

M3 - SCORING: Journal article

C2 - 27588368

VL - 71

SP - 1794

EP - 1799

JO - ALLERGY

JF - ALLERGY

SN - 0105-4538

IS - 12

ER -