Heat stress reveals a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thaliana
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Heat stress reveals a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thaliana. / De Jaeger-Braet, Joke; Krause, Linda; Buchholz, Anika; Schnittger, Arp.
in: PLANT CELL, Jahrgang 34, Nr. 1, 20.01.2022, S. 433-454.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Heat stress reveals a specialized variant of the pachytene checkpoint in meiosis of Arabidopsis thaliana
AU - De Jaeger-Braet, Joke
AU - Krause, Linda
AU - Buchholz, Anika
AU - Schnittger, Arp
N1 - © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.
PY - 2022/1/20
Y1 - 2022/1/20
N2 - Plant growth and fertility strongly depend on environmental conditions such as temperature. Remarkably, temperature also influences meiotic recombination and thus, the current climate change will affect the genetic make-up of plants. To better understand the effects of temperature on meiosis, we followed male meiocytes in Arabidopsis thaliana by live cell imaging under three temperature regimes: at 21°C; at heat shock conditions of 30°C and 34°C; after an acclimatization phase of 1 week at 30°C. This work led to a cytological framework of meiotic progression at elevated temperature. We determined that an increase from 21°C to 30°C speeds up meiosis with specific phases being more amenable to heat than others. An acclimatization phase often moderated this effect. A sudden increase to 34°C promoted a faster progression of early prophase compared to 21°C. However, the phase in which cross-overs mature was prolonged at 34°C. Since mutants involved in the recombination pathway largely did not show the extension of this phase at 34°C, we conclude that the delay is recombination-dependent. Further analysis also revealed the involvement of the ATAXIA TELANGIECTASIA MUTATED kinase in this prolongation, indicating the existence of a pachytene checkpoint in plants, yet in a specialized form.
AB - Plant growth and fertility strongly depend on environmental conditions such as temperature. Remarkably, temperature also influences meiotic recombination and thus, the current climate change will affect the genetic make-up of plants. To better understand the effects of temperature on meiosis, we followed male meiocytes in Arabidopsis thaliana by live cell imaging under three temperature regimes: at 21°C; at heat shock conditions of 30°C and 34°C; after an acclimatization phase of 1 week at 30°C. This work led to a cytological framework of meiotic progression at elevated temperature. We determined that an increase from 21°C to 30°C speeds up meiosis with specific phases being more amenable to heat than others. An acclimatization phase often moderated this effect. A sudden increase to 34°C promoted a faster progression of early prophase compared to 21°C. However, the phase in which cross-overs mature was prolonged at 34°C. Since mutants involved in the recombination pathway largely did not show the extension of this phase at 34°C, we conclude that the delay is recombination-dependent. Further analysis also revealed the involvement of the ATAXIA TELANGIECTASIA MUTATED kinase in this prolongation, indicating the existence of a pachytene checkpoint in plants, yet in a specialized form.
U2 - 10.1093/plcell/koab257
DO - 10.1093/plcell/koab257
M3 - SCORING: Journal article
C2 - 34718750
VL - 34
SP - 433
EP - 454
JO - PLANT CELL
JF - PLANT CELL
SN - 1040-4651
IS - 1
ER -