Human macrophage activation triggered by chitotriosidase-mediated chitin and chitosan degradation

Christian Gorzelanny; Birgit Pöppelmann; Karin Pappelbaum; Bruno M Moerschbacher; Stefan W Schneider

doi:10.1016/j.biomaterials.2010.07.100

Human macrophage activation triggered by chitotriosidase-mediated chitin and chitosan degradation

Standard

Human macrophage activation triggered by chitotriosidase-mediated chitin and chitosan degradation. / Gorzelanny, Christian; Pöppelmann, Birgit; Pappelbaum, Karin; Moerschbacher, Bruno M; Schneider, Stefan W.

in: BIOMATERIALS, Jahrgang 31, Nr. 33, 11.2010, S. 8556-63.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Gorzelanny, C, Pöppelmann, B, Pappelbaum, K, Moerschbacher, BM & Schneider, SW 2010, 'Human macrophage activation triggered by chitotriosidase-mediated chitin and chitosan degradation', BIOMATERIALS, Jg. 31, Nr. 33, S. 8556-63. https://doi.org/10.1016/j.biomaterials.2010.07.100

APA

Gorzelanny, C., Pöppelmann, B., Pappelbaum, K., Moerschbacher, B. M., & Schneider, S. W. (2010). Human macrophage activation triggered by chitotriosidase-mediated chitin and chitosan degradation. BIOMATERIALS, 31(33), 8556-63. https://doi.org/10.1016/j.biomaterials.2010.07.100

Vancouver

Gorzelanny C, Pöppelmann B, Pappelbaum K, Moerschbacher BM, Schneider SW. Human macrophage activation triggered by chitotriosidase-mediated chitin and chitosan degradation. BIOMATERIALS. 2010 Nov;31(33):8556-63. https://doi.org/10.1016/j.biomaterials.2010.07.100

Bibtex

@article{5aababf77bfd44caa4ecd05c3d646e2e,

title = "Human macrophage activation triggered by chitotriosidase-mediated chitin and chitosan degradation",

abstract = "Chitin a biopolymer composed of N-acetyl-D-glucosamine (GlcNAc) residues is a structural component in human pathogens such as nematodes and fungi. Deacetylation of chitin generates chitosan which has been recently found in the cell wall of Cryptococcus neoformans a human pathogenic fungi causing life-threatening meningoencephalitis. While chitin and chitosan are currently studied as compounds of medical devices such as wound dressings or nanoparticles, its immunostimulatory potential and its metabolic fate in humans remains unclear. To gain more fundamental insights on the immunological properties of chitin and chitosan in humans we studied their degradation by chitotriosidase (ChT) and their inflammatory properties on human macrophages. Our data show that chitinhexamer is not able to increase the expression of inflammatory cytokines significantly. However, we measured an induction of ChT secretion upon chitinhexamer treatment. By analysis of human ChT-mediated cleavage of chitosan we could demonstrate a special mechanism of substrate cleavage, defined as processivity. Processivity enables the rapid production of small and diffusible chitin and chitosan fragments. In comparison to large chitosan polymers these ChT-produced small chitin/chitosan oligomers exhibit strongest macrophage activating properties reflected by an enhanced ChT secretion. Here we show that recognition of chitin and chitosan by human macrophages is triggered by the enzyme ChT due to the production of chitin and chitosan oligomers which in turn stimulates further ChT secretion and consequently oligomers production. Finally, we demonstrate that despite the high cooperativity of chitosan and chitin clearance by ChT seems very specific as no inflammatory response could be detected.",

keywords = "Cell Line, Chitin, Chitosan, Computational Biology, Hexosaminidases, Humans, Inflammation, Macrophage Activation, Macrophages, Models, Biological, Journal Article, Research Support, Non-U.S. Gov't",

author = "Christian Gorzelanny and Birgit P{\"o}ppelmann and Karin Pappelbaum and Moerschbacher, {Bruno M} and Schneider, {Stefan W}",

year = "2010",

month = nov,

doi = "10.1016/j.biomaterials.2010.07.100",

language = "English",

volume = "31",

pages = "8556--63",

journal = "BIOMATERIALS",

issn = "0142-9612",

publisher = "Elsevier BV",

number = "33",

}

RIS

TY - JOUR

T1 - Human macrophage activation triggered by chitotriosidase-mediated chitin and chitosan degradation

AU - Gorzelanny, Christian

AU - Pöppelmann, Birgit

AU - Pappelbaum, Karin

AU - Moerschbacher, Bruno M

AU - Schneider, Stefan W

PY - 2010/11

Y1 - 2010/11

N2 - Chitin a biopolymer composed of N-acetyl-D-glucosamine (GlcNAc) residues is a structural component in human pathogens such as nematodes and fungi. Deacetylation of chitin generates chitosan which has been recently found in the cell wall of Cryptococcus neoformans a human pathogenic fungi causing life-threatening meningoencephalitis. While chitin and chitosan are currently studied as compounds of medical devices such as wound dressings or nanoparticles, its immunostimulatory potential and its metabolic fate in humans remains unclear. To gain more fundamental insights on the immunological properties of chitin and chitosan in humans we studied their degradation by chitotriosidase (ChT) and their inflammatory properties on human macrophages. Our data show that chitinhexamer is not able to increase the expression of inflammatory cytokines significantly. However, we measured an induction of ChT secretion upon chitinhexamer treatment. By analysis of human ChT-mediated cleavage of chitosan we could demonstrate a special mechanism of substrate cleavage, defined as processivity. Processivity enables the rapid production of small and diffusible chitin and chitosan fragments. In comparison to large chitosan polymers these ChT-produced small chitin/chitosan oligomers exhibit strongest macrophage activating properties reflected by an enhanced ChT secretion. Here we show that recognition of chitin and chitosan by human macrophages is triggered by the enzyme ChT due to the production of chitin and chitosan oligomers which in turn stimulates further ChT secretion and consequently oligomers production. Finally, we demonstrate that despite the high cooperativity of chitosan and chitin clearance by ChT seems very specific as no inflammatory response could be detected.

AB - Chitin a biopolymer composed of N-acetyl-D-glucosamine (GlcNAc) residues is a structural component in human pathogens such as nematodes and fungi. Deacetylation of chitin generates chitosan which has been recently found in the cell wall of Cryptococcus neoformans a human pathogenic fungi causing life-threatening meningoencephalitis. While chitin and chitosan are currently studied as compounds of medical devices such as wound dressings or nanoparticles, its immunostimulatory potential and its metabolic fate in humans remains unclear. To gain more fundamental insights on the immunological properties of chitin and chitosan in humans we studied their degradation by chitotriosidase (ChT) and their inflammatory properties on human macrophages. Our data show that chitinhexamer is not able to increase the expression of inflammatory cytokines significantly. However, we measured an induction of ChT secretion upon chitinhexamer treatment. By analysis of human ChT-mediated cleavage of chitosan we could demonstrate a special mechanism of substrate cleavage, defined as processivity. Processivity enables the rapid production of small and diffusible chitin and chitosan fragments. In comparison to large chitosan polymers these ChT-produced small chitin/chitosan oligomers exhibit strongest macrophage activating properties reflected by an enhanced ChT secretion. Here we show that recognition of chitin and chitosan by human macrophages is triggered by the enzyme ChT due to the production of chitin and chitosan oligomers which in turn stimulates further ChT secretion and consequently oligomers production. Finally, we demonstrate that despite the high cooperativity of chitosan and chitin clearance by ChT seems very specific as no inflammatory response could be detected.

KW - Cell Line

KW - Chitin

KW - Chitosan

KW - Computational Biology

KW - Hexosaminidases

KW - Humans

KW - Inflammation

KW - Macrophage Activation

KW - Macrophages

KW - Models, Biological

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.biomaterials.2010.07.100

DO - 10.1016/j.biomaterials.2010.07.100

M3 - SCORING: Journal article

C2 - 20797781

VL - 31

SP - 8556

EP - 8563

JO - BIOMATERIALS

JF - BIOMATERIALS

SN - 0142-9612

IS - 33

ER -