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Heart-targeted adeno-associated viral vectors selected by in vivo biopanning of a random viral display peptide library

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Heart-targeted adeno-associated viral vectors selected by in vivo biopanning of a random viral display peptide library. / Ying, Y; Müller, O J; Goehringer, C; Leuchs, B; Trepel, M; Katus, H A; Kleinschmidt, J A.

in: GENE THER, Jahrgang 17, Nr. 8, 08.2010, S. 980-90.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Ying, Y, Müller, OJ, Goehringer, C, Leuchs, B, Trepel, M, Katus, HA & Kleinschmidt, JA 2010, 'Heart-targeted adeno-associated viral vectors selected by in vivo biopanning of a random viral display peptide library', GENE THER, Jg. 17, Nr. 8, S. 980-90. https://doi.org/10.1038/gt.2010.44

APA

Ying, Y., Müller, O. J., Goehringer, C., Leuchs, B., Trepel, M., Katus, H. A., & Kleinschmidt, J. A. (2010). Heart-targeted adeno-associated viral vectors selected by in vivo biopanning of a random viral display peptide library. GENE THER, 17(8), 980-90. https://doi.org/10.1038/gt.2010.44

Vancouver

Ying Y, Müller OJ, Goehringer C, Leuchs B, Trepel M, Katus HA et al. Heart-targeted adeno-associated viral vectors selected by in vivo biopanning of a random viral display peptide library. GENE THER. 2010 Aug;17(8):980-90. https://doi.org/10.1038/gt.2010.44

Bibtex

@article{bbcc44565f494cf6b673c2ac239d0db5,
title = "Heart-targeted adeno-associated viral vectors selected by in vivo biopanning of a random viral display peptide library",
abstract = "Selection of targeted vectors from virus display peptide libraries is a versatile and efficient approach to improve vector specificity and efficiency. This strategy has been used to target various cell types in vitro. Here, we report the screening of an adeno-associated virus type 2 (AAV2) display peptide library in vivo to select vectors specifically homing to heart tissue after systemic application in mice. Selected library clones indicated superior specificity of gene transfer compared with wild-type AAV2, AAV9 and a heparin binding-deficient AAV2 mutant. Such targeted vectors were able to reconstitute expression of delta-sarcoglycan in the heart of adult delta-sarcoglycan knockout mice after systemic gene transfer in vivo, attesting to the therapeutic potential of this approach.",
keywords = "Animals, Cell Line, Dependovirus, Gene Transfer Techniques, Genetic Therapy, Genetic Vectors, Mice, Myocardium, Myocytes, Cardiac, Peptide Library, Rats, Sarcoglycans, Transduction, Genetic, Journal Article, Research Support, Non-U.S. Gov't",
author = "Y Ying and M{\"u}ller, {O J} and C Goehringer and B Leuchs and M Trepel and Katus, {H A} and Kleinschmidt, {J A}",
year = "2010",
month = aug,
doi = "10.1038/gt.2010.44",
language = "English",
volume = "17",
pages = "980--90",
journal = "GENE THER",
issn = "0969-7128",
publisher = "NATURE PUBLISHING GROUP",
number = "8",

}

RIS

TY - JOUR

T1 - Heart-targeted adeno-associated viral vectors selected by in vivo biopanning of a random viral display peptide library

AU - Ying, Y

AU - Müller, O J

AU - Goehringer, C

AU - Leuchs, B

AU - Trepel, M

AU - Katus, H A

AU - Kleinschmidt, J A

PY - 2010/8

Y1 - 2010/8

N2 - Selection of targeted vectors from virus display peptide libraries is a versatile and efficient approach to improve vector specificity and efficiency. This strategy has been used to target various cell types in vitro. Here, we report the screening of an adeno-associated virus type 2 (AAV2) display peptide library in vivo to select vectors specifically homing to heart tissue after systemic application in mice. Selected library clones indicated superior specificity of gene transfer compared with wild-type AAV2, AAV9 and a heparin binding-deficient AAV2 mutant. Such targeted vectors were able to reconstitute expression of delta-sarcoglycan in the heart of adult delta-sarcoglycan knockout mice after systemic gene transfer in vivo, attesting to the therapeutic potential of this approach.

AB - Selection of targeted vectors from virus display peptide libraries is a versatile and efficient approach to improve vector specificity and efficiency. This strategy has been used to target various cell types in vitro. Here, we report the screening of an adeno-associated virus type 2 (AAV2) display peptide library in vivo to select vectors specifically homing to heart tissue after systemic application in mice. Selected library clones indicated superior specificity of gene transfer compared with wild-type AAV2, AAV9 and a heparin binding-deficient AAV2 mutant. Such targeted vectors were able to reconstitute expression of delta-sarcoglycan in the heart of adult delta-sarcoglycan knockout mice after systemic gene transfer in vivo, attesting to the therapeutic potential of this approach.

KW - Animals

KW - Cell Line

KW - Dependovirus

KW - Gene Transfer Techniques

KW - Genetic Therapy

KW - Genetic Vectors

KW - Mice

KW - Myocardium

KW - Myocytes, Cardiac

KW - Peptide Library

KW - Rats

KW - Sarcoglycans

KW - Transduction, Genetic

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1038/gt.2010.44

DO - 10.1038/gt.2010.44

M3 - SCORING: Journal article

C2 - 20393510

VL - 17

SP - 980

EP - 990

JO - GENE THER

JF - GENE THER

SN - 0969-7128

IS - 8

ER -