Functional localization of an exocytosis-triggering G-protein in human cytotoxic T lymphocytes
Standard
Functional localization of an exocytosis-triggering G-protein in human cytotoxic T lymphocytes. / Mittrücker, H W; Fleischer, B.
in: IMMUNOLOGY, Jahrgang 76, Nr. 4, 01.08.1992, S. 610-5.Publikationen: SCORING: Beitrag in Fachzeitschrift/Zeitung › SCORING: Zeitschriftenaufsatz › Forschung › Begutachtung
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Functional localization of an exocytosis-triggering G-protein in human cytotoxic T lymphocytes
AU - Mittrücker, H W
AU - Fleischer, B
PY - 1992/8/1
Y1 - 1992/8/1
N2 - Human cloned CD8+ cytotoxic T lymphocytes permeabilized with alpha-toxin of Staphylococcus aureus can be triggered by the guanosine triphosphate (GTP) analogue GTP gamma S to release the contents of their granula by exocytosis. To localize the guanosine nucleotide-binding protein (G-protein) activated by GTP gamma S in the sequence of events after T-lymphocyte triggering we have used several inhibitors of T-cell activation that inhibit early stages in T-cell triggering. The protein kinase C-inhibitor staurosporine, the immunosuppressants cyclosporin A and FK-506 and genistein, an inhibitor of tyrosine kinases, all inhibited esterase release triggered in intact cells by anti-T-cell receptor antibodies but not GTP gamma S-induced release from permeabilized cells. Cyclosporin A, FK-506 and genistein also blocked exocytosis triggered in intact cells by a combination of phorbolester and the calcium ionophore A23187. In addition, cytochalasin B, an inhibitor of actin polymerization, inhibited exocytosis in intact cells but enhanced exocytosis from permeabilized cells. These data show that the G-protein effecting exocytosis is localized distally in the cascade of events after T-cell activation.
AB - Human cloned CD8+ cytotoxic T lymphocytes permeabilized with alpha-toxin of Staphylococcus aureus can be triggered by the guanosine triphosphate (GTP) analogue GTP gamma S to release the contents of their granula by exocytosis. To localize the guanosine nucleotide-binding protein (G-protein) activated by GTP gamma S in the sequence of events after T-lymphocyte triggering we have used several inhibitors of T-cell activation that inhibit early stages in T-cell triggering. The protein kinase C-inhibitor staurosporine, the immunosuppressants cyclosporin A and FK-506 and genistein, an inhibitor of tyrosine kinases, all inhibited esterase release triggered in intact cells by anti-T-cell receptor antibodies but not GTP gamma S-induced release from permeabilized cells. Cyclosporin A, FK-506 and genistein also blocked exocytosis triggered in intact cells by a combination of phorbolester and the calcium ionophore A23187. In addition, cytochalasin B, an inhibitor of actin polymerization, inhibited exocytosis in intact cells but enhanced exocytosis from permeabilized cells. These data show that the G-protein effecting exocytosis is localized distally in the cascade of events after T-cell activation.
KW - Alkaloids
KW - Cells, Cultured
KW - Cyclosporine
KW - Exocytosis
KW - GTP-Binding Proteins
KW - Guanosine 5'-O-(3-Thiotriphosphate)
KW - Humans
KW - Lymphocyte Activation
KW - Protein Kinase C
KW - Protein-Tyrosine Kinases
KW - Staurosporine
KW - T-Lymphocytes, Cytotoxic
KW - Tacrolimus
M3 - SCORING: Journal article
C2 - 1383135
VL - 76
SP - 610
EP - 615
JO - IMMUNOLOGY
JF - IMMUNOLOGY
SN - 0019-2805
IS - 4
ER -