Hemangioblasts and their progeny.
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Hemangioblasts and their progeny. / Gehling, Ursula.
in: METHOD ENZYMOL, Jahrgang 419, 2006, S. 179-193.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Hemangioblasts and their progeny.
AU - Gehling, Ursula
PY - 2006
Y1 - 2006
N2 - In the developing embryo, the hemangioblast, a mesodermal precursor, gives rise to hematopoietic and endothelial cells. Recent work has shown that during postnatal life, a subset of hematopoietic progenitor cells also displays this dual differentiation capacity and can function as endothelial progenitor cells that contribute to neovascularization. Thus, this subset might be useful for therapy of various hematopoietic and vascular diseases. Here, we describe a two-step culture system that results in the generation of endothelial and hematopoietic cells from adult progenitor cells with hemangioblastic potential. We have developed growth conditions that allow retroviral gene marking of the adult hemangioblast. This culture system is amenable for single-cell analyses at distinct stages of endothelial and hematopoietic differentiation from mobilized CD133+ progenitor cells.
AB - In the developing embryo, the hemangioblast, a mesodermal precursor, gives rise to hematopoietic and endothelial cells. Recent work has shown that during postnatal life, a subset of hematopoietic progenitor cells also displays this dual differentiation capacity and can function as endothelial progenitor cells that contribute to neovascularization. Thus, this subset might be useful for therapy of various hematopoietic and vascular diseases. Here, we describe a two-step culture system that results in the generation of endothelial and hematopoietic cells from adult progenitor cells with hemangioblastic potential. We have developed growth conditions that allow retroviral gene marking of the adult hemangioblast. This culture system is amenable for single-cell analyses at distinct stages of endothelial and hematopoietic differentiation from mobilized CD133+ progenitor cells.
M3 - SCORING: Zeitschriftenaufsatz
VL - 419
SP - 179
EP - 193
ER -