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Growth behavior, matrix production, and gene expression of human osteoblasts in defined cylindrical titanium channels.

Standard

Growth behavior, matrix production, and gene expression of human osteoblasts in defined cylindrical titanium channels. / Frosch, Karl-Heinz; Barvencik, Florian; Viereck, Volker; Lohmann, Christoph; Dresing, Klaus; Breme, Jürgen; Brunner, Edgar; Stürmer, Klaus Michael.

in: J BIOMED MATER RES A, Jahrgang 68, Nr. 2, 2, 2004, S. 325-334.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung

Harvard

Frosch, K-H, Barvencik, F, Viereck, V, Lohmann, C, Dresing, K, Breme, J, Brunner, E & Stürmer, KM 2004, 'Growth behavior, matrix production, and gene expression of human osteoblasts in defined cylindrical titanium channels.', J BIOMED MATER RES A, Jg. 68, Nr. 2, 2, S. 325-334. <http://www.ncbi.nlm.nih.gov/pubmed/14704974?dopt=Citation>

APA

Frosch, K-H., Barvencik, F., Viereck, V., Lohmann, C., Dresing, K., Breme, J., Brunner, E., & Stürmer, K. M. (2004). Growth behavior, matrix production, and gene expression of human osteoblasts in defined cylindrical titanium channels. J BIOMED MATER RES A, 68(2), 325-334. [2]. http://www.ncbi.nlm.nih.gov/pubmed/14704974?dopt=Citation

Vancouver

Frosch K-H, Barvencik F, Viereck V, Lohmann C, Dresing K, Breme J et al. Growth behavior, matrix production, and gene expression of human osteoblasts in defined cylindrical titanium channels. J BIOMED MATER RES A. 2004;68(2):325-334. 2.

Bibtex

@article{ff45784135cb4ec8a99142b84003d800,
title = "Growth behavior, matrix production, and gene expression of human osteoblasts in defined cylindrical titanium channels.",
abstract = "The purpose of the current study was to investigate the effect of different diameters of cylindrical titanium channels on human osteoblasts. Titanium samples having continuous drill channels with diameters of 300, 400, 500, 600, and 1000 microm were put into osteoblast cell cultures that were isolated from 12 adult human trauma patients. Cell migration into the drill channels was investigated by transmitted-light microscopy. The DNA content in the drill channels was measured photometrically, collagen type I production was analyzed by enzyme-linked immunosorbent assay (ELISA) and osteocalcin gene expression by reverse transcriptase-polymerase chain reaction (RT-PCR). Formation of mineralized tissue was assessed by microradiographs of histological sections. Within 20 days, cells grew an average of 838 microm (+/-128 microm) into the drill channels with a diameter of 600 microm and were significantly faster (p <0.05) than in all other channels. Cells produced significantly more osteocalcin messenger RNA (mRNA) in 600-microm channels (p <0.05) than they did in 1000-microm channels and demonstrated the highest osteogenic differentiation. The channel diameter did not influence collagen type I production. The highest cell density was found in 300-microm channels (p <0.05). The DNA content of the channels linearly decreased with increasing channel diameters. After 40 days of culture, the proportion of mineralized tissue at the mouth section amounted to 6% in 300-microm channels and to 9-11% in 400-600-microm channels. In 1000-microm channels, only traces of mineralization were detected. Our data suggest that the diameter of cylindrical titanium channels has a significant effect on migration, gene expression, and mineralization of human osteoblasts.",
author = "Karl-Heinz Frosch and Florian Barvencik and Volker Viereck and Christoph Lohmann and Klaus Dresing and J{\"u}rgen Breme and Edgar Brunner and St{\"u}rmer, {Klaus Michael}",
year = "2004",
language = "Deutsch",
volume = "68",
pages = "325--334",
journal = "J BIOMED MATER RES A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Growth behavior, matrix production, and gene expression of human osteoblasts in defined cylindrical titanium channels.

AU - Frosch, Karl-Heinz

AU - Barvencik, Florian

AU - Viereck, Volker

AU - Lohmann, Christoph

AU - Dresing, Klaus

AU - Breme, Jürgen

AU - Brunner, Edgar

AU - Stürmer, Klaus Michael

PY - 2004

Y1 - 2004

N2 - The purpose of the current study was to investigate the effect of different diameters of cylindrical titanium channels on human osteoblasts. Titanium samples having continuous drill channels with diameters of 300, 400, 500, 600, and 1000 microm were put into osteoblast cell cultures that were isolated from 12 adult human trauma patients. Cell migration into the drill channels was investigated by transmitted-light microscopy. The DNA content in the drill channels was measured photometrically, collagen type I production was analyzed by enzyme-linked immunosorbent assay (ELISA) and osteocalcin gene expression by reverse transcriptase-polymerase chain reaction (RT-PCR). Formation of mineralized tissue was assessed by microradiographs of histological sections. Within 20 days, cells grew an average of 838 microm (+/-128 microm) into the drill channels with a diameter of 600 microm and were significantly faster (p <0.05) than in all other channels. Cells produced significantly more osteocalcin messenger RNA (mRNA) in 600-microm channels (p <0.05) than they did in 1000-microm channels and demonstrated the highest osteogenic differentiation. The channel diameter did not influence collagen type I production. The highest cell density was found in 300-microm channels (p <0.05). The DNA content of the channels linearly decreased with increasing channel diameters. After 40 days of culture, the proportion of mineralized tissue at the mouth section amounted to 6% in 300-microm channels and to 9-11% in 400-600-microm channels. In 1000-microm channels, only traces of mineralization were detected. Our data suggest that the diameter of cylindrical titanium channels has a significant effect on migration, gene expression, and mineralization of human osteoblasts.

AB - The purpose of the current study was to investigate the effect of different diameters of cylindrical titanium channels on human osteoblasts. Titanium samples having continuous drill channels with diameters of 300, 400, 500, 600, and 1000 microm were put into osteoblast cell cultures that were isolated from 12 adult human trauma patients. Cell migration into the drill channels was investigated by transmitted-light microscopy. The DNA content in the drill channels was measured photometrically, collagen type I production was analyzed by enzyme-linked immunosorbent assay (ELISA) and osteocalcin gene expression by reverse transcriptase-polymerase chain reaction (RT-PCR). Formation of mineralized tissue was assessed by microradiographs of histological sections. Within 20 days, cells grew an average of 838 microm (+/-128 microm) into the drill channels with a diameter of 600 microm and were significantly faster (p <0.05) than in all other channels. Cells produced significantly more osteocalcin messenger RNA (mRNA) in 600-microm channels (p <0.05) than they did in 1000-microm channels and demonstrated the highest osteogenic differentiation. The channel diameter did not influence collagen type I production. The highest cell density was found in 300-microm channels (p <0.05). The DNA content of the channels linearly decreased with increasing channel diameters. After 40 days of culture, the proportion of mineralized tissue at the mouth section amounted to 6% in 300-microm channels and to 9-11% in 400-600-microm channels. In 1000-microm channels, only traces of mineralization were detected. Our data suggest that the diameter of cylindrical titanium channels has a significant effect on migration, gene expression, and mineralization of human osteoblasts.

M3 - SCORING: Zeitschriftenaufsatz

VL - 68

SP - 325

EP - 334

JO - J BIOMED MATER RES A

JF - J BIOMED MATER RES A

SN - 1549-3296

IS - 2

M1 - 2

ER -