Haematopoietic cell transplantation outcomes are linked to intestinal mycobiota dynamics and an expansion of Candida parapsilosis complex species
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Haematopoietic cell transplantation outcomes are linked to intestinal mycobiota dynamics and an expansion of Candida parapsilosis complex species. / Rolling, Thierry; Zhai, Bing; Gjonbalaj, Mergim; Tosini, Nicholas; Yasuma-Mitobe, Keiko; Fontana, Emily; Amoretti, Luigi A; Wright, Roberta J; Ponce, Doris M; Perales, Miguel A; Xavier, Joao B; van den Brink, Marcel R M; Markey, Kate A; Peled, Jonathan U; Taur, Ying; Hohl, Tobias M.
in: NAT MICROBIOL, Jahrgang 6, Nr. 12, 12.2021, S. 1505-1515.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Haematopoietic cell transplantation outcomes are linked to intestinal mycobiota dynamics and an expansion of Candida parapsilosis complex species
AU - Rolling, Thierry
AU - Zhai, Bing
AU - Gjonbalaj, Mergim
AU - Tosini, Nicholas
AU - Yasuma-Mitobe, Keiko
AU - Fontana, Emily
AU - Amoretti, Luigi A
AU - Wright, Roberta J
AU - Ponce, Doris M
AU - Perales, Miguel A
AU - Xavier, Joao B
AU - van den Brink, Marcel R M
AU - Markey, Kate A
AU - Peled, Jonathan U
AU - Taur, Ying
AU - Hohl, Tobias M
N1 - © 2021. The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2021/12
Y1 - 2021/12
N2 - Allogeneic haematopoietic cell transplantation (allo-HCT) induces profound shifts in the intestinal bacterial microbiota. The dynamics of intestinal fungi and their impact on clinical outcomes during allo-HCT are not fully understood. Here we combined parallel high-throughput fungal ITS1 amplicon sequencing, bacterial 16S amplicon sequencing and fungal cultures of 1,279 faecal samples from a cohort of 156 patients undergoing allo-HCT to reveal potential trans-kingdom dynamics and their association with patient outcomes. We saw that the overall density and the biodiversity of intestinal fungi were stable during allo-HCT but the species composition changed drastically from day to day. We identified a subset of patients with fungal dysbiosis defined by culture positivity (n = 53) and stable expansion of Candida parapsilosis complex species (n = 19). They presented with distinct trans-kingdom microbiota profiles, characterized by a decreased intestinal bacterial biomass. These patients had worse overall survival and higher transplant-related mortality independent of candidaemia. This expands our understanding of the clinical significance of the mycobiota and suggests that targeting fungal dysbiosis may help to improve long-term patient survival.
AB - Allogeneic haematopoietic cell transplantation (allo-HCT) induces profound shifts in the intestinal bacterial microbiota. The dynamics of intestinal fungi and their impact on clinical outcomes during allo-HCT are not fully understood. Here we combined parallel high-throughput fungal ITS1 amplicon sequencing, bacterial 16S amplicon sequencing and fungal cultures of 1,279 faecal samples from a cohort of 156 patients undergoing allo-HCT to reveal potential trans-kingdom dynamics and their association with patient outcomes. We saw that the overall density and the biodiversity of intestinal fungi were stable during allo-HCT but the species composition changed drastically from day to day. We identified a subset of patients with fungal dysbiosis defined by culture positivity (n = 53) and stable expansion of Candida parapsilosis complex species (n = 19). They presented with distinct trans-kingdom microbiota profiles, characterized by a decreased intestinal bacterial biomass. These patients had worse overall survival and higher transplant-related mortality independent of candidaemia. This expands our understanding of the clinical significance of the mycobiota and suggests that targeting fungal dysbiosis may help to improve long-term patient survival.
U2 - 10.1038/s41564-021-00989-7
DO - 10.1038/s41564-021-00989-7
M3 - SCORING: Journal article
C2 - 34764444
VL - 6
SP - 1505
EP - 1515
JO - NAT MICROBIOL
JF - NAT MICROBIOL
SN - 2058-5276
IS - 12
ER -