H3K64 trimethylation marks heterochromatin and is dynamically remodeled during developmental reprogramming
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H3K64 trimethylation marks heterochromatin and is dynamically remodeled during developmental reprogramming. / Daujat, Sylvain; Weiss, Thomas; Mohn, Fabio; Lange, Ulrike C; Ziegler-Birling, Céline; Zeissler, Ulrike; Lappe, Michael; Schübeler, Dirk; Torres-Padilla, Maria-Elena; Schneider, Robert.
in: NAT STRUCT MOL BIOL, Jahrgang 16, Nr. 7, 01.07.2009, S. 777-81.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - H3K64 trimethylation marks heterochromatin and is dynamically remodeled during developmental reprogramming
AU - Daujat, Sylvain
AU - Weiss, Thomas
AU - Mohn, Fabio
AU - Lange, Ulrike C
AU - Ziegler-Birling, Céline
AU - Zeissler, Ulrike
AU - Lappe, Michael
AU - Schübeler, Dirk
AU - Torres-Padilla, Maria-Elena
AU - Schneider, Robert
PY - 2009/7/1
Y1 - 2009/7/1
N2 - Histone modifications are central to the regulation of all DNA-dependent processes. Lys64 of histone H3 (H3K64) lies within the globular domain at a structurally important position. We identify trimethylation of H3K64 (H3K64me3) as a modification that is enriched at pericentric heterochromatin and associated with repeat sequences and transcriptionally inactive genomic regions. We show that this new mark is dynamic during the two main epigenetic reprogramming events in mammals. In primordial germ cells, H3K64me3 is present at the time of specification, but it disappears transiently during reprogramming. In early mouse embryos, it is inherited exclusively maternally; subsequently, the modification is rapidly removed, suggesting an important role for H3K64me3 turnover in development. Taken together, our findings establish H3K64me3 as a previously uncharacterized histone modification that is preferentially localized to repressive chromatin. We hypothesize that H3K64me3 helps to 'secure' nucleosomes, and perhaps the surrounding chromatin, in an appropriately repressed state during development.
AB - Histone modifications are central to the regulation of all DNA-dependent processes. Lys64 of histone H3 (H3K64) lies within the globular domain at a structurally important position. We identify trimethylation of H3K64 (H3K64me3) as a modification that is enriched at pericentric heterochromatin and associated with repeat sequences and transcriptionally inactive genomic regions. We show that this new mark is dynamic during the two main epigenetic reprogramming events in mammals. In primordial germ cells, H3K64me3 is present at the time of specification, but it disappears transiently during reprogramming. In early mouse embryos, it is inherited exclusively maternally; subsequently, the modification is rapidly removed, suggesting an important role for H3K64me3 turnover in development. Taken together, our findings establish H3K64me3 as a previously uncharacterized histone modification that is preferentially localized to repressive chromatin. We hypothesize that H3K64me3 helps to 'secure' nucleosomes, and perhaps the surrounding chromatin, in an appropriately repressed state during development.
KW - Histone
KW - Cell Line
KW - chromatin
KW - DNA Methylation
KW - Embryo, Mammalian
KW - Epigenetics
KW - Heterochromatin
KW - Histones
KW - Lysine
KW - Methyltransferases
KW - Mice
KW - Molecular Sequence Data
KW - Protein Conformation
U2 - 10.1038/nsmb.1629
DO - 10.1038/nsmb.1629
M3 - SCORING: Journal article
C2 - 19561610
VL - 16
SP - 777
EP - 781
JO - NAT STRUCT MOL BIOL
JF - NAT STRUCT MOL BIOL
SN - 1545-9993
IS - 7
ER -
