Genomic signature of BRCA1 deficiency in sporadic basal-like breast tumors.
Standard
Genomic signature of BRCA1 deficiency in sporadic basal-like breast tumors. / Joosse, Simon; Brandwijk, Kim I M; Mulder, Lennart; Wesseling, Jelle; Hannemann, Juliane; Nederlof, Petra M.
in: GENE CHROMOSOME CANC, Jahrgang 50, Nr. 2, 2, 2011, S. 71-81.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Genomic signature of BRCA1 deficiency in sporadic basal-like breast tumors.
AU - Joosse, Simon
AU - Brandwijk, Kim I M
AU - Mulder, Lennart
AU - Wesseling, Jelle
AU - Hannemann, Juliane
AU - Nederlof, Petra M
PY - 2011
Y1 - 2011
N2 - About 10-20% of all breast carcinomas show a basal-like phenotype, while ? 90% of breast tumors from BRCA1-mutation carriers are of this subtype. There is growing evidence that BRCA1-mutated tumors are not just a specific subset of the basal-like tumors, but that (the majority of) basal-like tumors show a dysfunctional BRCA1 pathway. This has major treatment implications, because emerging regimens specifically targeting DNA repair mechanisms would then be most effective against these tumors. To further understand the involvement of BRCA1 deficiency in sporadic basal-like tumors, we investigated 41 basal-like tumors for BRCA1 mRNA expression by quantitative real-time polymerase chain reaction, BRCA1 promoter methylation, their genomic profile by array-CGH, and gene expression levels by whole genome expression arrays. Array-CGH results were compared to those of 34 proven BRCA1-mutated tumors. Basal-like tumors were subdivided into two equal groups: deficient and proficient in BRCA1 gene expression. The chromosomal makeup of BRCA1 deficient sporadic basal-like tumors was similar to that of BRCA1-mutated tumors. BRCA1 proficient sporadic basal-like tumors were more similar to nonbasal-like tumors. Only half of the basal-like breast tumors are actually deficient in BRCA1 expression. Gain of chromosome arm 3q is a marker for BRCA1 deficiency in hereditary and sporadic breast tumors.
AB - About 10-20% of all breast carcinomas show a basal-like phenotype, while ? 90% of breast tumors from BRCA1-mutation carriers are of this subtype. There is growing evidence that BRCA1-mutated tumors are not just a specific subset of the basal-like tumors, but that (the majority of) basal-like tumors show a dysfunctional BRCA1 pathway. This has major treatment implications, because emerging regimens specifically targeting DNA repair mechanisms would then be most effective against these tumors. To further understand the involvement of BRCA1 deficiency in sporadic basal-like tumors, we investigated 41 basal-like tumors for BRCA1 mRNA expression by quantitative real-time polymerase chain reaction, BRCA1 promoter methylation, their genomic profile by array-CGH, and gene expression levels by whole genome expression arrays. Array-CGH results were compared to those of 34 proven BRCA1-mutated tumors. Basal-like tumors were subdivided into two equal groups: deficient and proficient in BRCA1 gene expression. The chromosomal makeup of BRCA1 deficient sporadic basal-like tumors was similar to that of BRCA1-mutated tumors. BRCA1 proficient sporadic basal-like tumors were more similar to nonbasal-like tumors. Only half of the basal-like breast tumors are actually deficient in BRCA1 expression. Gain of chromosome arm 3q is a marker for BRCA1 deficiency in hereditary and sporadic breast tumors.
KW - Adult
KW - Humans
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Mutation genetics
KW - Chromosome Aberrations
KW - Gene Expression Regulation, Neoplastic
KW - Histones genetics
KW - Breast Neoplasms genetics
KW - Tumor Suppressor Proteins genetics
KW - BRCA1 Protein deficiency
KW - Chromosomes, Human, Pair 3 genetics
KW - DNA Copy Number Variations genetics
KW - DNA Methylation
KW - Neoplasms, Basal Cell genetics
KW - Promoter Regions, Genetic genetics
KW - Adult
KW - Humans
KW - Aged
KW - Female
KW - Middle Aged
KW - Aged, 80 and over
KW - Mutation genetics
KW - Chromosome Aberrations
KW - Gene Expression Regulation, Neoplastic
KW - Histones genetics
KW - Breast Neoplasms genetics
KW - Tumor Suppressor Proteins genetics
KW - BRCA1 Protein deficiency
KW - Chromosomes, Human, Pair 3 genetics
KW - DNA Copy Number Variations genetics
KW - DNA Methylation
KW - Neoplasms, Basal Cell genetics
KW - Promoter Regions, Genetic genetics
M3 - SCORING: Journal article
VL - 50
SP - 71
EP - 81
JO - GENE CHROMOSOME CANC
JF - GENE CHROMOSOME CANC
SN - 1045-2257
IS - 2
M1 - 2
ER -
