Heterogeneity and chronology of PTEN deletion and ERG fusion in prostate cancer
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Heterogeneity and chronology of PTEN deletion and ERG fusion in prostate cancer. / Krohn, Antje; Freudenthaler, Fabian; Harasimowicz, Silvia; Kluth, Martina; Fuchs, Sarah; Burkhardt, Lia; Stahl, Phillip; C Tsourlakis, Maria; Bauer, Melanie; Tennstedt, Pierre; Graefen, Markus; Steurer, Stefan; Sirma, Hüseyin; Sauter, Guido; Schlomm, Thorsten; Simon, Ronald; Minner, Sarah.
in: MODERN PATHOL, Jahrgang 27, Nr. 12, 25.04.2014, S. 1612-1620.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Heterogeneity and chronology of PTEN deletion and ERG fusion in prostate cancer
AU - Krohn, Antje
AU - Freudenthaler, Fabian
AU - Harasimowicz, Silvia
AU - Kluth, Martina
AU - Fuchs, Sarah
AU - Burkhardt, Lia
AU - Stahl, Phillip
AU - C Tsourlakis, Maria
AU - Bauer, Melanie
AU - Tennstedt, Pierre
AU - Graefen, Markus
AU - Steurer, Stefan
AU - Sirma, Hüseyin
AU - Sauter, Guido
AU - Schlomm, Thorsten
AU - Simon, Ronald
AU - Minner, Sarah
PY - 2014/4/25
Y1 - 2014/4/25
N2 - TMPRSS2:ERG fusions, in combination with deletion of the phosphatase and tensin homolog (PTEN) tumor suppressor, have been suggested to cooperatively drive tumor progression in prostate cancer. We utilized a novel heterogeneity tissue microarray containing samples from 10 different tumor blocks of 189 prostatectomy specimens to study heterogeneity of genomic PTEN alterations in individual foci. PTEN alterations were found in 48/123 (39%) analyzable individual tumor foci, including 40 foci with deletions, 7 with deletion and rearrangement, and 1 focus with rearrangement only. PTEN was homogeneously aberrant in only 4 (8%) and heterogeneously in 44 (92%) of the foci. We found a specific sequence of molecular events from PTEN breakage followed by deletion of DNA sequences flanking the breakpoint, resulting in homozygous deletion. The observation that 16 of 19 foci with homogeneous ERG positivity had focal PTEN alterations but none of 10 foci with PTEN alterations had focal ERG positivity (P<0.0001) suggests that PTEN alterations typically develop subsequent to ERG fusions. We demonstrate a high level of intratumoral heterogeneity of PTEN alterations with deletions and rearrangements that challenges potential PTEN routine diagnosis testing in biopsies. The observation that PTEN alterations develop subsequent to ERG fusion strongly suggests that ERG expression may directly drive development of PTEN aberrations.Modern Pathology advance online publication, 25 April 2014; doi:10.1038/modpathol.2014.70.
AB - TMPRSS2:ERG fusions, in combination with deletion of the phosphatase and tensin homolog (PTEN) tumor suppressor, have been suggested to cooperatively drive tumor progression in prostate cancer. We utilized a novel heterogeneity tissue microarray containing samples from 10 different tumor blocks of 189 prostatectomy specimens to study heterogeneity of genomic PTEN alterations in individual foci. PTEN alterations were found in 48/123 (39%) analyzable individual tumor foci, including 40 foci with deletions, 7 with deletion and rearrangement, and 1 focus with rearrangement only. PTEN was homogeneously aberrant in only 4 (8%) and heterogeneously in 44 (92%) of the foci. We found a specific sequence of molecular events from PTEN breakage followed by deletion of DNA sequences flanking the breakpoint, resulting in homozygous deletion. The observation that 16 of 19 foci with homogeneous ERG positivity had focal PTEN alterations but none of 10 foci with PTEN alterations had focal ERG positivity (P<0.0001) suggests that PTEN alterations typically develop subsequent to ERG fusions. We demonstrate a high level of intratumoral heterogeneity of PTEN alterations with deletions and rearrangements that challenges potential PTEN routine diagnosis testing in biopsies. The observation that PTEN alterations develop subsequent to ERG fusion strongly suggests that ERG expression may directly drive development of PTEN aberrations.Modern Pathology advance online publication, 25 April 2014; doi:10.1038/modpathol.2014.70.
U2 - 10.1038/modpathol.2014.70
DO - 10.1038/modpathol.2014.70
M3 - SCORING: Journal article
C2 - 24762546
VL - 27
SP - 1612
EP - 1620
JO - MODERN PATHOL
JF - MODERN PATHOL
SN - 0893-3952
IS - 12
ER -