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Genetic and functional characterization of isolated stromal cell lines from the aorta-gonado-mesonephros region

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Genetic and functional characterization of isolated stromal cell lines from the aorta-gonado-mesonephros region. / Weisel, Katja C; Moore, Malcolm A S.

in: ANN NY ACAD SCI, Jahrgang 1044, 06.2005, S. 51-9.

Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Review › Forschung

Harvard

Weisel, KC & Moore, MAS 2005, 'Genetic and functional characterization of isolated stromal cell lines from the aorta-gonado-mesonephros region', ANN NY ACAD SCI, Jg. 1044, S. 51-9. https://doi.org/10.1196/annals.1349.007

APA

Weisel, K. C., & Moore, M. A. S. (2005). Genetic and functional characterization of isolated stromal cell lines from the aorta-gonado-mesonephros region. ANN NY ACAD SCI, 1044, 51-9. https://doi.org/10.1196/annals.1349.007

Vancouver

Weisel KC, Moore MAS. Genetic and functional characterization of isolated stromal cell lines from the aorta-gonado-mesonephros region. ANN NY ACAD SCI. 2005 Jun;1044:51-9. https://doi.org/10.1196/annals.1349.007

Bibtex

@article{eefe05a8b2e4414b9a594b04ccf1e48b,
title = "Genetic and functional characterization of isolated stromal cell lines from the aorta-gonado-mesonephros region",
abstract = "The hematopoietic system interacts with a supportive stromal environment allowing maintenance and differentiation of hematopoietic stem cells (HSCs). The aorta-gonado-mesonephros (AGM) region serves as a unique embryonic microenvironment, generating the first adult repopulating HSCs in the mouse embryo. To eludicate factors involved in hematopoietic support and induction of hematopoietic differentiation, we isolated more than 100 stromal cell clones derived from the AGM region of embryonic day (E) 10.5 mouse embryos for functional and genetic analysis. Selected isolated AGM stromal cell lines are highly efficient in supporting maintenance and expansion of mouse and human hematopoietic stem and progenitor cells. In addition, we can demonstrate for the first time that AGM stromal cell lines are also potent inducers of hematopoietic differentiation of murine embryonic stem cells. Stromal gene array analysis has identified genes that could play a role in hematopoietic support.",
keywords = "Animals, Aorta, Cell Line, Coculture Techniques, Embryo, Mammalian, Gonads, Hematopoietic Stem Cells, Humans, Mesonephros, Mice, Stromal Cells, Journal Article, Review",
author = "Weisel, {Katja C} and Moore, {Malcolm A S}",
year = "2005",
month = jun,
doi = "10.1196/annals.1349.007",
language = "English",
volume = "1044",
pages = "51--9",
journal = "ANN NY ACAD SCI",
issn = "0077-8923",
publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - Genetic and functional characterization of isolated stromal cell lines from the aorta-gonado-mesonephros region

AU - Weisel, Katja C

AU - Moore, Malcolm A S

PY - 2005/6

Y1 - 2005/6

N2 - The hematopoietic system interacts with a supportive stromal environment allowing maintenance and differentiation of hematopoietic stem cells (HSCs). The aorta-gonado-mesonephros (AGM) region serves as a unique embryonic microenvironment, generating the first adult repopulating HSCs in the mouse embryo. To eludicate factors involved in hematopoietic support and induction of hematopoietic differentiation, we isolated more than 100 stromal cell clones derived from the AGM region of embryonic day (E) 10.5 mouse embryos for functional and genetic analysis. Selected isolated AGM stromal cell lines are highly efficient in supporting maintenance and expansion of mouse and human hematopoietic stem and progenitor cells. In addition, we can demonstrate for the first time that AGM stromal cell lines are also potent inducers of hematopoietic differentiation of murine embryonic stem cells. Stromal gene array analysis has identified genes that could play a role in hematopoietic support.

AB - The hematopoietic system interacts with a supportive stromal environment allowing maintenance and differentiation of hematopoietic stem cells (HSCs). The aorta-gonado-mesonephros (AGM) region serves as a unique embryonic microenvironment, generating the first adult repopulating HSCs in the mouse embryo. To eludicate factors involved in hematopoietic support and induction of hematopoietic differentiation, we isolated more than 100 stromal cell clones derived from the AGM region of embryonic day (E) 10.5 mouse embryos for functional and genetic analysis. Selected isolated AGM stromal cell lines are highly efficient in supporting maintenance and expansion of mouse and human hematopoietic stem and progenitor cells. In addition, we can demonstrate for the first time that AGM stromal cell lines are also potent inducers of hematopoietic differentiation of murine embryonic stem cells. Stromal gene array analysis has identified genes that could play a role in hematopoietic support.

KW - Animals

KW - Aorta

KW - Cell Line

KW - Coculture Techniques

KW - Embryo, Mammalian

KW - Gonads

KW - Hematopoietic Stem Cells

KW - Humans

KW - Mesonephros

KW - Mice

KW - Stromal Cells

KW - Journal Article

KW - Review

U2 - 10.1196/annals.1349.007

DO - 10.1196/annals.1349.007

M3 - SCORING: Review article

C2 - 15958697

VL - 1044

SP - 51

EP - 59

JO - ANN NY ACAD SCI

JF - ANN NY ACAD SCI

SN - 0077-8923

ER -