Growth rate related concentration changes of the starvation response regulators sigmaS and ppGpp in glucose-limited fed-batch and continuous cultures of Escherichia coli.
Standard
Growth rate related concentration changes of the starvation response regulators sigmaS and ppGpp in glucose-limited fed-batch and continuous cultures of Escherichia coli. / Teich, A; Meyer, S; Lin, Hongying; Andersson, L; Enfors, S; Neubauer, P.
in: BIOTECHNOL PROGR, Jahrgang 15, Nr. 1, 1, 1999, S. 123-129.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Growth rate related concentration changes of the starvation response regulators sigmaS and ppGpp in glucose-limited fed-batch and continuous cultures of Escherichia coli.
AU - Teich, A
AU - Meyer, S
AU - Lin, Hongying
AU - Andersson, L
AU - Enfors, S
AU - Neubauer, P
PY - 1999
Y1 - 1999
N2 - The entry of Escherichia coli into glucose starvation is connected to the induction of both the stringent response and the general stress response. The concentration of the corresponding response regulators ppGpp and sigmaS (RpoS, KatF) was investigated during glucose-limited growth at different growth rates and with different cultivation strategies, including fed-batch fermentation, chemostat, and a continuous-cultivation technique with computer-controlled decrease of the feeding rate (A-stat). This study confirms the model of the in vivo effect of ppGpp on sigmaS by the direct measurement of ppGpp and sigmaS levels. The response of both regulators was clearly shown in situations where a nutrient shift occurs. A correlation in the concentration of both regulators was also shown under steady-state conditions. However, during transients with slow continuous changes of the nutrient availability, this concerted reaction of ppGpp and sigmaS is less apparent, indicating the specific importance of these regulators for the adaptation of the cells to fast changes of environmental parameters.
AB - The entry of Escherichia coli into glucose starvation is connected to the induction of both the stringent response and the general stress response. The concentration of the corresponding response regulators ppGpp and sigmaS (RpoS, KatF) was investigated during glucose-limited growth at different growth rates and with different cultivation strategies, including fed-batch fermentation, chemostat, and a continuous-cultivation technique with computer-controlled decrease of the feeding rate (A-stat). This study confirms the model of the in vivo effect of ppGpp on sigmaS by the direct measurement of ppGpp and sigmaS levels. The response of both regulators was clearly shown in situations where a nutrient shift occurs. A correlation in the concentration of both regulators was also shown under steady-state conditions. However, during transients with slow continuous changes of the nutrient availability, this concerted reaction of ppGpp and sigmaS is less apparent, indicating the specific importance of these regulators for the adaptation of the cells to fast changes of environmental parameters.
M3 - SCORING: Zeitschriftenaufsatz
VL - 15
SP - 123
EP - 129
JO - BIOTECHNOL PROGR
JF - BIOTECHNOL PROGR
SN - 8756-7938
IS - 1
M1 - 1
ER -
