High detection rate of Rickettsia africae in Amblyomma variegatum but low prevalence of anti-rickettsial antibodies in healthy pregnant women in Madagascar

Christian Keller; Andreas Krüger; Norbert Georg Schwarz; Raphael Rakotozandrindrainy; Jean Philibert Rakotondrainiarivelo; Tsiry Razafindrabe; Henri Derschum; Cornelia Silaghi; Daniela Pothmann; Alexandra Veit; Benedikt Hogan; Jürgen May; Mirko Girmann; Stefanie Kramme; Bernhard Fleischer; Sven Poppert

doi:10.1016/j.ttbdis.2015.08.005

High detection rate of Rickettsia africae in Amblyomma variegatum but low prevalence of anti-rickettsial antibodies in healthy pregnant women in Madagascar

Standard

High detection rate of Rickettsia africae in Amblyomma variegatum but low prevalence of anti-rickettsial antibodies in healthy pregnant women in Madagascar. / Keller, Christian; Krüger, Andreas; Schwarz, Norbert Georg; Rakotozandrindrainy, Raphael; Rakotondrainiarivelo, Jean Philibert; Razafindrabe, Tsiry; Derschum, Henri; Silaghi, Cornelia; Pothmann, Daniela; Veit, Alexandra; Hogan, Benedikt; May, Jürgen; Girmann, Mirko; Kramme, Stefanie; Fleischer, Bernhard; Poppert, Sven.

in: TICKS TICK-BORNE DIS, Jahrgang 7, Nr. 1, 02.2016, S. 60-5.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Keller, C, Krüger, A, Schwarz, NG, Rakotozandrindrainy, R, Rakotondrainiarivelo, JP, Razafindrabe, T, Derschum, H, Silaghi, C, Pothmann, D, Veit, A, Hogan, B, May, J, Girmann, M, Kramme, S, Fleischer, B & Poppert, S 2016, 'High detection rate of Rickettsia africae in Amblyomma variegatum but low prevalence of anti-rickettsial antibodies in healthy pregnant women in Madagascar', TICKS TICK-BORNE DIS, Jg. 7, Nr. 1, S. 60-5. https://doi.org/10.1016/j.ttbdis.2015.08.005

APA

Keller, C., Krüger, A., Schwarz, N. G., Rakotozandrindrainy, R., Rakotondrainiarivelo, J. P., Razafindrabe, T., Derschum, H., Silaghi, C., Pothmann, D., Veit, A., Hogan, B., May, J., Girmann, M., Kramme, S., Fleischer, B., & Poppert, S. (2016). High detection rate of Rickettsia africae in Amblyomma variegatum but low prevalence of anti-rickettsial antibodies in healthy pregnant women in Madagascar. TICKS TICK-BORNE DIS, 7(1), 60-5. https://doi.org/10.1016/j.ttbdis.2015.08.005

Vancouver

Keller C, Krüger A, Schwarz NG, Rakotozandrindrainy R, Rakotondrainiarivelo JP, Razafindrabe T et al. High detection rate of Rickettsia africae in Amblyomma variegatum but low prevalence of anti-rickettsial antibodies in healthy pregnant women in Madagascar. TICKS TICK-BORNE DIS. 2016 Feb;7(1):60-5. https://doi.org/10.1016/j.ttbdis.2015.08.005

Bibtex

@article{a328d9f3a813429c990e5c72ee60d858,

title = "High detection rate of Rickettsia africae in Amblyomma variegatum but low prevalence of anti-rickettsial antibodies in healthy pregnant women in Madagascar",

abstract = "Tick-borne spotted fever group (SFG) rickettsioses are emerging infectious diseases in Sub-Saharan Africa. In Madagascar, the endemicity of tick-borne rickettsiae and their vectors has been incompletely studied. The first part of the present study was conducted in 2011 and 2012 to identify potential anthropophilic tick vectors for SFG rickettsiae on cattle from seven Malagasy regions, and to detect and characterize rickettsiae in these ticks. Amblyomma variegatum was the only anthropophilic tick species found on 262 cattle. Using a novel ompB-specific qPCR, screening for rickettsial DNA was performed on 111 A. variegatum ticks. Rickettsial DNA was detected in 96 of 111 ticks studied (86.5%). Rickettsia africae was identified as the only infecting rickettsia using phylogenetic analysis of ompA and ompB gene sequences and three variable intergenic spacers from 11 ticks. The second part of the study was a cross-sectional survey for antibodies against SFG rickettsiae in plasma samples taken from healthy, pregnant women at six locations in Madagascar, two at sea level and four between 450 and 1300m altitude. An indirect fluorescent antibody test with Rickettsia conorii as surrogate SFG rickettsial antigen was used. We found R. conorii-seropositives at all altitudes with prevalences between 0.5% and 3.1%. Our results suggest that A. variegatum ticks highly infected with R. africae are the most prevalent cattle-associated tick vectors for SFG rickettsiosis in Madagascar. Transmission of SFG rickettsiosis to humans occurs at different altitudes in Madagascar and should be considered as a relevant cause of febrile diseases.",

author = "Christian Keller and Andreas Kr{\"u}ger and Schwarz, {Norbert Georg} and Raphael Rakotozandrindrainy and Rakotondrainiarivelo, {Jean Philibert} and Tsiry Razafindrabe and Henri Derschum and Cornelia Silaghi and Daniela Pothmann and Alexandra Veit and Benedikt Hogan and J{\"u}rgen May and Mirko Girmann and Stefanie Kramme and Bernhard Fleischer and Sven Poppert",

year = "2016",

month = feb,

doi = "10.1016/j.ttbdis.2015.08.005",

language = "English",

volume = "7",

pages = "60--5",

journal = "TICKS TICK-BORNE DIS",

issn = "1877-959X",

publisher = "Elsevier GmbH",

number = "1",

}

RIS

TY - JOUR

T1 - High detection rate of Rickettsia africae in Amblyomma variegatum but low prevalence of anti-rickettsial antibodies in healthy pregnant women in Madagascar

AU - Keller, Christian

AU - Krüger, Andreas

AU - Schwarz, Norbert Georg

AU - Rakotozandrindrainy, Raphael

AU - Rakotondrainiarivelo, Jean Philibert

AU - Razafindrabe, Tsiry

AU - Derschum, Henri

AU - Silaghi, Cornelia

AU - Pothmann, Daniela

AU - Veit, Alexandra

AU - Hogan, Benedikt

AU - May, Jürgen

AU - Girmann, Mirko

AU - Kramme, Stefanie

AU - Fleischer, Bernhard

AU - Poppert, Sven

PY - 2016/2

Y1 - 2016/2

N2 - Tick-borne spotted fever group (SFG) rickettsioses are emerging infectious diseases in Sub-Saharan Africa. In Madagascar, the endemicity of tick-borne rickettsiae and their vectors has been incompletely studied. The first part of the present study was conducted in 2011 and 2012 to identify potential anthropophilic tick vectors for SFG rickettsiae on cattle from seven Malagasy regions, and to detect and characterize rickettsiae in these ticks. Amblyomma variegatum was the only anthropophilic tick species found on 262 cattle. Using a novel ompB-specific qPCR, screening for rickettsial DNA was performed on 111 A. variegatum ticks. Rickettsial DNA was detected in 96 of 111 ticks studied (86.5%). Rickettsia africae was identified as the only infecting rickettsia using phylogenetic analysis of ompA and ompB gene sequences and three variable intergenic spacers from 11 ticks. The second part of the study was a cross-sectional survey for antibodies against SFG rickettsiae in plasma samples taken from healthy, pregnant women at six locations in Madagascar, two at sea level and four between 450 and 1300m altitude. An indirect fluorescent antibody test with Rickettsia conorii as surrogate SFG rickettsial antigen was used. We found R. conorii-seropositives at all altitudes with prevalences between 0.5% and 3.1%. Our results suggest that A. variegatum ticks highly infected with R. africae are the most prevalent cattle-associated tick vectors for SFG rickettsiosis in Madagascar. Transmission of SFG rickettsiosis to humans occurs at different altitudes in Madagascar and should be considered as a relevant cause of febrile diseases.

AB - Tick-borne spotted fever group (SFG) rickettsioses are emerging infectious diseases in Sub-Saharan Africa. In Madagascar, the endemicity of tick-borne rickettsiae and their vectors has been incompletely studied. The first part of the present study was conducted in 2011 and 2012 to identify potential anthropophilic tick vectors for SFG rickettsiae on cattle from seven Malagasy regions, and to detect and characterize rickettsiae in these ticks. Amblyomma variegatum was the only anthropophilic tick species found on 262 cattle. Using a novel ompB-specific qPCR, screening for rickettsial DNA was performed on 111 A. variegatum ticks. Rickettsial DNA was detected in 96 of 111 ticks studied (86.5%). Rickettsia africae was identified as the only infecting rickettsia using phylogenetic analysis of ompA and ompB gene sequences and three variable intergenic spacers from 11 ticks. The second part of the study was a cross-sectional survey for antibodies against SFG rickettsiae in plasma samples taken from healthy, pregnant women at six locations in Madagascar, two at sea level and four between 450 and 1300m altitude. An indirect fluorescent antibody test with Rickettsia conorii as surrogate SFG rickettsial antigen was used. We found R. conorii-seropositives at all altitudes with prevalences between 0.5% and 3.1%. Our results suggest that A. variegatum ticks highly infected with R. africae are the most prevalent cattle-associated tick vectors for SFG rickettsiosis in Madagascar. Transmission of SFG rickettsiosis to humans occurs at different altitudes in Madagascar and should be considered as a relevant cause of febrile diseases.

U2 - 10.1016/j.ttbdis.2015.08.005

DO - 10.1016/j.ttbdis.2015.08.005

M3 - SCORING: Journal article

C2 - 26318262

VL - 7

SP - 60

EP - 65

JO - TICKS TICK-BORNE DIS

JF - TICKS TICK-BORNE DIS

SN - 1877-959X

IS - 1

ER -