Granulocyte colony-stimulating factor increases hepatic sinusoidal perfusion during liver regeneration in mice
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Granulocyte colony-stimulating factor increases hepatic sinusoidal perfusion during liver regeneration in mice. / Sidler, Daniel; Studer, Peter; Küpper, Sebastian; Gloor, Beat; Candinas, Daniel; Haier, Jörg; Inderbitzin, Daniel.
in: J INVEST SURG, Jahrgang 21, Nr. 2, 15.03.2008, S. 57-64.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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TY - JOUR
T1 - Granulocyte colony-stimulating factor increases hepatic sinusoidal perfusion during liver regeneration in mice
AU - Sidler, Daniel
AU - Studer, Peter
AU - Küpper, Sebastian
AU - Gloor, Beat
AU - Candinas, Daniel
AU - Haier, Jörg
AU - Inderbitzin, Daniel
PY - 2008/3/15
Y1 - 2008/3/15
N2 - Conditioning with granulocyte colony-stimulating factor (G-CSF) promotes liver regeneration in an experimental small-for-size liver remnant mouse model. The mechanisms involved in this extraordinary G-CSF effect are unknown. The aim of this study was to investigate the influence of G-CSF on the hepatic microvasculature in the regenerating liver. The hepatic sinusoidal microvasculature and microarchitecture of the regenerating liver were evaluated by intravital microscopy in mice. Three experimental groups were compared: (1) unoperated unconditioned animals (control; n = 5), (2) animals conditioned with G-CSF 48 h after 60% partial hepatectomy (G-CSF-PH; n = 6), and (3) animals sham conditioned 48 h after 60% PH (sham-PH; n = 6). PH led to hepatocyte hypertrophy and increased hepatic sinusoidal velocity in the sham-PH and G-CSF-PH groups. Increased sinusoidal diameter and increased hepatic blood flow were observed in the G-CSF-PH group compared to the sham-PH and control groups. Furthermore, there was a strong positive correlation between spleen weight and hepatic sinusoidal diameter in the G-CSF-PH group. The increased hepatic blood flow could explain the observed benefit of G-CSF conditioning during liver regeneration. These results elucidate an unexplored aspect of pharmacological modulation of liver regeneration and motivate further experiments.
AB - Conditioning with granulocyte colony-stimulating factor (G-CSF) promotes liver regeneration in an experimental small-for-size liver remnant mouse model. The mechanisms involved in this extraordinary G-CSF effect are unknown. The aim of this study was to investigate the influence of G-CSF on the hepatic microvasculature in the regenerating liver. The hepatic sinusoidal microvasculature and microarchitecture of the regenerating liver were evaluated by intravital microscopy in mice. Three experimental groups were compared: (1) unoperated unconditioned animals (control; n = 5), (2) animals conditioned with G-CSF 48 h after 60% partial hepatectomy (G-CSF-PH; n = 6), and (3) animals sham conditioned 48 h after 60% PH (sham-PH; n = 6). PH led to hepatocyte hypertrophy and increased hepatic sinusoidal velocity in the sham-PH and G-CSF-PH groups. Increased sinusoidal diameter and increased hepatic blood flow were observed in the G-CSF-PH group compared to the sham-PH and control groups. Furthermore, there was a strong positive correlation between spleen weight and hepatic sinusoidal diameter in the G-CSF-PH group. The increased hepatic blood flow could explain the observed benefit of G-CSF conditioning during liver regeneration. These results elucidate an unexplored aspect of pharmacological modulation of liver regeneration and motivate further experiments.
KW - Animals
KW - Granulocyte Colony-Stimulating Factor
KW - Hepatectomy
KW - Immunohistochemistry
KW - Ki-67 Antigen
KW - Liver
KW - Liver Circulation
KW - Liver Regeneration
KW - Male
KW - Mice
KW - Mice, Inbred BALB C
KW - Microcirculation
KW - Organ Size
U2 - 10.1080/08941930701883632
DO - 10.1080/08941930701883632
M3 - SCORING: Journal article
C2 - 18340621
VL - 21
SP - 57
EP - 64
JO - J INVEST SURG
JF - J INVEST SURG
SN - 0894-1939
IS - 2
ER -
