Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer

M V Dwek; H A Ross; A J Streets; S A Brooks; E Adam; A Titcomb; J V Woodside; U Schumacher; A J Leathem

Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer

Standard

Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer. / Dwek, M V; Ross, H A; Streets, A J; Brooks, S A; Adam, E; Titcomb, A; Woodside, J V; Schumacher, U; Leathem, A J.

in: INT J CANCER, Jahrgang 95, Nr. 2, 20.03.2001, S. 79-85.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Dwek, MV, Ross, HA, Streets, AJ, Brooks, SA, Adam, E, Titcomb, A, Woodside, JV, Schumacher, U & Leathem, AJ 2001, 'Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer', INT J CANCER, Jg. 95, Nr. 2, S. 79-85.

APA

Dwek, M. V., Ross, H. A., Streets, A. J., Brooks, S. A., Adam, E., Titcomb, A., Woodside, J. V., Schumacher, U., & Leathem, A. J. (2001). Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer. INT J CANCER, 95(2), 79-85.

Vancouver

Dwek MV, Ross HA, Streets AJ, Brooks SA, Adam E, Titcomb A et al. Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer. INT J CANCER. 2001 Mär 20;95(2):79-85.

Bibtex

@article{a399578f475149c3ad437af8ea330ef1,

title = "Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer",

abstract = "Predicting long-term outcome after breast-cancer diagnosis remains problematic, particularly for patients with clinically small, axillary lymph node- negative tumours. Evidence suggests that the lectin Helix pomatia agglutinin (HPA) identifies oligosaccharides associated with poor-prognosis cancer. Our aim was to identify oligosaccharides that bind HPA in aggressive breast cancers. Breast-cancer cell lines (MCF-7, BT-549 and BT-20) and a cell line from human milk (HBL-100), which showed a range of HPA-binding intensities, were used to extract HPA-binding glycoproteins. Oligosaccharides were released using anhydrous hydrazine and separated on a range of HPLC matrices. We investigated whether HPA-binding oligosaccharides from cell lines were present in human breast-cancer tissues, using 69 breast-cancer specimens from patients with between 5 and 10 years' follow-up. A monosialylated oligosaccharide was over-expressed in the cell line that bound HPA strongly. Further analysis by normal-phase HPLC showed that the 2-aminobenzamide-conjugated oligosaccharide had a hydrodynamic volume of 4.58 glucose units (HPAgly1). Increased expression of HPAgly1 was associated with HPA staining of breast-cancer specimens (Student's t-test p = 0.025). Analysis of oligosaccharide levels and disease-free survival after treatment for breast cancer indicated a shorter disease-free interval for patients with elevated levels of HPAgly1. This is the first time that histochemical lectin staining has been correlated with biochemical mapping of oligosaccharides. Using this approach, we have identified a monosialylated HPA lectin-binding oligosaccharide present in breast-cancer cells grown in vitro which is elevated in breast-cancer specimens that bind the lectin.",

keywords = "Adult, Breast Neoplasms, Carcinoma, Ductal, Breast, Cell Line, Chromatography, Affinity, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Disease-Free Survival, Female, Fluorescent Dyes, Humans, Lectins, Middle Aged, Oligosaccharides, Prognosis, Protein Binding, Retrospective Studies, Time Factors, Treatment Outcome, Tumor Cells, Cultured, ortho-Aminobenzoates",

author = "Dwek, {M V} and Ross, {H A} and Streets, {A J} and Brooks, {S A} and E Adam and A Titcomb and Woodside, {J V} and U Schumacher and Leathem, {A J}",

year = "2001",

month = mar,

day = "20",

language = "English",

volume = "95",

pages = "79--85",

journal = "INT J CANCER",

issn = "0020-7136",

publisher = "Wiley-Liss Inc.",

number = "2",

}

RIS

TY - JOUR

T1 - Helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer

AU - Dwek, M V

AU - Ross, H A

AU - Streets, A J

AU - Brooks, S A

AU - Adam, E

AU - Titcomb, A

AU - Woodside, J V

AU - Schumacher, U

AU - Leathem, A J

PY - 2001/3/20

Y1 - 2001/3/20

N2 - Predicting long-term outcome after breast-cancer diagnosis remains problematic, particularly for patients with clinically small, axillary lymph node- negative tumours. Evidence suggests that the lectin Helix pomatia agglutinin (HPA) identifies oligosaccharides associated with poor-prognosis cancer. Our aim was to identify oligosaccharides that bind HPA in aggressive breast cancers. Breast-cancer cell lines (MCF-7, BT-549 and BT-20) and a cell line from human milk (HBL-100), which showed a range of HPA-binding intensities, were used to extract HPA-binding glycoproteins. Oligosaccharides were released using anhydrous hydrazine and separated on a range of HPLC matrices. We investigated whether HPA-binding oligosaccharides from cell lines were present in human breast-cancer tissues, using 69 breast-cancer specimens from patients with between 5 and 10 years' follow-up. A monosialylated oligosaccharide was over-expressed in the cell line that bound HPA strongly. Further analysis by normal-phase HPLC showed that the 2-aminobenzamide-conjugated oligosaccharide had a hydrodynamic volume of 4.58 glucose units (HPAgly1). Increased expression of HPAgly1 was associated with HPA staining of breast-cancer specimens (Student's t-test p = 0.025). Analysis of oligosaccharide levels and disease-free survival after treatment for breast cancer indicated a shorter disease-free interval for patients with elevated levels of HPAgly1. This is the first time that histochemical lectin staining has been correlated with biochemical mapping of oligosaccharides. Using this approach, we have identified a monosialylated HPA lectin-binding oligosaccharide present in breast-cancer cells grown in vitro which is elevated in breast-cancer specimens that bind the lectin.

AB - Predicting long-term outcome after breast-cancer diagnosis remains problematic, particularly for patients with clinically small, axillary lymph node- negative tumours. Evidence suggests that the lectin Helix pomatia agglutinin (HPA) identifies oligosaccharides associated with poor-prognosis cancer. Our aim was to identify oligosaccharides that bind HPA in aggressive breast cancers. Breast-cancer cell lines (MCF-7, BT-549 and BT-20) and a cell line from human milk (HBL-100), which showed a range of HPA-binding intensities, were used to extract HPA-binding glycoproteins. Oligosaccharides were released using anhydrous hydrazine and separated on a range of HPLC matrices. We investigated whether HPA-binding oligosaccharides from cell lines were present in human breast-cancer tissues, using 69 breast-cancer specimens from patients with between 5 and 10 years' follow-up. A monosialylated oligosaccharide was over-expressed in the cell line that bound HPA strongly. Further analysis by normal-phase HPLC showed that the 2-aminobenzamide-conjugated oligosaccharide had a hydrodynamic volume of 4.58 glucose units (HPAgly1). Increased expression of HPAgly1 was associated with HPA staining of breast-cancer specimens (Student's t-test p = 0.025). Analysis of oligosaccharide levels and disease-free survival after treatment for breast cancer indicated a shorter disease-free interval for patients with elevated levels of HPAgly1. This is the first time that histochemical lectin staining has been correlated with biochemical mapping of oligosaccharides. Using this approach, we have identified a monosialylated HPA lectin-binding oligosaccharide present in breast-cancer cells grown in vitro which is elevated in breast-cancer specimens that bind the lectin.

KW - Adult

KW - Breast Neoplasms

KW - Carcinoma, Ductal, Breast

KW - Cell Line

KW - Chromatography, Affinity

KW - Chromatography, High Pressure Liquid

KW - Chromatography, Ion Exchange

KW - Disease-Free Survival

KW - Female

KW - Fluorescent Dyes

KW - Humans

KW - Lectins

KW - Middle Aged

KW - Oligosaccharides

KW - Prognosis

KW - Protein Binding

KW - Retrospective Studies

KW - Time Factors

KW - Treatment Outcome

KW - Tumor Cells, Cultured

KW - ortho-Aminobenzoates

M3 - SCORING: Journal article

C2 - 11241316

VL - 95

SP - 79

EP - 85

JO - INT J CANCER

JF - INT J CANCER

SN - 0020-7136

IS - 2

ER -