Germline ablation of dermatan-4O-sulfotransferase1 reduces regeneration after mouse spinal cord injury
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Germline ablation of dermatan-4O-sulfotransferase1 reduces regeneration after mouse spinal cord injury. / Rost, S; Akyüz, N; Martinovic, Tamara; Huckhagel, T; Jakovcevski, I; Schachner, M.
in: NEUROSCIENCE, Jahrgang 312, 14.11.2015, S. 74-85.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
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T1 - Germline ablation of dermatan-4O-sulfotransferase1 reduces regeneration after mouse spinal cord injury
AU - Rost, S
AU - Akyüz, N
AU - Martinovic, Tamara
AU - Huckhagel, T
AU - Jakovcevski, I
AU - Schachner, M
N1 - Copyright © 2015 IBRO. Published by Elsevier Ltd. All rights reserved.
PY - 2015/11/14
Y1 - 2015/11/14
N2 - Chondroitin/dermatan sulfate proteoglycans (CSPGs/DSPGs) are major components of the extracellular matrix. Their expression is generally upregulated after injuries to the adult mammalian central nervous system, which is known for its low ability to restore function after injury. Several studies support the view that CSPGs inhibit regeneration after injury, whereas the functions of DSPGs in injury paradigms are less certain. To characterize the functions of DSPGs in the presence of CSPGs, we studied young adult dermatan-4O-sulfotransferase1-deficient (Chst14(-/-)) mice, which express chondroitin sulfates (CSs), but not dermatan sulfates (DSs), to characterize the functional outcome after severe compression injury of the spinal cord. In comparison to their wild-type (Chst14(+/+)) littermates, regeneration was reduced in Chst14(-/-) mice. No differences between genotypes were seen in the size of spinal cords, numbers of microglia and astrocytes neither in intact nor injured spinal cords after injury. Monoaminergic innervation and re-innervation of the spinal cord caudal to the lesion site as well as expression levels of glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) were similar in both genotypes, independent of whether they were injured and examined 6weeks after injury or not injured. These results suggest that, in contrast to CSPGs, DSPGs, being the products of Chst14 enzymatic activity, promote regeneration after injury of the adult mouse central nervous system.
AB - Chondroitin/dermatan sulfate proteoglycans (CSPGs/DSPGs) are major components of the extracellular matrix. Their expression is generally upregulated after injuries to the adult mammalian central nervous system, which is known for its low ability to restore function after injury. Several studies support the view that CSPGs inhibit regeneration after injury, whereas the functions of DSPGs in injury paradigms are less certain. To characterize the functions of DSPGs in the presence of CSPGs, we studied young adult dermatan-4O-sulfotransferase1-deficient (Chst14(-/-)) mice, which express chondroitin sulfates (CSs), but not dermatan sulfates (DSs), to characterize the functional outcome after severe compression injury of the spinal cord. In comparison to their wild-type (Chst14(+/+)) littermates, regeneration was reduced in Chst14(-/-) mice. No differences between genotypes were seen in the size of spinal cords, numbers of microglia and astrocytes neither in intact nor injured spinal cords after injury. Monoaminergic innervation and re-innervation of the spinal cord caudal to the lesion site as well as expression levels of glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) were similar in both genotypes, independent of whether they were injured and examined 6weeks after injury or not injured. These results suggest that, in contrast to CSPGs, DSPGs, being the products of Chst14 enzymatic activity, promote regeneration after injury of the adult mouse central nervous system.
U2 - 10.1016/j.neuroscience.2015.11.013
DO - 10.1016/j.neuroscience.2015.11.013
M3 - SCORING: Journal article
C2 - 26586562
VL - 312
SP - 74
EP - 85
JO - NEUROSCIENCE
JF - NEUROSCIENCE
SN - 0306-4522
ER -
