Herpes simplex virus type 1 entry into epithelial MDCKII cells: role of VASP activities.
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Herpes simplex virus type 1 entry into epithelial MDCKII cells: role of VASP activities. / Jaeger, Verena; Hoppe, Sven; Petermann, Philipp; Liebig, Timo; Jansen, Matthias K; Renné, Thomas; Knebel-Mörsdorf, Dagmar.
in: J GEN VIROL, Jahrgang 91, Nr. Pt 9, Pt 9, 2010, S. 2152-2157.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Herpes simplex virus type 1 entry into epithelial MDCKII cells: role of VASP activities.
AU - Jaeger, Verena
AU - Hoppe, Sven
AU - Petermann, Philipp
AU - Liebig, Timo
AU - Jansen, Matthias K
AU - Renné, Thomas
AU - Knebel-Mörsdorf, Dagmar
PY - 2010
Y1 - 2010
N2 - VASP is an actin-regulatory protein that links signalling to remodelling of the cytoskeleton. We investigated the role of VASP during entry of herpes simplex viruses into epithelial MDCKII cells. As VASP functions are regulated by phosphorylations, the phosphorylation pattern was determined upon infection. Phosphorylated VASP decreased temporarily at 15 and 30 min after infection. The impact of phosphorylated VASP was addressed by overexpression of phosphomimetic VASP mutants. Our results revealed that phosphorylated VASP slightly reduced the number of infected cells. Expression studies with deletion mutants further indicated minor effects of VASP on infection efficiency, whereas RNA interference studies demonstrated that reduced VASP expression did not suppress infection. We conclude that VASP activities alone may contribute to herpes simplex virus infection to only a minor extent.
AB - VASP is an actin-regulatory protein that links signalling to remodelling of the cytoskeleton. We investigated the role of VASP during entry of herpes simplex viruses into epithelial MDCKII cells. As VASP functions are regulated by phosphorylations, the phosphorylation pattern was determined upon infection. Phosphorylated VASP decreased temporarily at 15 and 30 min after infection. The impact of phosphorylated VASP was addressed by overexpression of phosphomimetic VASP mutants. Our results revealed that phosphorylated VASP slightly reduced the number of infected cells. Expression studies with deletion mutants further indicated minor effects of VASP on infection efficiency, whereas RNA interference studies demonstrated that reduced VASP expression did not suppress infection. We conclude that VASP activities alone may contribute to herpes simplex virus infection to only a minor extent.
KW - Animals
KW - Gene Deletion
KW - Dogs
KW - Mutation
KW - Gene Expression
KW - Amino Acid Substitution
KW - Phosphorylation
KW - Cell Line
KW - Transfection
KW - Mutagenesis, Site-Directed
KW - RNA Interference
KW - Recombinant Proteins/chemistry/genetics/metabolism
KW - Cell Adhesion Molecules/antagonists & inhibitors/chemistry/genetics/physiology
KW - Herpes Simplex/etiology/physiopathology/virology
KW - Herpesvirus 1, Human/pathogenicity/physiology
KW - Microfilament Proteins/antagonists & inhibitors/chemistry/genetics/physiology
KW - Phosphoproteins/antagonists & inhibitors/chemistry/genetics/physiology
KW - Serine/chemistry
KW - Virus Internalization
KW - Animals
KW - Gene Deletion
KW - Dogs
KW - Mutation
KW - Gene Expression
KW - Amino Acid Substitution
KW - Phosphorylation
KW - Cell Line
KW - Transfection
KW - Mutagenesis, Site-Directed
KW - RNA Interference
KW - Recombinant Proteins/chemistry/genetics/metabolism
KW - Cell Adhesion Molecules/antagonists & inhibitors/chemistry/genetics/physiology
KW - Herpes Simplex/etiology/physiopathology/virology
KW - Herpesvirus 1, Human/pathogenicity/physiology
KW - Microfilament Proteins/antagonists & inhibitors/chemistry/genetics/physiology
KW - Phosphoproteins/antagonists & inhibitors/chemistry/genetics/physiology
KW - Serine/chemistry
KW - Virus Internalization
M3 - SCORING: Journal article
VL - 91
SP - 2152
EP - 2157
JO - J GEN VIROL
JF - J GEN VIROL
SN - 0022-1317
IS - Pt 9
M1 - Pt 9
ER -
